TERF2 antibody

Principal name

TERF2 antibody

Alternative names for TERF2 antibody

TRBF2, TRF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

SwissProt ID

O35144 (Mouse), Q15554 (Human), Q9PU53 (Chick)

Gene ID

7014 (TERF2)

Available reactivities

Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Deer, Bov (Bovine), Can (Canine), African clawed frog

Available hosts

Rabbit, Mouse, Goat

Available applications

ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of TERF2 antibody

Human telomeres are composed of long sequences of TTAGGG repeats that form a nucleoprotein complex required for the stability, protection and replication of chromosome ends. One component of such human telomeres is the TTAGGG repeat binding factor 1 (TRF1), a ubiquitously expressed protein, related to the proto-oncogene Myb, that is present at telomeres throughout the cell cycle. Natural chromosomal ends are stabilized by proteins that bind duplex telomeric DNA repeats known as TTAGGG repeat Factors. Two independent studies isolated two proteins a 65kDa protein that bind to telomeric DNA and second open reading frame coding for 69kDa protein that binds to telomeric repeat DNA in vitro. These proteins are expressed in HeLa cells and together called TRF2. Affinity purified antibodies to TRF2 binds ro telomeres of intact human chromosomes (1). A stretch of 60 amino acids located at the C-terminal end of the protein is critical for the DNA binding. The region exhibit close homology with Myb repeats and is conserved in several proteins two of which are known to bind telomric repeat sequences. Both TRF1 and TRF2 are ubiquitously expressed, bound specifically to duplex TTAGGG repeats are localized to all human telomeres in metaphase chromosomes. TTAGGG repeat factor 2 (TRF2), is thought to protect telomere ends by remodeling them into T-loops.
Functionally TRF1 controls the telomere length and is proposed to be a telomerase inhibitor acting in cis to limit the telopere elongation of individual chromosome ends. TRF1 has similar architectural structure as of TRF2 both have a Myb motif and a large TRF1-related dimirization domain near its N-terminus. The dimerization domain of TRF1 and TRF2 do not interact suggesting that these two proteins exist as homodimmers. TRF2 specifically interacts with telomeric ss/ds DNA junctions and binding is sensitive to the sequence of the 3’, guanine-strand (G-strand) overhang and double-stranded DNA sequence at the junction. Association of TRF2 with DNA junctions hinders cleavage by exonuclease T (2). TRF1 and TRF2 differs at amino terminal end of the proteins, TRF2 being basic rather than acidic as in TRF1 protein (3). The TRF2 also plays a key role in protective activity of telomerase, prevent the end-to-end fusion of telomeres and are implicated in senescence in primary human cells due to shortening of the telomeres.

General readings

Ohbayashi T, Kishimoto T, Makino Y, Shimada M, Nakadai T, Aoki T, Kawata T, Niwa S, Tamura T.
Isolation of cDNA, chromosome mapping, and expression of the human TBP-like protein.
Biochem Biophys Res Commun. 1999 Feb 5;255(1):137-42.
PMID: 10082669

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TERF2 antibody

Western Blot: TRF2 Antibody - Analysis of TRF2 in A. HeLa WCE B. HeLa nuclear C. K562 D. HepG2 E. NIH/3T3 F. CHO G. PC12 H. Cos7 Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TERF2 (C-term) antibody

TA302827 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TERF2 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using TERF2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TERF2 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using TERF2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TERF2 antibody

Western Blot: TRF2 Antibody (4A794.15) [TA336371] - analysis of TRF2 in human Jurkat cell lysate using TRF2 antibody at 2 ug/ml.  Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Mouse IgG1 4A794.15 Purified Hu, Ms, Rt C, ChIP, E, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TERF2 antibody

Immunohistochemistry-Paraffin: TRF2 Antibody [TA336448] - Analysis of Human Tonsil using TRF2 antibody at 15 ug/ml. Goat Purified Hu, Ms C, ChIP, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TERF2 antibody

Western Blot: TRF2 Antibody (3H6B4) [TA337130] - Western blot analysis of Ntera2 (A) and K562 (B) cell lysate using TRF2 antibody at a concentration of 2 ug/ml. Mouse IgG1 3H6B4 Purified Hu C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TERF2 antibody

Western blot analysis of TERF2 in 293 cell lysate with TERF2 antibody at  1 ug/mL. Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TERF2 control peptide

TERF2
0.25 mg / €250.00
  Acris Antibodies GmbH

TERF2 (N-term) antibody

TERF2 Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TERF2 control peptide

TERF2
0.25 mg / €250.00
  Acris Antibodies GmbH

TERF2 antibody

TERF2 Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TERF2 control peptide

TERF2
0.25 mg / €250.00
  Acris Antibodies GmbH

TERF2 (C-term) antibody

TERF2 Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TERF2 antibody

Testis, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Can, Eq, Fe, Ft, GP, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb P
50 µg / €470.00
  Acris Antibodies GmbH

TERF2 antibody

Western Blot analysis of TERF2 expression in transfected 293T cell line (H00007014-T01) by TERF2 MaxPab polyclonal antibody.

Lane 1: TERF2 transfected lysate(27.61 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TERF2 antibody

TERF2 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TERF2 antibody

Immunoprecipitation ( IP ) / Western Blot ( WB ) Analysis of TERF2 in HL-60 cells : ( Lane 1 ) IP with mouse TERF2 monoclonal antibody, clone 4A794.15 ( Cat # MAB0074 ) . ( Lane 2 ) IP with control mouse IgG. ( Lane 3 ) IP with goat anti-TRF2. ( Lane 4 ) IP with pre-immune goat Ig. ( Lanes 1-4 ) WB with TERF2 monoclonal antibody, clone 4A794.15 ( Cat # MAB0074 ) . TRF2 is detected as an ~ 66 kD protein. Mouse IgG1 4A794.15 Deer, Hu, Ms, Rt C, E, F, ICC/IF, IP, P
0.1 mg / €470.00
  Abnova Taiwan Corp.

TERF2 antibody

Western blot analysis of TERF2 in HeLa nuclear extract using TERF2 polyclonal antibody ( Cat #PAB12598 ). Rabbit Hu ChIP, ICC/IF, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

TERF2 antibody

Transfected 293T; WB Suggested Anti-TERF2 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; TERF2 antibody - C-terminal region (ARP30228_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

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