TDG antibody

Principal name

TDG antibody

Alternative names for TDG antibody

G/T mismatch-specific thymine DNA glycosylase

SwissProt ID

P56581 (Mouse), Q13569 (Human)

Gene ID

6996 (TDG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), ChIP assay (ChIP), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of TDG antibody

In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.

General readings

Neddermann, P ., et al. (1996) J Biol Chem 271(22): 12767-74
Sard, L., et al. (1997) Genomics 44(2): 222-6
Gallinari, P., et al. (1996) Nature 383(6602): 735-8

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TDG antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150) Mouse IgG1 OTI3A12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TDG antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150) Mouse IgG1 OTI3C11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TDG antibody

Western blot analysis of extracts from Jurkat cells, using TDG antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TDG antibody

Western blot analysis of extracts of various cell lines, using TDG antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

TDG antibody

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2F7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

TDG antibody

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2F7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

TDG antibody

Western blot (WB) analysis of TDG antibody (Cat.-No.: AP06727PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:Mouse lung tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TDG (C-term) antibody

Western blot analysis of TDG antibody (C-term) (AP17780PU-N) in K562 cell line lysates (35ug/lane). TDG (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TDG (N-term) antibody

Western blot analysis of TDG antibody (N-term) (AP17781PU-N) in mouse lung tissue lysates (35ug/lane). TDG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TDG antibody

Immunofluorescence of purified MaxPab antibody to TDG on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

TDG antibody

Western Blot analysis of TDG expression in transfected 293T cell line (H00006996-T01) by TDG MaxPab polyclonal antibody.

Lane 1: TDG transfected lysate(46.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TDG antibody

Western blot analysis of extracts from Jurkat cells, using TDG Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

TDG antibody

Western blot analysis of extracts from Jurkat cells, using TDG Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

TDG antibody

Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TDG antibody

Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TDG antibody

Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

TDG antibody

Western Blot: TDG Antibody [NB100-92376] - Western blot (WB) analysis of TDG (K90) antibody in  extracts from Jurkat cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

TDG antibody

TDG Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

TDG antibody

TDG Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TDG (1-410, His-tag)

TDG Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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