Synaptotagmin-6 antibody

Principal name

Synaptotagmin-6 antibody

Alternative names for Synaptotagmin-6 antibody

Synaptotagmin 6, SYT6, SYT-6, Synaptotagmin VI, SytVI

SwissProt ID

Q5T7P8 (Human), Q62746 (Rat), Q9R0N8 (Mouse)

Gene ID

148281 (SYT6)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Synaptotagmin-6 antibody

Synaptotagmins constitute a family of membranetrafficking proteins that are characterized by an N- terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins.  The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).
Synaptotagmin-6 is localized to the endoplasmic reticulum and/or Golgi-like perinuclear compartment. It may be important for trafficking and calcium signaling as it is specially expressed in non-neuronal tissues. Also SytVI is present in the acrosomal region of mammalian spermatozoa. The cytosolic domain of SytVI can abrogate exocytosis by competing with the endogenous protein for essential interactions with the fusion machinery involved in a acrosomal exocytosis (3-5).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Synaptotagmin-6 antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SYT6 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Synaptotagmin-6 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using SYT6 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Synaptotagmin-6 (246-333, Cytopl. Dom.) antibody

WB of rat brain lysate showing detection of ~55 kDa Synaptotagmin 6 protein using Anti-Synaptotagmin 6.  Lane 1: MW ladder. Lane 2: Anti-Synaptotagmin 6 (1:100). Lane 3: Anti-Synaptotagmin 6 (1:250). Lane 4: Anti-Synaptotagmin 6 (1:500). Lane 5: Anti-Synaptotagmin 6 (1:1000).  Load: 20 µg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-Synaptotagmin 6 monoclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Mouse IgG1 S270-47 Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Synaptotagmin-6 antibody

Adult rat brain membrane immunoblot Mouse IgG1 N270/47 Supernatant Hu, Ms, Rt ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

Synaptotagmin-6 antibody

Adult rat brain membrane immunoblot Mouse IgG1 N270/47 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

Synaptotagmin-6 antibody

Immunoblot analysis cell and tissue lysates. Lane 1 : SH-SY5Y cell lysate, lane 2 : mouse liver tissue lysate and lane 3 : rat liver tissue lysate. ( * N. S : Nonspecific band ) Rabbit IgG Purified Hu C, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Synaptotagmin-6

Synaptotagmin-6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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