Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Tektin-1 (TEKT1) antibody
This gene product belongs to the tektin family of proteins. Tektins comprise a family of filament-forming proteins that are coassembled with tubulins to form ciliary and flagellar microtubules. This gene is predominantly expressed in the testis and in mouse, tektin 1 mRNA was localized to the spermatocytes and round spermatids in the seminiferous tubules, indicating that it may play a role in spermatogenesis. [provided by RefSeq]
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TEKT1 polyclonal antibody ( Cat # PAB3395 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of TEK ( arrow ) using rabbit TEKT1 polyclonal antibody ( Cat # PAB3395 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the TEK gene ( Lane 2 ) ( Origene Technologies ) .
Dot blot analysis of TEKT1 (phospho Y1113) polyclonal antibody ( Cat # PAB8171 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.