Thioredoxin / TRX1 antibody

Principal name

Thioredoxin / TRX1 antibody

Alternative names for Thioredoxin / TRX1 antibody

TXN, TRDX, TRX, ATL-derived factor, ADF, SASP, Surface-associated sulphydryl protein

SwissProt ID

O97680 (Bovin), P08628 (Rabit), P08629 (Chick), P10599 (Human), P10639 (Mouse), P11232 (Rat), P50413 (Sheep)

Gene ID

7295 (TXN)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), E. coli (Escherichia coli), Bov (Bovine), Por (Porcine), Rb (Rabbit), Can (Canine), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Functional assay (FN), Flow Cytometry (F), Dot blot (Dot)

Background of Thioredoxin / TRX1 antibody

Thioredoxins (Trx) are small, multi functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase (PDI) and a number of transcription factors such as p53, NFkB and AP1.Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.TRX family; composed of two groups: Group I, which includes proteins that exclusively encode a TRX domain; and Group II, which are composed of fusion proteins of TRX and additional domains. Group I TRX is a small ancient protein that alter the redox state of target proteins via the reversible oxidation of an active site dithiol, present in a CXXC motif, partially exposed at the protein's surface. TRX reduces protein disulfide bonds, resulting in a disulfide bond at its active site. Oxidized TRX is converted to the active form by TRX reductase, using reducing equivalents derived from either NADPH or ferredoxins. By altering their redox state, TRX regulates the functions of at least 30 target proteins, some of which are enzymes and transcription factors. It also plays an important role in the defense against oxidative stress by directly reducing hydrogen peroxide and certain radicals, and by serving as a reductant for peroxiredoxins. At least two major types of functional TRXs have been reported in most organisms; in eukaryotes, they are located in the cytoplasm and the mitochondria. Higher plants contain more types (at least 20 TRX genes have been detected in the genome of Arabidopsis thaliana), two of which (types f amd m) are located in the same compartment, the chloroplast. Also included in the alignment are TRX-like domains which show sequence homology to TRX but do not contain the redox active CXXC motif. Group II proteins, in addition to either a redox active TRX or a TRX-like domain, also contain additional domains, which may or may not possess homology to known proteins.

General readings

Perkins AV et al. Immunocytochemical localization of thioredoxin in human trophoblast and decidua. Placenta 16:635-42 (1995). PubMed PMID: 8577662

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Thioredoxin / TRX1 antibody

Immunofluorescence of monoclonal antibody to TXN on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2A7 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

Predicted band size: 12 kDa. Positive control: Hela cell and human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Hela cell tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thioredoxin / TRX1 antibody

Predicted band size: 12 kDa. Positive control: Hela and MCF7 cell, human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Hela cell tissue lysate Lane2: MCF7 cell tissue lysate Lane3: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thioredoxin / TRX1 antibody

Predicted band size: 12 kDa. Positive control: A172 and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: A172 cell lysate lysate Lane2: Hela cell lysate lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thioredoxin / TRX1 antibody

Predicted band size: 12 kDa. Positive control: A172 and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: A172 cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thioredoxin / TRX1 antibody

TXN Antibody (C-term) (Cat. #TA325133) western blot analysis in A549,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the TXN antibody detected the TXN protein (arrow). Rabbit IgG RB42816 Purified Bov, Hu, Ms, Por, Rb, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Thioredoxin / TRX1 antibody

Host: Rabbit; Target Name: TXN; Sample Tissue: MCF7 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thioredoxin / TRX1 (TRX-tag) antibody

Western blot analysis using Trx antibody Cat.-No AM06168SU-N against various fusion protein with Trx tag. Mouse IgG1 1H6H6 Ascites Hu WB
0.1 ml / €300.00
  Acris Antibodies GmbH

Thioredoxin / TRX1 antibody

Thioredoxin / TRX1 Mouse IgG1 2C9 Azide Free E. coli WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Thioredoxin / TRX1 antibody

Western blot analysis of human Thioredoxin expression in Raji (1), HeLa (2), WR19L (3) and PC12 (4) using AM26612AF-N. Mouse IgG2a 2E3 Azide Free Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Thioredoxin / TRX1 (N-term) antibody

Western blot analysis of anti-TrX antibody  (Cat.#AP11336PU-N) in HL-60 cell line lysate. TrX(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Thioredoxin / TRX1 antibody

Human Lung (formalin-fixed, paraffin-embedded) stained with TXN antibody AP22618PU-N at 1/200 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Thioredoxin / TRX1 antibody

Thioredoxin / TRX1 Mouse IgG1 2B1 Purified Hu C, E, P, WB
0.2 mg / €670.00
  Acris Antibodies GmbH

Thioredoxin / TRX1 antibody

Thioredoxin / TRX1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

TXN polyclonal antibody (A02), Lot # 051003JC01 Western Blot analysis of TXN expression in MES-SA/Dx5 ( Cat # L021V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

Western Blot analysis of TXN expression in transfected 293T cell line (H00007295-T03) by TXN MaxPab polyclonal antibody.

Lane 1: TXN transfected lysate(11.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

Immunofluorescence of monoclonal antibody to TXN on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 6C10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

Immunoblot analysis of cell lysates. Lane 1 : Hela cell lysate and lane 2 : Jurkat cell lysate. Mouse IgG2b 3A1 Hu C, E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

Immunoprecipitation analysis of 293T cell lysates. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with TXN monoclonal antibody, clone 8A1 ( Cat # MAB0721 ). Mouse IgG1 8A1 Hu E, IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

Thioredoxin / TRX1 antibody

Western blot analysis of TXN polyclonal antibody ( Cat # PAB2465 ) in HL-60 cell line lysate. TXN ( arrow ) was detected using the purified polyclonal antibody. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

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