TBX21 / TBET antibody

Principal name

TBX21 / TBET antibody

Alternative names for TBX21 / TBET antibody

T-bet, TBLYM

SwissProt ID

Q9JKD8 (Mouse), Q9UL17 (Human)

Gene ID

30009 (TBX21), 57765 (Tbx21)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TBX21 / TBET antibody

T-bet, a T-box transcription factor, is expressed in CD4+ T-lymphocytes committed to T-helper (Th)1 T-cell development from naïve T-helper precursor cells (Thp) and redirects Th2 T cells to Th1 development. Anti-T-bet is a marker of mature T-cells and is expressed at very low levels in Thp cells and is absent in precursor T-lymphoblastic leukemia/lymphoma cells. Scattered small lymphocytes in the interfollicular T-cell zone of reactive lymphoid tissue, including tonsil, lymph node, and spleen exhibited nuclear staining for T-bet, with no T-bet staining observed in germinal centers or mantle or marginal zones.
T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B cells, including CLL/SLL, marginal zone lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pregerminal center or germinal center B-cells, including mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma are negative for T-bet. Therefore, anti-T-bet should serve as a useful marker for the diagnosis and subtyping of B-cell and T-cell lymphoproliferative disorders.

General readings

Johrens,K., et al., (2006) Histopathology 48 (4), 343-352

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TBX21 / TBET antibody

  Mouse Anti-T-bet Purified (25 ug)  
CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP. Mouse eBio39D (39D, 3-9D) Purified Hu, Ms, Rt IP, WB
25 µg / €120.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Mouse Anti-Human/Mouse T-bet Purified (25 ug)  
CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right) cells were probed with Anti-Human/Mouse T-bet Purified at 2 ug/mL, and revealed with Anti-Mouse IgG HRP. Mouse eBio4B10 (4B10, 4-B10) Purified Hu, Ms IP, P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit polyclonal TBX21 Antibody (N-term)  
TBX21 Antibody (N-term) (Cat. #TA324549) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TBX21 antibody detected the TBX21 protein (arrow). Rabbit IgG Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit Polyclonal TBX21 Antibody  
Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at  1 ug/ml. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit Monoclonal T-bet Antibody  
Immunohistochemistry staining of Paraffin Tonsil tissue by T-bet antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear) Rabbit IgG1 MRQ-46 Purified Hu P
1 ml / €860.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit Polyclonal Anti-TBX21 Antibody  
WB Suggested Anti-TBX21 Antibody Titration: 2.5ug/ml; ELISA Titer: 1:62500; Positive Control: Human Thymus Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit Polyclonal Anti-TBX21 Antibody  
WB Suggested Anti-TBX21 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit Polyclonal Anti-Tbx21 Antibody  
WB Suggested Anti-Tbx21 Antibody Titration: 0.2-1 ug/ml; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

  Rabbit Polyclonal Anti-TBX21 Antibody  
WB Suggested Anti-TBX21 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TBX21 / TBET antibody

TBX21 / TBET Rabbit IgG1 MRQ-46 Supernatant Hu C, P
1 ml / €1,030.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TBX21 - human gene knockout kit via CRISPR

TBX21 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TBX21 - human gene knockout kit via CRISPR

TBX21 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TBX21 - human gene knockout kit via CRISPR

TBX21 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TBX21 - human gene knockout kit via CRISPR

TBX21 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tbx21 - mouse gene knockout kit via CRISPR

Tbx21 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Tbx21 - mouse gene knockout kit via CRISPR

Tbx21 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tbx21 - mouse gene knockout kit via CRISPR

Tbx21 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tbx21 - mouse gene knockout kit via CRISPR

Tbx21 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TBX21 / TBET

  Recombinant protein of human T-box 21 (TBX21)  
TBX21 / TBET Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

TBX21 overexpression lysate

TBX21 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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