TGFB1 antibody

Principal name

TGFB1 antibody

Alternative names for TGFB1 antibody

TGFB, Transforming growth factor beta-1, TGF-beta-1

SwissProt ID

O19011 (Horse), P01137 (Human), P04202 (Mouse), P07200 (Pig), P09531 (Chick), P17246 (Rat), P18341 (Bovin), Q9Z1Y6 (Cavpo)

Gene ID

7040 (TGFB1), 397078, 21803 (Tgfb1), 59086

Available reactivities

Ms (Mouse), Por (Porcine), Rt (Rat), Hu (Human), Sh (Sheep), Bov (Bovine), Can (Canine), GP (Guinea Pig), Hst (Hamster), Prim (Primate), Eq (Equine), Gt (Goat), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Neutralisation (NEUT)

Background of TGFB1 antibody

Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.1 TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.2 TGFbeta1 is also an important mediator of bone remodeling.3 TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.4 TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.5 TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.6 The standard product used in this kit is recombinant TGFβ1 with the molecular mass of 25KDa.

General readings

Assoian RK, Komoriya A, et al. (1983). J Biol Chem. 258(11):7155-60.Choy L, et al. (1998). J Biol Chem. 273(47):31455-62.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TGFB1 antibody

Immunohistochemistry (IHC) analyzes of TGF-beta1 antibody (Cat.-No.: AP06350PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TGFB1 antibody

Western blot result of the antibody used at 2μg/mL to detect platelet derived TGF-β at 12μg/lane (lane 2) 6μg/lane (lane 3), 3 μg/lane (lane 4) and 1μg/mL (lane 5) with 4- chlorol-napthol as substrate. The bottom band is the 12.5KD TGF-β monomer, the mid-band is the 25KD TGF-β dimer and the up band is TGF-β with signal peptide. Lane 1 is molecular weight markers. Mouse IgG1 TB21 Azide Free Hu, Sh C, E, FN, P, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

TGFB1 antibody

Anti-TGFB1 mouse monoclonal antibody (TA506585) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TGFB1(RC200973). Mouse IgG2a OTI1E12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGFB1 (N-term) antibody

TGFB1 Antibody (N-term) (AP54230PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TGFB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TGFB1 antibody

Western blot analysis of A549 cell lysates with anti-TGF beta1 antibody (1:500). Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TGFB1 antibody

Western blot analysis of extracts from HepG2 cells, using TGF ß1 antibody (#TA313319).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TGFB1 (286-293) antibody

WB analysis using Anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at RT for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L)  diluted 1:2,500. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TGFB1 antibody

Predicted band size: 44 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TGFB1 antibody

Predicted cell location: Secreted,Cytoplasm. Positive control: Human liver and gastric cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using TGFB1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TGFB1 antibody

WB Suggested Anti-Tgfb1 Antibody Titration: 1.25 ug/ml; Positive Control: Mouse Lymphnode Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TGFB1 antibody

Western blot analysis of extracts from HepG2 cells, using TGF beta Antibody. The lane on the left is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TGFB1 antibody

Anti-TGFB1 mouse monoclonal antibody (TA506583) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TGFB1(RC200973). Mouse IgG2a OTI3B6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGFB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGFB1 (RC200973, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFB1. Mouse IgG2a OTI2B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGFB1 antibody

TGFB1 Mouse IgG2a Azide Free Hu E, P, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

TGFB1 antibody

Western blot analysis using TGF beta-1 antibody Cat.-No AM06127SU-N against truncated TGF beta1 recombinant protein. Mouse IgG2a GE 4.90 Ascites Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

TGFB1 antibody

TGFB1 Mouse IgG1 TB21 Aff - Purified Hu C, E, F, NEUT, P, WB
1.0 ml / €470.00
  Acris Antibodies GmbH

TGFB1 antibody

TGFB1 Mouse IgG1 TB21 PE Hu F
100 Tests / €940.00
  Acris Antibodies GmbH

TGFB1 (286-293) antibody

Figure 1. AP07828PU-N TGFB1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 5 µg/ml, followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TGFB1 antibody

TGFB1 Mouse IgG1 2C5 Azide Free Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

TGFB1 antibody

TGFB1 Mouse IgG1 2C6 Azide Free Hu C, E, FN, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

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