Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Thiamine pyrophosphokinase 1 (TPK1) antibody
This gene encodes a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Nosaka K., et al. (2001) Biochim. Biophys. Acta. 1517:293-297Zhao R., et al. (2004) Biochim. Biophys. Acta. 1517:320-322
Immunohistochemical of paraffin-embedded human colon cancer using 10942-1-AP(TPK1 antibody) at dilution of 1:10 (under 10x lens)
Human Placenta; WB Suggested Anti-TPK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; TPK1 antibody - N-terminal region (ARP55386_P050) in Human Placenta cells using Western Blot
Formalin-fixed and paraffin-embedded human testis tissue reacted with TPK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of TPK1 (arrow) using rabbit polyclonal TPK1 Antibody (N-term) (AP54344PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPK1 gene.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.