Thrombopoietin antibody

Principal name

Thrombopoietin antibody

Alternative names for Thrombopoietin antibody

THPO, MGDF, C-mpl ligand, Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, Megakaryocyte growth and development factor

SwissProt ID

P40225 (Human), P40226 (Mouse), P42705 (Canfa), P42706 (Pig), P49745 (Rat)

Gene ID

7066 (THPO), 21832 (Thpo), 103690065 (LOC103690065), 81811

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Thrombopoietin antibody

Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

General readings

Blood. 2009 Aug 20;114(8):1655-7. Epub 2009 Jun 24.
Ann Hepatol. 2008 Jul-Sep;7(3):235-44.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Thrombopoietin antibody

Anti-Thrombopoietin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

Western blot analysis using recombinant human TPO. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

Predicted band size: 38 kDa . Positive control: 293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

To detect hTPO by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (TA328460) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

Western blot analysis using THPO antibody Cat.-No AM06758PU-N against human THPO (AA: 250-303) recombinant protein (Expected MW is 30 kDa) Mouse IgG2a 1B11 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Thrombopoietin (C-term) antibody

Western blot analysis of anti-THPO(C-term) Pab in K562 cell line lysates (35ug/lane). THPO(arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Aff - Purified Hu E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.

Lane 1: THPO transfected lysate(38.39 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.

Lane 1: THPO transfected lysate(37.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Western blot analysis of THPO polyclonal antibody ( Cat # PAB2956 ) in K-562 cell line lysates ( 35 µg/lane ) . THPO ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Thpo polyclonal antibody ( Cat # PAB13838 ) ( 1 µg/ml ).
Secondary antibody : ABC Alkaline Phosphatase Kit.
Lane 1 : 100 ng mouse Thpo ( non-reduced ).
Lane 2 : 100 ng mouse Thpo ( reduced ). Sheep Ms E, WB
1 mg / €640.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58734_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Thrombopoietin antibody

Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58735_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Thrombopoietin antibody

Western blot analysis of Thrombopoietin expression in HepG2 (A); mouse liver (B); rat liver (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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