TAF1 antibody

Principal name

TAF1 antibody

Alternative names for TAF1 antibody

BA2R, CCG1, CCGS, TAFII250, Transcription initiation factor TFIID subunit 1, Transcription initiation factor TFIID 250 kDa subunit, TBP-associated factor 250 kDa, Cell cycle gene 1 protein

SwissProt ID

P21675 (Human), Q80UV9 (Mouse)

Gene ID

6872 (TAF1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TAF1 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF1 encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.

General readings

Herzfeld,T., (2007) Mamm. Genome 18 (11), 787-795

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TAF1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TAF1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TAF1 antibody

Western blot using affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TAF1 antibody

WB Suggested Anti-TAF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TAF1 antibody

WB Suggested Anti-TAF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Daudi cell lysateTAF1 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TAF1 antibody

WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human Med26 TAF1, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

TAF1 antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bear, Bov, Can, Chk, Eq, Hst, Hu, Mky, Ms, P. aeruginosa, Prim, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

TAF1 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, P
50 µg / €470.00
  Acris Antibodies GmbH

TAF1 antibody

MCF7 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with Rabbit anti-TAF1 Antibody (TAF250). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In addition to the TAF250 specific band, this polyclonal antibody detects an unknown protein with a molecular weight of approximately 50 kD. Rabbit IgG Aff - Purified Hu WB
0.2 ml / €360.00
  Acris Antibodies GmbH

TAF1 (C-term) antibody

TAF1 Antibody (C-term) (Cat. #AP54141PU-N) western blot analysis in mouse NIH-3T3 cell line lysates (35µg/lane).This demonstrates the TAF1 antibody detected the TAF1 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TAF1 antibody

Detection limit for recombinant GST tagged TAF1 is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 1E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TAF1 antibody

TAF1 monoclonal antibody (M02), clone 1G9 Western Blot analysis of TAF1 expression in Hela NE ( Cat # L013V3 ). Mouse IgG 1G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TAF1 antibody

Western blot using TAF1 polyclonal antibody ( Cat # PAB11285 ) to detect TAF1 in HeLa nuclear extract ( arrowhead ).
The membrane was probed with the primary antibody at dilutions of 1 : 100 ( lane 1 ), 1 : 250 ( lane 2 ), 1 : 500 ( Lane 3 ) and 1 : 1,000 ( Lane 4 ).
The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1.
Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD. Rabbit Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

TAF1 antibody

Western blot was performed using nuclear extracts from HeLa cells ( HeLa NE, 20 µg ) and TAF1 polyclonal antibody ( Cat # PAB14052 ) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. Rabbit Hu WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

TAF1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TAF1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

TAF1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TAF1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

TAF1 antibody

Human Liver; WB Suggested Anti-TAF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; TAF1 antibody - middle region (ARP32472_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TAF1 antibody

Human kidney; TAF1 antibody - C-terminal region (ARP32473_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

TAF1 antibody

TAF1 Mouse 2A16 Purified Hu ELISA
0.2 mg / €990.00
  Funakoshi Co., Ltd.

TAF1 antibody

TAF1 Mouse 10G1 Purified Hu ELISA
0.2 mg / €990.00
  Funakoshi Co., Ltd.

TAF1 antibody

TAF1 Mouse 10G1 HRP Hu ELISA
2 ml / €990.00
  Funakoshi Co., Ltd.

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