TER ATPase / VCP antibody

Principal name

TER ATPase / VCP antibody

Alternative names for TER ATPase / VCP antibody

15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, Transitional endoplasmic reticulum ATPase

SwissProt ID

P03974 (Pig), P23787 (Xenla), P46462 (Rat), P55072 (Human), Q01853 (Mouse), Q3ZBT1 (Bovin), Q7KN62 (Drome), Q7ZU99 (Danre)

Gene ID

7415 (VCP), 5327197 (IPR007158), 327197 (vcp)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Xen (Xenopus), Por (Porcine), Ze (Zebrafish), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E)

Background of TER ATPase / VCP antibody

P97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. 
Structure: Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
Interaction: VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP.
Bioactivity/Activities: VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.

General readings

1. Peknicova J, Kyselova V, Buckiova D, Boubelik M.: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
2. Pavlasek J, Peknicova J, Ulcova-Gallova Z, Novakova P, Reischig J, Micanova Z, Rokyta Z.: Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction. Ceska Gynekol. 2004 Jul;69(4):306-11.
3. Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
4. Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J.: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
5. Peknicova J, Chladek D, Hozak P.: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis.
Am J Reprod Immunol. 2005 Jan;53(1):42-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TER ATPase / VCP antibody

Formalin-Fixed Paraffin-Embedded Human Prostate tissue stained with p97/VCP Antibody Cat.-No AM08335PU (Clone 4G9)  at 5 µg/ml after heat-induced antigen retrieval. Mouse IgG1 4G9 Aff - Purified Hu ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Formalin-Fixed Paraffin-Embedded Human Prostate tissue stained with p97/VCP Antibody Cat.-No AM08335PU (Clone 4G9)  at 5 µg/ml after heat-induced antigen retrieval. Mouse IgG1 4G9 Aff - Purified Hu ICC/IF, IP, P, WB
25 µg / €220.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

VCP antibody detects VCP protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VCP antibody (TA308570) dilution: 1:1000 Rabbit IgG Purified Bov, Chk, Hu, Ms, Por, Xen, Ze ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Immunohistochemical analysis of paraffin-embedded breast ca, using VCP(GTX101089) antibody at 1:500 dilution. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

 
Sample size available for 25µl / 119€
Need more? Get any 5 sample sizes for only €420!
read more…

TER ATPase / VCP antibody

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

 
Sample size available for 25µl / 119€
Need more? Get any 5 sample sizes for only €420!
read more…

TER ATPase / VCP antibody

VCP antibody [N3C2], Internal detects VCP protein at nucleus on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. VCP antibody [N3C2], Internal (GTX113099) dilution: 1:500. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

 
Sample size available for 25µl / 119€
Need more? Get any 5 sample sizes for only €420!
read more…

TER ATPase / VCP antibody

Western blot analysis of extracts from HeLa cells, using VCP (Ab-784) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Predicted band size: 90 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9 Mouse IgG1 4G9 Purified Hu ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

TA329667 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

TA329668 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

WB Suggested Anti-VCP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Figure 1. Immunofluorescence analysis of 220 kDa intra-acrosomal protein in Acetone-permeabilized Human sperms using monoclonal antibody AM03029 Clone Hs-14 (normal spermiogram). Mouse IgM Hs-14 FITC Hu, Ms F
50 Tests / €340.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Immunofluorescence analysis of 220 kDa intra-acrosomal protein in acetone-permeabilized human sperms using monoclonal antibody Hs-14 (normal spermiogram). Mouse IgM Hs-14 Purified Hu, Ms F, ICC/IF, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

TER ATPase / VCP (C-term) antibody

Western blot analysis of VCP Antibody (C-term) (AP17823PU-N) in Jurkat, 293, MDA-MB231 cell line lysates (35ug/lane). VCP (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €380.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Immunohistochemistry (IHC) analyzes of VCP antibody (Cat.-No.: AP21142PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG1 58.13.3 Supernatant Hu, Rb, Rt, Xen WB
0.5 ml / €300.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG1 58.13.3 Purified Hu, Rb, Rt, Xen WB
50 µg / €310.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

TER ATPase / VCP Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €400.00
  GeneTex Inc.

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