TFE3 antibody

Principal name

TFE3 antibody

Alternative names for TFE3 antibody

Transcription factor E3

SwissProt ID

P19532 (Human), Q05B92 (Bovin), Q64092 (Mouse)

Gene ID

7030 (TFE3), 497030 (GTDC2), 5395 (PMS2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Goat, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot)

Background of TFE3 antibody

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB binds to the major late promoter of adenovirus and specifically associates with DNA as both a homodimer and a heterodimer with TFE3. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. Unlike TFE3, the expression of TFEC is largely restricted to fibroblasts, myoblasts, chondrosarcoma cells, and myeloma cells.

General readings

1. Henthorn PS, Stewart CC, Kadesch T, Puck JM.
The gene encoding human TFE3, a transcription factor that binds the immunoglobulin heavy-chain enhancer, maps to Xp11.22. Genomics. 1991 Oct;11(2):374-8. PMID: 1685140

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TFE3 antibody

Sample(30 ug whole cell lysate). A:Hep G2 . 7.5% SDS PAGE. TA308771 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TFE3 (N-term) antibody

(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

Western blot analysis of extracts from K562 cells and A549 cells, using TFE3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

Predicted band size: 62 kDa . Positive control: 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

TFE3 Antibody (N-term) (Cat. #TA324646) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

Immunohistochemistry staining of Paraffin Melanoma, ASPS tissue by TFE3 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear) Rabbit IgG MRQ-37 Purified Hu P
1 ml / €290.00
  OriGene Technologies, Inc.

TFE3 antibody

WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: NCI-H226 cell lysateTFE3 is supported by BioGPS gene expression data to be expressed in NCIH226 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

Western blot analysis on K562 cell lysate using TFE3 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

ELISA: Transcription factor E3 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

TFE3 antibody

TFE3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TFE3 antibody

TFE3 MaxPab rabbit polyclonal antibody. Western Blot analysis of TFE3 expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TFE3 antibody

TFE3 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

TFE3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TFE3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

TFE3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TFE3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

TFE3 antibody

Human MCF-7; WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; TFE3 antibody - middle region (ARP33439_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

TFE3 antibody

Human NCI-H226; WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: NCI-H226 cell lysate. TFE3 is supported by BioGPS gene expression data to be expressed in NCIH226.; TFE3 antibody - middle region (ARP33440_P050) in Human NCI-H226 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TFE3 antibody

Immunofluorescent analysis of TFE3 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TFE3 antibody

Immunofluorescent analysis of TFE3 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TFE3 antibody

Immunofluorescent analysis of TFE3 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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