Thrombin receptor / F2R antibody

Principal name

Thrombin receptor / F2R antibody

Alternative names for Thrombin receptor / F2R antibody

Proteinase-activated receptor 1, PAR-1, PAR1, CF2R, Coagulation factor II receptor

SwissProt ID

A7YY44 (Bovin), P25116 (Human), P26824 (Rat), P30558 (Mouse), P47749 (Xenla)

Gene ID

2149 (F2R)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), African clawed frog, GP (Guinea Pig), Mky (Monkey), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of Thrombin receptor / F2R antibody

Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR-1 - 4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their me. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-termil domain. This results in the creation of a new N-termil tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several sigl transduction pathways. Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin. PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others. The intramolecular ture of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termition of PAR sigling. Indeed, following receptor activation, there is rapid phosphorylation of the C-termil end of the receptor, followed by receptor interlization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby â??disarmingâ? the PAR. PAR-1 sigls through several G proteins including Gaq, Gai, and Ga12/13, resulting in the activation of several transduction pathways including intracellular Ca2+ mobilization, Rho and Rac sigling, and MAPK activation. PAR-1 is expressed in several cell types including platelets, leukocytes, vascular endothelial cells, gastrointestil epithelial cells, myocytes, and neurons. The best studied physiological function of PAR-1 is its involvement in the coagulation cascade. Thrombin, the preeminent ligand of PAR-1, activates the receptor on the surface of platelets, hence inducing platelet aggregation, granular secretion, and procoagulant activity. PAR-1 also plays a crucial role in vascular ontogenesis. Accordingly, PAR-1 knockout mice show bleeding at multiple sites and usually die at mid-gestation. PAR-1 also plays important roles in tumor growth and metastasis. PAR-1 is upregulated in several human cancers as are several proteases such as plasmin and matrix metalloproteases (MMPs) that act as PAR-1 ligands, thereby creating an autocrine loop. PAR-1 activation in cancer cells transmits mitogenic sigls through the activation of the erk1/2 pathway and is involved in tumor spread via its pro-angiogenic activity.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Thrombin receptor / F2R antibody

Western blot analysis of human promyelocytic leukemia HL-60 (lanes 1 and 4), human T-cell leukemia Jurkat (lanes 2 and 5), and chronic myelogenous leukemia K562 (lanes 3 and 6) cell line lysates: 1-3. Anti-Human Protease-Activated Receptor-1 (extracellular) antibody, (1:200). 4-6. Anti-Human Protease-Activated Receptor-1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

AP15817PU ATAP2 antibody staining of Human Pancreas. Rabbit Purified Hu, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Thrombin receptor / F2R antibody

AP15817PU ATAP2 antibody staining of Human Pancreas. Rabbit Purified Hu, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

Thrombin receptor / F2R antibody

AP15817PU ATAP2 antibody staining of Human Pancreas. Rabbit Purified Hu, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

Thrombin receptor / F2R antibody

TA305911 (0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Western Blot: Thrombin Receptor Antibody (N2-11) - Western blot analysis of Thrombin Receptor expression in human testis. Mouse IgG1 N2-11 Purified Hu, Ms C, E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Western blot analysis of extracts from HeLa cells, treated with Nocodazole (1ug/ml, 18hours), using Thrombin Receptor antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Western blot analysis of extracts from Jurkat cells, using PAR1 (Cleaved-Ser42) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using F2R antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Predicted band size: 47 kDa . Positive control: K562 cell, human lymphoma and fetal kidney tissue lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

WB Suggested Anti-F2R Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysateF2R is supported by BioGPS gene expression data to be expressed in HeLa Rabbit IgG Purified GP, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R (N-term, extracel. dom.) antibody

Anti-F2R / Thrombin Receptor / PAR1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Mky, Prim P
50 µg / €379.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Anti-Thrombin Receptor antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

WB Suggested Anti-F2R Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: U937 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Western blot analysis on HeLa cell lysate using Thrombin Receptor Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Thrombin receptor / F2R Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombin receptor / F2R antibody

Thrombin receptor / F2R Mouse IgG1 B825M Purified Hu F, IP, WB
0.2 mg / €650.00
  Acris Antibodies GmbH

Thrombin receptor / F2R antibody

Western blot analysis using PAR1 antibody Cat.-No AM06270SU-N against truncated GST-PAR1 recombinant protein (Lane 1). Mouse IgG1 6A7H10 Ascites Hu WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Thrombin receptor / F2R antibody

Western blot (WB) analysis of PAR1 antibody (Cat.-No.: AP06630PU-N) in extracts from HeLa cells treated with Nocodazole 1µg/ml 18 hours. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Thrombin receptor / F2R antibody

Western blot (WB) analyzes of Thrombin R antibody (Cat.-No.: AP21049PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu WB
0.1 mg / €390.00
  Acris Antibodies GmbH

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