TDG antibody

Principal name

TDG antibody

Alternative names for TDG antibody

G/T mismatch-specific thymine DNA glycosylase

SwissProt ID

P56581 (Mouse), Q13569 (Human)

Gene ID

6996 (TDG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of TDG antibody

In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.

General readings

Neddermann, P ., et al. (1996) J Biol Chem 271(22): 12767-74
Sard, L., et al. (1997) Genomics 44(2): 222-6
Gallinari, P., et al. (1996) Nature 383(6602): 735-8

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TDG antibody

  TDG mouse monoclonal antibody, clone OTI3A12 (formerly 3A12)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150) Mouse IgG1 OTI3A12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TDG antibody

  TDG mouse monoclonal antibody, clone OTI3C11 (formerly 3C11)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150) Mouse IgG1 OTI3C11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TDG antibody

  Rabbit polyclonal anti-TDG antibody  
Western blot analysis of extracts from Jurkat cells, using TDG antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TDG antibody

  Rabbit anti-TDG Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using TDG antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

TDG antibody

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2F7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

TDG antibody

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2F7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

TDG antibody

Western blot (WB) analysis of TDG antibody (Cat.-No.: AP06727PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:Mouse lung tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TDG (C-term) antibody

Western blot analysis of TDG antibody (C-term) (AP17780PU-N) in K562 cell line lysates (35ug/lane). TDG (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TDG (N-term) antibody

Western blot analysis of TDG antibody (N-term) (AP17781PU-N) in mouse lung tissue lysates (35ug/lane). TDG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TDG antibody

Western blot analysis of extracts from Jurkat cells, using TDG Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

TDG antibody

Western blot analysis of extracts from Jurkat cells, using TDG Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

TDG - human gene knockout kit via CRISPR

TDG - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TDG - human gene knockout kit via CRISPR

TDG - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TDG - human gene knockout kit via CRISPR

TDG - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TDG - human gene knockout kit via CRISPR

TDG - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TDG (1-410, His-tag)

TDG Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

TDG (1-410, His-tag)

TDG Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TDG (mGFP-tagged) - Human thymine-DNA glycosylase (TDG), 200 uL, >10^7 TU/mL

Lenti ORF particles, TDG (mGFP-tagged) - Human thymine-DNA glycosylase (TDG), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, TDG (Myc-DDK tagged) - Human thymine-DNA glycosylase (TDG) , 200 uL, >10^7 TU/mL

Lenti ORF particles, TDG (Myc-DDK tagged) - Human thymine-DNA glycosylase (TDG) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

19 Item(s)

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