TBP (TATA-box-binding protein) antibody

Principal name

TBP (TATA-box-binding protein) antibody

Alternative names for TBP (TATA-box-binding protein) antibody

TATA-box factor, TATA-binding factor, GTF2D1, TF2D, TFIID, Transcription initiation factor TFIID TBP subunit

SwissProt ID

P20226 (Human)

Gene ID

6908 (TBP)

Available reactivities

Ce (C. elegans), Hu (Human), Ms (Mouse), Rt (Rat), Mam (Mammalia), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Por (Porcine), Rb (Rabbit), Chimp (Chimpanzee), Xen (Xenopus), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ChIP assay (ChIP), Electrophoretic Mobility Shift Assay (EMSA), Flow Cytometry (F), Paraffin Sections (P)

Background of TBP (TATA-box-binding protein) antibody

Initiation of transcription by R polymerase II requires the activities of more than 70 polypeptides. The protein that coordites these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory sigls. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutiorily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-termil. This region of the protein modulates the D binding activity of the C terminus, and modulation of D binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. [provided by RefSeq]

General readings

Reid,S.J., et al., (2004) Brain Res. Mol. Brain Res. 125 (1-2), 120-128

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TBP (TATA-box-binding protein) antibody

TBP (TATA-box-binding protein) Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) (Isoform 1) antibody

(0.3ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in commercial extraction buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) antibody

Western blot analysis of extracts from 293 cells, using TBP antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) antibody

TBP Antibody (Cat. #TA324501) western blot analysis in Hela,HepG2,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow). Mouse IgG1 830CT4.3.3 Purified Hu, Ms ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) antibody

TBP Antibody (Center) (Cat. #TA324859) western blot analysis in K562,U251 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow). Rabbit IgG Purified Bov, Chk, Hu, Mky, Ms, Xen, Ze ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) antibody

WB Suggested Anti-TBP Antibody Titration: 2.5ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) antibody

ChIP assays using U2OS cells, the TBP ab and primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 ug ab were used. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Image shows the recovery (the relative amount of IP'd DNA compared to input DNA) and the occupancy (ratio +/- control target): the occupancy of both promoters by TBP. Mouse IgG Monoclol Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) (39-50) antibody

TA348995 (1 ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBP (TATA-box-binding protein) (C-term) antibody

Western blot analysis of TBP antibody (C-term) (AP17778PU-N) in HL60 cell line lysates (35ug/lane). TBP (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TBP (TATA-box-binding protein) antibody

TBP (TATA-box-binding protein) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TBP (TATA-box-binding protein) antibody

TBP (TATA-box-binding protein) Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

TBP (TATA-box-binding protein) antibody

ChIP results obtained with the TBP monoclonal antibody ( Cat # MAB0121 ) . ChIP assays were performed using U-2 OS cells. Mouse IgG1 Hu ChIP
0.1 mg / €470.00
  Abnova Taiwan Corp.

TBP (TATA-box-binding protein) antibody

Western blot analysis of extracts from 293 cells, using TBP Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

TBP (TATA-box-binding protein) antibody

Western blot analysis of extracts from 293 cells, using TBP Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

TBP (TATA-box-binding protein) antibody

Human Placenta; WB Suggested Anti-TBP Antibody Titration: 2.5ug/ml. Positive Control: Human Placenta; TBP antibody - middle region (P100971_T100) in Human Placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

TBP (TATA-box-binding protein) antibody

TBP (TATA-box-binding protein) Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

TBP (TATA-box-binding protein) antibody

Immunohistochemical analysis of TBP staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rb, Rt, Ze ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TBP (TATA-box-binding protein) antibody

Immunohistochemical analysis of TBP staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rb, Rt, Ze ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TBP (TATA-box-binding protein) antibody

Immunohistochemical analysis of TBP staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rb, Rt, Ze ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

TBP (TATA-box-binding protein) antibody

Western blot analysis of TBP expression in Hela (A); mouse brain (B); rat brain (C) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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