t-PA / PLAT antibody

Principal name

t-PA / PLAT antibody

Alternative names for t-PA / PLAT antibody

tPA, Tissue-type plasminogen activator, Alteplase, Reteplase

SwissProt ID

P00750 (Human), P00750-3, P11214 (Mouse), P19637 (Rat), Q28198 (Bovin), Q8MKB1 (Rabit), Q8SQ23 (Pig)

Gene ID

5327 (PLAT), 18791 (Plat), 100009556

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Rb (Rabbit), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat, Sheep

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), ELISA (detection) (E(detection)), Radioimmunoassay (R)

Background of t-PA / PLAT antibody

TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. It specifically cleaves the Arg-|-Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

t-PA / PLAT antibody

t-PA / PLAT Goat Serum Hu C, E, P
0.1 ml / €220.00
  Acris Antibodies GmbH

t-PA / PLAT antibody

t-PA / PLAT Goat Serum Hu C, E, P
20 µl / €120.00
  Acris Antibodies GmbH

t-PA / PLAT antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805448) Mouse IgG2b OTI3H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

t-PA / PLAT antibody

Predicted band size: 63 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

t-PA / PLAT antibody

Predicted band size: 63 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

t-PA / PLAT antibody

Western blot analysis of extracts of various cell lines, using PLAT antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

t-PA / PLAT antibody

WB Suggested Anti-PLAT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

t-PA / PLAT antibody

t-PA / PLAT Mouse IgG 2A153 Purified Hu E, WB
0.2 mg / €920.00
  Acris Antibodies GmbH

t-PA / PLAT antibody

t-PA / PLAT Rabbit Purified Hu C, E, WB
0.2 mg / €460.00
  Acris Antibodies GmbH

t-PA / PLAT (Center) antibody

Western blot analysis of TPA Antibody (Center) (AP17801PU-N) in A2058 cell line lysates (35ug/lane). TPA (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

t-PA / PLAT antibody

Western Blot analysis of PLAT expression in transfected 293T cell line (H00005327-T01) by PLAT MaxPab polyclonal antibody.

Lane 1: PLAT transfected lysate(61.82 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

t-PA / PLAT Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

Western Blot analysis of PLAT expression in transfected 293T cell line (H00005327-T01) by PLAT MaxPab polyclonal antibody.

Lane 1: PLAT transfected lysate(62.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

t-PA / PLAT Mouse IgG H27B6 Ms, Rt C, E, WB
1 mg / €1,080.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

Lane 1. Recombinant mouse Plat.
Lane 2. Recombinant mouse Plat in complex with Serpine1. Mouse IgG H6C5 Ms E, WB
1 mg / €1,080.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

Lane 1. Human PLAT.
Lane 2. PLAT in complex with Serpine1.
Lane 3. Retavase Boehringer/Mannheim ( PLAT fragment ).
Lane 4. Retavase in complex with Serpine1. Mouse IgG 629-2A153 Hu E, WB
1 mg / €730.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

t-PA / PLAT Rabbit Ms E, R, WB
0.4 mg / €390.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

t-PA / PLAT Rabbit Ms E, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

t-PA / PLAT antibody

t-PA / PLAT Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

t-PA / PLAT antibody

Western Blot: Tissue Plasminogen Activator Antibody [NB110-68110] - Rabbit anti Mouse tPA (2ug/ml)  Secondary: anti Rabbit IgG ABC Kit  Lane 1: 0.5ug mouse tPA (non-reduced)  Lane 2: 0.5ug mouse tPA + 3ug CPAI (non-reduced)  Lane 3: 0.5ug mouse tPA (reduced)  Lane 4: 0.5ug mouse tPA + 3ug CPAI (reduced)  Lane 5: Molecular weight standards Rabbit Purified Ms, Rt E, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

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