TER ATPase / VCP antibody

Principal name

TER ATPase / VCP antibody

Alternative names for TER ATPase / VCP antibody

15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, Transitional endoplasmic reticulum ATPase

Selected pictures

TER ATPase / VCP antibody - SM5062 TER ATPase / VCP
SM5062 TER ATPase / VCP - Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (Cat.-No SM5062) shows staining in ViDr colon carcinoma cells.  F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (Cat.-No SM5062) at a 1/20-1/200 overnight at 4°C, washed with PBS and incubated with a Dylight-488 conjugated secondary antibody.
TER ATPase / VCP antibody - SM5062 TER ATPase / VCP
SM5062 TER ATPase / VCP - Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human brain tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/1000 with a mouse monoclonal antibody recognizing Anti-VCP (Cat.-No SM5062) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

SwissProt ID

P03974 (Pig), P23787 (Xenla), P46462 (Rat), P55072 (Human), Q01853 (Mouse), Q3ZBT1 (Bovin), Q7KN62 (Drome), Q7ZU99 (Danre)

Gene ID

7415 (VCP), 5327197 (IPR007158), 327197 (vcp)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Xen (Xenopus), Por (Porcine), Ze (Zebrafish), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E)

Background of TER ATPase / VCP antibody

P97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. 
Structure: Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
Interaction: VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP.
Bioactivity/Activities: VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
10736-1-AP

TER ATPase / VCP antibody

Immunohistochemical of  paraffin-embedded human colon cancer using 10736-1-AP(VCP antibody) at dilution of 1:50 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt E, IP, P, WB
0.15 ml / €285.00
  Proteintech Group, Inc.
60316-1-Ig

TER ATPase / VCP antibody

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig( VCP Antibody) at dilution of 1:1000 Mouse IgG1 2A4B10 Purified Hu, Ms E, P, WB
0.15 ml / €285.00
  Proteintech Group, Inc.
SM5062

TER ATPase / VCP antibody

Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (<em>Cat.-No</em> SM5062) shows staining in ViDr colon carcinoma cells.  F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (<em>Cat.-No</em> SM5062) at a 1/20-1/200 overnight at 4°C, washed with PBS and incubated with a Dylight-488 conjugated secondary antibody. Mouse IgG2a 5 Ascites Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH
GTX101089

TER ATPase / VCP antibody

VCP antibody detects VCP protein by Western blot analysis.A. 50 µg Rat brain lysate/extract7.5 % SDS-PAGEVCP antibody (GTX101089) dilution: 1:10000 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

Sample size available for 25µl / 119€
Buy any 2 samples and get a 15 % discount (read more…)

GTX113030

TER ATPase / VCP antibody

Sample (30 ug of whole cell lysate) A: Hela  B: Hep G2 (GTX27900)  C: Molt-4 (GTX27912)  D: Raji  7.5% SDS PAGE GTX113030 diluted at 1:1000 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

Sample size available for 25µl / 119€
Buy any 2 samples and get a 15 % discount (read more…)

GTX113099

TER ATPase / VCP antibody

Sample (30 ug of whole cell lysate)  A: NT2D1   B: U87-MG   C: MCF-7   7.5% SDS PAGE  GTX113099 diluted at 1:5000 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

Sample size available for 25µl / 119€
Buy any 2 samples and get a 15 % discount (read more…)

AM08335PU-M

TER ATPase / VCP antibody

Formalin-Fixed Paraffin-Embedded Human Prostate tissue stained with p97/VCP Antibody Cat.-No AM08335PU (Clone 4G9)  at 5 µg/ml after heat-induced antigen retrieval. Mouse IgG1 4G9 Aff - Purified Hu ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH
AM08335PU-T

TER ATPase / VCP antibody

Formalin-Fixed Paraffin-Embedded Human Prostate tissue stained with p97/VCP Antibody Cat.-No AM08335PU (Clone 4G9)  at 5 µg/ml after heat-induced antigen retrieval. Mouse IgG1 4G9 Aff - Purified Hu ICC/IF, IP, P, WB
25 µg / €220.00
  Acris Antibodies GmbH
AM31769PU-N

TER ATPase / VCP antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH
AP17823PU-N

TER ATPase / VCP (C-term) antibody

Western blot analysis of VCP Antibody (C-term) (AP17823PU-N) in Jurkat, 293, MDA-MB231 cell line lysates (35ug/lane). VCP (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €360.00
  Acris Antibodies GmbH
AP21142PU-N

TER ATPase / VCP antibody

Western blot (WB) analysis of VCP antibody (Cat.-No.: AP21142PU-N) in extracts from 3T3 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
BM5518

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG1 58.13.3 Supernatant Hu, Rb, Rt, Xen WB
0.5 ml / €290.00
  Acris Antibodies GmbH
BM5518P

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG1 58.13.3 Purified Hu, Rb, Rt, Xen WB
50 µg / €310.00
  Acris Antibodies GmbH
SM5062CP

TER ATPase / VCP Control Peptide

TER ATPase / VCP Purified
50 µg / €220.00
  Acris Antibodies GmbH
GTX59815

TER ATPase / VCP antibody

TER ATPase / VCP Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €400.00
  GeneTex Inc.
GTX62520

TER ATPase / VCP antibody

Immunohistochemical staining of paraffin-embedded human brain tissue using anti-VCP RabMAb (cat. #GTX62520). Rabbit IgG EPR3308 Supernatant Hu, Ms P, WB
0.1 ml / €420.00
  GeneTex Inc.
GTX62809

TER ATPase / VCP antibody

Immunofluorescent staining of HeLa cells using anti-VCP RabMAb. Rabbit IgG EPR3307(2) Supernatant Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €420.00
  GeneTex Inc.
GTX124821

TER ATPase / VCP antibody

Sample (30 ug of whole cell lysate)  A: zebrafish eye   7.5% SDS PAGE  GTX124821 diluted at 1:1000 Rabbit IgG Purified Ze P, WB
50 µl / €250.00
  GeneTex Inc.
GTX124821-200µl

TER ATPase / VCP antibody

Sample (30 ug of whole cell lysate)  A: zebrafish eye   7.5% SDS PAGE  GTX124821 diluted at 1:1000 Rabbit Purified Ze P, WB
0.2 ml / €440.00
  GeneTex Inc.
GTX128925

TER ATPase / VCP antibody

Vcp antibody detects Vcp protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos.  Vcp antibody (GTX128925) dilution: 1:200. Rabbit IgG Aff - Purified Ze
50 µl / €250.00
  GeneTex Inc.

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