TER ATPase / VCP antibody

Principal name

TER ATPase / VCP antibody

Alternative names for TER ATPase / VCP antibody

15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, Transitional endoplasmic reticulum ATPase

SwissProt ID

P03974 (Pig), P23787 (Xenla), P46462 (Rat), P55072 (Human), Q01853 (Mouse), Q3ZBT1 (Bovin), Q7KN62 (Drome), Q7ZU99 (Danre)

Gene ID

7415 (VCP), 5327197 (IPR007158), 327197 (vcp)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Xen (Xenopus), Por (Porcine), Ze (Zebrafish), Bov (Bovine), Chk (Chicken), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E)

Background of TER ATPase / VCP antibody

P97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. 
Structure: Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
Interaction: VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP.
Bioactivity/Activities: VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.

General readings

1. Peknicova J, Kyselova V, Buckiova D, Boubelik M.: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
2. Pavlasek J, Peknicova J, Ulcova-Gallova Z, Novakova P, Reischig J, Micanova Z, Rokyta Z.: Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction. Ceska Gynekol. 2004 Jul;69(4):306-11.
3. Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
4. Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J.: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
5. Peknicova J, Chladek D, Hozak P.: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis.
Am J Reprod Immunol. 2005 Jan;53(1):42-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TER ATPase / VCP antibody

Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9 Mouse IgG1 4G9 Aff - Purified Hu ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9 Mouse IgG1 4G9 Aff - Purified Hu ICC/IF, IP, P, WB
25 µg / €220.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

VCP antibody detects VCP protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VCP antibody (TA308570) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Western blot analysis of extracts from HeLa cells, using VCP (Ab-784) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Predicted band size: 90 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

WB Suggested Anti-VCP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TER ATPase / VCP antibody

Figure 1. Immunofluorescence analysis of 220 kDa intra-acrosomal protein in Acetone-permeabilized Human sperms using monoclonal antibody AM03029 Clone Hs-14 (normal spermiogram). Mouse IgM Hs-14 FITC Hu, Ms F
50 Tests / €340.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Immunofluorescence analysis of 220 kDa intra-acrosomal protein in acetone-permeabilized human sperms using monoclonal antibody Hs-14 (normal spermiogram). Mouse IgM Hs-14 Purified Hu, Ms F, ICC/IF, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

TER ATPase / VCP (C-term) antibody

Western blot analysis of VCP Antibody (C-term) (AP17823PU-N) in Jurkat, 293, MDA-MB231 cell line lysates (35ug/lane). VCP (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €380.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Immunohistochemistry (IHC) analyzes of VCP antibody (Cat.-No.: AP21142PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG1 58.13.3 Supernatant Hu, Rb, Rt, Xen WB
0.5 ml / €290.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG1 58.13.3 Purified Hu, Rb, Rt, Xen WB
50 µg / €310.00
  Acris Antibodies GmbH

TER ATPase / VCP antibody

Immunoperoxidase of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG2a 4A8 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TER ATPase / VCP antibody

TER ATPase / VCP Mouse IgG2a 2H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TER ATPase / VCP antibody

VCP monoclonal antibody (M18), clone 2B2. Western Blot analysis of VCP expression in A-431(Cat # L015V1 ). Mouse IgG1 2B2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TER ATPase / VCP antibody

TER ATPase / VCP Rabbit Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

TER ATPase / VCP pSer352 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with starved 24h, using VCP (Phospho-Ser352) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

TER ATPase / VCP pSer352 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with starved 24h, using VCP (Phospho-Ser352) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

TER ATPase / VCP antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with starved 24h, using VCP (Ab-352) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

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