THEMIS antibody

Principal name

THEMIS antibody

Alternative names for THEMIS antibody

Protein Themis, Grb2-associating protein, Gasp, Protein thylex, Thymocyte-expressed molecule involved in selection

Gene ID

210757 (Themis), 387357 (THEMIS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of THEMIS antibody

Thymocyte-expressed molecule involved in selection (THEMIS) is a protein coding gene that plays a regulatory role in positive and negative T-cell selection during late thymocyte development (1). THEMIS is located on human chromosome 6q22.33. It is a member of a new metazoan gene family and is required for completion of thymocyte positive selection (2). THEMIS contains a tandem repeat of a unique globular domain which includes cysteine motif that defines a family consisting of five uncharacterized vertebrae proteins that have orthologs in most animal species (2). THEMIS appears to be related to TCR signaling and, because it is quickly phosphorylated after TCR stimulation, it is necessary for optimal TCR-driven calcium mobilization as well as being necessary for activation of the ErK-Kinase (3).
Recently it was reported that THEMIS is essential for the transition of CD4+ CD8+ double-positive (DP) cells to MHC class I-restricted CD4- CD8+ and MHC class II-restricted CD4+ CD8- single positive (SP) cells (4) In agreement with these findings THEMIS deficient thymocytes showed defective positive selection which caused fewer thymocytes to reach maturation (5). In these mice, CD4SP thymocyte maturation was more severely affected than CD8SP due to the fact that sustained signaling is essential for CD4SP thymocyte development (6). A large percentage of THEMIS-deficient cells had a CD4(+)CD25(+)Foxp3(t) regulatory and CD62L(10)CD44(hi) memory phenotypes than compared to wild type of T cells (3). THEMIS is expressed in thymocytes between the Pre-T cell antigen receptor (pre-TCR) and positive selection checkpoints.
THEMIS is approximately a 77kDa protein (641 amino acids).

7 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

THEMIS antibody

(0.3ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

THEMIS Control Peptide

THEMIS
0.1 mg / €250.00
  Acris Antibodies GmbH

THEMIS antibody

THEMIS Rabbit Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

THEMIS (C-term) antibody

THEMIS Antibody (C-term) (AP54239PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of THEMIS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

THEMIS antibody

THEMIS Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

THEMIS antibody

Immunohistochemistry-Paraffin: THEMIS Antibody [NBP1-90802] - Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells. Rabbit Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

THEMIS overexpression lysate

THEMIS overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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