TFAM / TCF6 antibody

Principal name

TFAM / TCF6 antibody

Alternative names for TFAM / TCF6 antibody

TCF6L2, MtTF1, TCF-6, mitochondrial Transcription factor A, Mitochondrial transcription factor 1, Transcription factor 6-like 2, Transcription factor 6

SwissProt ID

P40630 (Mouse), Q00059 (Human), Q0II87 (Bovin), Q5D144 (Pig), Q91ZW1 (Rat)

Gene ID

83474 (Tfam), 21780, 7019 (TFAM)

Available reactivities

Rt (Rat), Hu (Human), Bov (Bovine), Ms (Mouse), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of TFAM / TCF6 antibody

TFAM(Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

General readings

Sinha RA., et al. (2010) Biochem Biophys Res Commun. 397(3):548-52.
Aquilano K., et al. (2010) J Biol Chem. 285(28):21590-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TFAM / TCF6 antibody

Western Blot: mtTFA Antibody - Western blot analysis of mtTFA expression in HeLa whole cell lysate using anti-mtTFA. Mouse IgG 18G102B2E11 Purified Hu C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 (C-term) antibody

TFAM Antibody (C-term) (Cat. #TA324361) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 antibody

Western blot analysis of extracts of various celllines, using TFAM antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 antibody

Immunoperoxidase of purified MaxPab antibody to TFAM on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

TFAM / TCF6 antibody

Human; . Lanes: Lane 1 and 2: HepG2 cell lysate. Lane 3 and 4: Hep3B cell lysate. Lane 5 and 6: K562 cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: . Secondary Antibody Dilution: 1:1000. Gene Name: TFAM. Submitted by: Anonymous.; TFAM antibody - N-terminal region (ARP31400_P050) in Human cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por WB
50 µg / €350.00
  AVIVA Systems Biology

TFAM / TCF6 antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 antibody

WB Suggested Anti-TFAM Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 antibody

WB Suggested Anti-Tfam Antibody Titration: 0.2-1 ug/ml; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Bov, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 antibody

WB Suggested Anti-Tfam Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Muscle Rabbit IgG Purified Ms P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 antibody

WB Suggested Anti-Tfam Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Mouse Muscle Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFAM / TCF6 (C-term) antibody

Western blot of rat kidney lysate showing specific immunolabeling of the ~24k TFAM protein. Rabbit Ig Serum Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

TFAM / TCF6 antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu E, ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

TFAM / TCF6 antibody

Western Blot analysis of TFAM expression in transfected 293T cell line (H00007019-T02) by TFAM MaxPab polyclonal antibody.

Lane 1: TFAM transfected lysate(29.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

TFAM / TCF6 antibody

TFAM MaxPab rabbit polyclonal antibody. Western Blot analysis of TFAM expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TFAM / TCF6 antibody

Western blot analysis of Tfam expression in rat kidney tissue lysate. Rabbit Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TFAM / TCF6 antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

TFAM / TCF6 antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

TFAM / TCF6 antibody

Mouse NIH-3T3; WB Suggested Anti-TFAM Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: NIH/3T3 cell lysate; TFAM antibody - middle region (ARP36992_P050) in Mouse NIH-3T3 cells using Western Blot Rabbit Aff - Purified Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TFAM / TCF6 antibody

Mouse testicle; Tfam antibody - C-terminal region (ARP36993_P050) in Mouse testicle cells using Immunohistochemistry Rabbit Aff - Purified Bov, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

TFAM / TCF6 antibody

Mouse Muscle; WB Suggested Anti-Tfam Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Mouse Muscle; Tfam antibody - middle region (ARP58722_P050) in Mouse Muscle cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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