TDGF1 / CRIPTO antibody

Principal name

TDGF1 / CRIPTO antibody

Alternative names for TDGF1 / CRIPTO antibody

Cripto-1 growth factor, CRGF, Teratocarcinoma-derived growth factor 1, Epidermal growth factor-like cripto protein CR1

SwissProt ID

P13385 (Human), P51865 (Mouse)

Gene ID

6997 (TDGF1), 100047300 (LOC100047300), 21667 (Tdgf1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Ze (Zebrafish), Can (Canine), Rt (Rat), Prim (Primate)

Available hosts

Rabbit, Goat, Mouse, Rat

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Neutralisation (NEUT)

Background of TDGF1 / CRIPTO antibody

Cripto-1 is the founding member of the EGF-CFC gene family, which is conserved among vertebrates. Proteins within this family contain a signal sequence, a characteristic EGF-like domain, a cysteine-rich region termed the cryptic (CFC) motif, and a hydrophobic Cterminus. It has been reported that Cripto-1 is O-fucosylated on Thr-88 and that this modification is required for the physical interaction with Nodal as well as for signalling activity. We have confirmed that Cripto-1B is O-fucosylated at this site. Cripto-1 has been shown to play a critical role in the formation of the primary germ layers during gastrulation, as well as the specification of the anterior-posterior and left-right body axes in early embryonic development. During embryogenesis, cripto-1 functions as an essential co-receptor for nodal, a member of the transforming growth factor beta (TGF-beta) family, to mediate transcriptional responses. The overexpression of cripto-1 has been identified in many human cancers, including breast, colon, lung, testicular, stomach, pancreatic, and ovarian cancers. The molecular mechanisms by which cripto-1 promotes tumourigenesis is unknown. However, cripto-1 has been shown to participate in in vitro transformation,migration, invasion, epithelial-to-mesenchymal transition, and branching morphogenesis in mammary epithelial cells and in breast and cervical carcinoma cells. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

1. Parisi S, D\1Andrea D, Lago CT, Adamson ED, Persico MG, Minchiotti G Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells. J Cell Biol. 2003 Oct 27;163(2):303-14
PMID: 14581455

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TDGF1 / CRIPTO antibody

IHC Image for Cat#AP15504PU-S / AP15504PU-N Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO antibody

IHC Image for Cat#AP15504PU-S / AP15504PU-N Rabbit Purified Hu P
1 ml / €440.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO antibody

IHC Image for Cat#AP15504PU-S / AP15504PU-N Rabbit Purified Hu P
0.1 ml / €180.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO antibody

Western Blot: Cripto1 Antibody - Analysis of Cripto in MDA-MB231 lysates. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TDGF1 / CRIPTO antibody

WB using Anti-Cripto-1 shows detection of endogenous and transfected Cripto-1 from mouse or human. Endogenous detection using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. Dilution: 1:500 . Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TDGF1 / CRIPTO antibody

Western blot analysis of TDGF1 in 293 cell lysate with TDGF1 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TDGF1 / CRIPTO antibody

Western Blot: Cripto1 Antibody [TA336263] - Analysis of Cripto in Dicer knockout mouse embryonic stem cell lysate. Rabbit Purified Hu, Ms F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TDGF1 / CRIPTO antibody

ELISA: Cripto1 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

TDGF1 / CRIPTO antibody

Western blot analysis using TDGF1 mAb against HEK293 (1) and TDGF1(AA: 31-188)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 3A6 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO antibody

WB using Anti-Cripto-1 shows detection of endogenous and transfected Cripto-1 from mouse or human. Endogenous detection using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. Dilution: 1:500 . Rabbit IgG Aff - Purified Hu, Ms E, WB
25 µl / €190.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO (N-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO antibody

Immunohistochemistry: AP20147PU-N CRIPTO Antibody staining of Paraffin Embedded Human Placenta at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P
0.1 mg / €340.00
  Acris Antibodies GmbH

TDGF1 / CRIPTO antibody

TDGF1 / CRIPTO Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TDGF1 / CRIPTO antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with TDGF1 polyclonal antibody ( Cat # PAB3871 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

TDGF1 / CRIPTO antibody

Western blot using TDGF1 polyclonal antibody ( Cat # PAB9995 ) shows detection of endogenous and transfected TDGF1 from mouse and human sources.
The TDGF1 band appears above the 17.8 kDa marker.
Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells.
EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse TDGF1.
NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose TDGF1 expression.
COS-7 cells transfected with human TDGF1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control.
A non-specific band at 45 kDa may be present in some preparations.
The primary antibody was used at a 1 : 500 dilution.
Personal communication, C. Bianco, NCI, Bethesda, MD. Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

TDGF1 / CRIPTO antibody

Western blot analysis of Tdgf1 in 20 µg of MDA-MB-21 lysate with Tdgf1 polyclonal antibody ( Cat# PAB12003 ). Rabbit Hu WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

TDGF1 / CRIPTO antibody

Western blot analysis of TDGF1 in 20 µg of MDA-MB-21 lysate with TDGF1 polyclonal antibody ( Cat# PAB12004 ). Rabbit Hu WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

TDGF1 / CRIPTO (Center) antibody

Human Jurkat; WB Suggested Anti-TDGF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; TDGF1 antibody - middle region (ARP45440_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TDGF1 / CRIPTO antibody

Immunocytochemistry/Immunofluorescence: Cripto1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue) and tubulin was stained with alpha tubulin (red). Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Prim, Rt F, ICC/IF, WB
0.1 ml / €380.00
  Neuromics Antibodies

TDGF1 / CRIPTO antibody

ELISA: Cripto1 Antibody [32580002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

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