TCF4 antibody

Principal name

TCF4 antibody

Alternative names for TCF4 antibody

Transcription factor 4, TCF-4, ITF2, ITF-2, SEF2, SEF-2, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2

SwissProt ID

P15881 (Canfa), P15884 (Human), Q60722 (Mouse), Q62655 (Rat), Q90683 (Chick)

Gene ID

6925 (TCF4), 6934 (TCF7L2)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Electrophoretic Mobility Shift Assay (EMSA), Paraffin Sections (P)

Background of TCF4 antibody

T cell transcription factor-4 (TCF-4) is a member of the TCF-LEF family of transcription factors. TCF-4 interacts with b-Catenin in the Wnt signaling pathway to activate transcription of Wnt target genes. TCF-4 plays important roles in embryogenesis and oncogenesis. High levels of TCF-4 expression was found in developing CNS and limb buds. (In adults, TCF-4 is widely expressed.) Brain is the major site of expression, however, TCF-4 mRNA is also found in high levels in liver, lower in heart, lungs, kidneys, and testis, and lowest in spleen and muscle. In human TCF-4, four alternative splice sites are observed which correspond to the synthesis of many hTCF-4 isoforms with short, medium, or long-C-terminal ends (ADDINENRfu). BLAST searches showed these isoforms are very similar to each other, except those that are close to the C-terminus, however, the calculated molecular weights ranged from 52kD to 68kD. For mouse TCF-4, BLAST searches showed different isoforms with calculated molecular weights that range from 29kD to 51kD.

General readings

General / background references:Barker N et al. Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium. Am J Pathol 154:29-35 (1999). PubMed PMID: 9916915

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TCF3 / TCF4 antibody

Figure 1. Western blot analysis using AM05272PU-N antibody at 4 µg/ml on Cos-1 cell lysate control (Lane 1) and Cos-1 cells transfected with human TCF-3 (Lane 2). Mouse IgG1 6F12.3 Purified Hu, Ms EMSA, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

TCF3 / TCF4 antibody

TCF3 / TCF4 Mouse IgG 6F12-3 Ascites Hu, Ms C, P, WB
25 µg / €360.00
  Novus Biologicals Inc.

TCF4 antibody

Figure 1. Western blot analysis using AM05271PU-N antibody at 1 µg/ml on Cos-1 cell lysate control (Lane 1) and Cos-1 cells transfected with human TCF-4 (Lane 2). Mouse IgG2a 6H5.3 Purified Hu, Ms IP, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

TCF4 antibody

AP32964PU-N (0.5µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TCF4 antibody

Western blot analysis of extracts from HUVEC cells, using TCF4/12 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TCF4 antibody

Predicted band size: 71 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TCF4 antibody

Western blot analysis of K562 cell lysate using TCF4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TCF4 antibody

WB Suggested Anti-TCF4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TCF4 antibody

WB Suggested Anti-TCF4 Antibody; Titration: 0.2-1 ug/ml; Positive Control: HepG2 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TCF4 antibody

Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferus duct cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu ICC/IF, P
50 µg / €325.00
  OriGene Technologies, Inc.

TCF4 antibody

TCF4 Rabbit IgG Purified Hu ICC/IF, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

TCF4 antibody

Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3E10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TCF4 antibody

TCF4 monoclonal antibody (M02), clone 1F6 Western Blot analysis of TCF4 expression in A-549 ( Cat # L025V1 ). Mouse IgG1 1F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TCF4 antibody

Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1G4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TCF4 antibody

Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3H4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TCF4 antibody

Western blot analysis of TCF4 expression in COS-1 tissue control ( lane 1 ) and COS-1 cells transfected with human TCF4 ( lane 2 ) probed with TCF4 monoclonal antibody, clone 6H5-3 at 1 µg/ml. Mouse IgG2a 6H5-3 Hu, Ms C, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

TCF4 antibody

Western blot analysis of extracts from HuvEc cells, using TCF4/12 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

TCF4 antibody

Western blot analysis of extracts from HuvEc cells, using TCF4/12 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

TCF4 antibody

Human HT1080; Host: Rabbit. Target Name: TCF4. Sample Tissue: HT1080 Whole Cell lysates. Antibody Dilution: 1.0ug/ml. Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

TCF4 antibody

human neuroblastoma; TCF4 antibody - N-terminal region (P100776_P050) validated by WB using 1. SHSY-5Y lysate (15ug) at 1:100.. sH-SY5Y cell lysate;no + transfected hTCF4;yes (human neuroblastoma cells); TCF4 antibody - N-terminal region (P100776_P050) in human neuroblastoma cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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