TGIF1 antibody

Principal name

TGIF1 antibody

Alternative names for TGIF1 antibody

TGIF, 5'-TG-3'-interacting factor 1, Homeobox protein TGIF1

SwissProt ID

P70284 (Mouse), Q15583 (Human)

Gene ID

7050 (TGIF1), 21815 (Tgif1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of TGIF1 antibody

TGIF1 is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptiol co-repressor of SMAD2 and may participate in the transmission of nuclear sigls during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain.The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptiol co-repressor of SMAD2 and may participate in the transmission of nuclear sigls during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Altertive splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TGIF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802284) Mouse IgG1 OTI1B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 antibody

  Rabbit Polyclonal Anti-TGIF1  
WB Suggested Anti-TGIF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

TGIF1 antibody

  Rabbit Polyclonal TGIF Antibody  
ELISA: TGIF Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI1G5  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 (RC201549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGIF1. Mouse IgG1 OTI1G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI4D10 (formerly 4D10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 (RC201549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGIF1. Mouse IgG1 OTI4D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 (RC201549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGIF1. Mouse IgG1 OTI1H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 (RC201549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGIF1. Mouse IgG1 OTI2F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 (RC201549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGIF1. Mouse IgG1 OTI2E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 antibody

  TGIF1 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TGIF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802371) Mouse IgG1 OTI1A6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TGIF1 (Center) antibody

Western blot analysis of anti-TGIF1 Antibody (Center) (AP14007PU-N) in Y79 cell line lysates (35 µg/lane). TGIF1 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TGIF1 (Center) antibody

Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center A160) (AP54234PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TGIF1 (Center) antibody

Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223) (AP54235PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TGIF1 - human gene knockout kit via CRISPR

TGIF1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TGIF1 - human gene knockout kit via CRISPR

TGIF1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TGIF1 - human gene knockout kit via CRISPR

TGIF1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TGIF1 - human gene knockout kit via CRISPR

TGIF1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TGIF1 (transcript variant 3)

  Recombinant protein of human TGFB-induced factor homeobox 1 (TGIF1), transcript variant 3  
TGIF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

TGIF1 (full length, N-term HIS tag, transcript variant 3)

  Recombinant protein of human TGFB-induced factor homeobox 1 (TGIF1), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
TGIF1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

TGIF1 (full length, N-term HIS tag)

  Recombinant protein of human TGFB-induced factor homeobox 1 (TGIF1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
TGIF1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

TGIF1 (full length, N-term HIS tag, transcript variant 6)

  Purified recombinant protein of Human TGFB-induced factor homeobox 1 (TGIF1), transcript variant 6, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
TGIF1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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