TFB2M antibody

Principal name

TFB2M antibody

Alternative names for TFB2M antibody

Dimethyladenosine transferase 2 mitochondrial, mtTFB2, NS5ATP5, S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2, Mitochondrial 12S rRNA dimethylase 2, Mitochondrial transcription factor B2, Hepatitis C virus NS5A-transactivated protein 5

SwissProt ID

Q32LD4 (Bovin), Q3TL26 (Mouse), Q5U2T7 (Rat), Q9H5Q4 (Human)

Gene ID

64216 (TFB2M), 216 (ALDH1A1), 15278 (Tfb2m)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TFB2M antibody

The transcription factors B1 (TFB1M; 607033) and B2 (TFB2M) are necessary for basal transcription of mammalian mitochondrial DNA. Human TFB1M and TFB2M are expressed ubiquitously and can each support promoter-specific mtDNA transcription in a pure recombinant in vitro system containing mitochondrial RNA polymerase and mitochondrial transcription factor A. TFB2M has been found to be at least 1 order of magnitude more active in promoting transcription than TFB1M. Both factors are highly homologous to bacterial rRNA dimethyltransferases, which suggests that an RNA-modifying enzyme has been recruited during evolution to function as a mitochondrial transcription factor.

General readings

Litonin,D.,et al. (2010) J. Biol. Chem. 285 (24), 18129-18133Norrbom,J.,et al. (2010) Acta Physiol (Oxf) 198 (1), 71-79.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TFB2M antibody

(0.5ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFB2M antibody

(0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFB2M antibody

WB Suggested Anti-TFB2M Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T Rabbit IgG Purified Eq, Hu, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFB2M antibody

WB Suggested Anti-TFB2M Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFB2M antibody

WB Suggested Anti-TFB2M Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFB2M (C-term) antibody

TFB2M Goat Aff - Purified Hu, Rt ICC/IF
0.1 mg / €340.00
  Acris Antibodies GmbH

TFB2M (C-term) antibody

TFB2M Antibody (C-term) (AP54226PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFB2M Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TFB2M antibody

TFB2M polyclonal antibody (A01), Lot # 060608JCS1 Western Blot analysis of TFB2M expression in U-2 OS ( Cat # L022V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TFB2M antibody

Western Blot analysis of TFB2M expression in transfected 293T cell line (H00064216-T01) by TFB2M MaxPab polyclonal antibody.

Lane 1: TFB2M transfected lysate(43.56 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TFB2M antibody

TFB2M monoclonal antibody (M01), clone 2E10. Western Blot analysis of TFB2M expression in human kidney. Mouse IgG1 2E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TFB2M antibody

TFB2M Goat Rt E
0.1 mg / €400.00
  Abnova Taiwan Corp.

TFB2M antibody

Human Intestine; TFB2M antibody - N-terminal region (ARP34471_T100) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Hu, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

TFB2M antibody

TFB2M Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TFB2M antibody

Transfected 293T; WB Suggested Anti-TFB2M Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; TFB2M antibody - C-terminal region (ARP39550_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

TFB2M antibody

Western Blot: TFB2M Antibody [NBP1-78283] - (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

TFB2M antibody

Immunohistochemistry-Paraffin: TFB2M Antibody [NBP1-82114] - Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TFB2M

TFB2M Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

TFB2M (20-396, His-tag)

TFB2M Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

TFB2M (20-396, His-tag)

TFB2M Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

TFB2M

TFB2M Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

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