Synaptotagmin-4 antibody

Principal name

Synaptotagmin-4 antibody

Alternative names for Synaptotagmin-4 antibody

Synaptotagmin 4, SYT4, SYT-4, Synaptotagmin IV, SytIV, KIAA1342

SwissProt ID

P40749 (Mouse), P50232 (Rat), Q9H2B2 (Human)

Gene ID

6860 (SYT4), 20983 (Syt4), 64440

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of Synaptotagmin-4 antibody

Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles. Calcium binds to Synaptotagmin I which triggers neurotransmitter release at the synapse. Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions. Synaptotagmin III is involved in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons. Synaptotagmin IV is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus. The proximity of the Synaptotagmin IV gene to markers of several psychiatric disorders suggest an involvement of synaptotagmin IV in human disease. Synaptotagmin V is a dense-core vesicle-specific protein that regulates a specific type of calcium-regulated secretion. Synaptotagmin VI interacts with adaptor protein-2 in a calcium-independent manner. Synaptotagmin VII is widely expressed in non-neuronal tissues.

General readings

Ferguson G D., et al. (2000) J Biol Chem. 275:36920-36926.Ibata K., et al. (2002) Neurosci Res. 43: 401-406.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Synaptotagmin-4 antibody

  SYT4 mouse monoclonal antibody,clone UMAB128  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-SYT4 mouse monoclonal antibody. (UM500093; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB128 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Synaptotagmin-4 antibody

  SYT4 mouse monoclonal antibody,clone UMAB141  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-SYT4 mouse monoclonal antibody. (UM500103, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB141 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Synaptotagmin-4 antibody

  SYT4 mouse monoclonal antibody,clone UMAB128  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-SYT4 mouse monoclonal antibody. (UM500093; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB128 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Synaptotagmin-4 antibody

  SYT4 mouse monoclonal antibody,clone UMAB141  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-SYT4 mouse monoclonal antibody. (UM500103, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB141 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI2F2 (formerly 2F2)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SYT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505848) Mouse IgG2b OTI2F2 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SYT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505862) Mouse IgG2b OTI4H3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  Rabbit Polyclonal Anti-SYT4 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, Jurkat cells, Primary antibody: (SYT4 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI3E10 (formerly 3E10)  
Anti-SYT4 mouse monoclonal antibody (TA505846) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SYT4(RC206304). Mouse IgG1 OTI3E10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI2B8 (formerly 2B8)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SYT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505847) Mouse IgG2b OTI2B8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SYT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505849) Mouse IgG2b OTI2D3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI3D4 (formerly 3D4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SYT4 (RC206304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYT4. Mouse IgG2b OTI3D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  SYT4 mouse monoclonal antibody, clone OTI4F2 (formerly 4F2)  
Anti-SYT4 mouse monoclonal antibody (TA505863) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SYT4(RC206304). Mouse IgG1 OTI4F2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Synaptotagmin-4 antibody

  SYT4 (Synaptotagmin 4) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SYT4 (RC206304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYT4. Mouse IgG2b OTI3B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

SYT4 - human gene knockout kit via CRISPR

SYT4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SYT4 - human gene knockout kit via CRISPR

SYT4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SYT4 - human gene knockout kit via CRISPR

SYT4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SYT4 - human gene knockout kit via CRISPR

SYT4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Syt4 - mouse gene knockout kit via CRISPR

Syt4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Syt4 - mouse gene knockout kit via CRISPR

Syt4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Synaptotagmin-4

  Recombinant protein of human synaptotagmin IV (SYT4)  
Synaptotagmin-4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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