Thrombopoietin antibody

Principal name

Thrombopoietin antibody

Alternative names for Thrombopoietin antibody

THPO, MGDF, C-mpl ligand, Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, Megakaryocyte growth and development factor

SwissProt ID

P40225 (Human), P40226 (Mouse), P42705 (Canfa), P42706 (Pig), P49745 (Rat)

Gene ID

7066 (THPO), 21832 (Thpo), 81811

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Thrombopoietin antibody

Thrombopoietin (TPO), a hormone predominately expressed in liver and bone marrow stromal cells, is the principal regulator of proliferation and differentiation of megakaryoctytes, and is the major cytokine involved in platelet production. TPO has also been shown to pay a role in production of primitive pluripotent stem cells and progenitor cells, such as erythroid and myeloid progenitor cells. Human TPO cDNA encodes a 353 amino acid precursor protein that is cleaved upon secretion from the cell. Mature human TPO is a 95kDa glycoprotein that comprises two domains, a receptor binding domain that shares homology with erythropoietin, and a heavily O- and N-linked glycosylated region at the carboxy terminus. Potential clinical applications for TPO include the in-vivo expansion of platelet numbers following thrombocytopenia inducing effects of chemotherapy. TPO also shows promise in the ex-vivo expansion of autologous haematopoietic progenitor cells prior to re-infusion into patients with conditions such as multiple myeloma and NHL following myeloabalative chemotherapy. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Solberg (2005) Current Hematology Reports 4(6):423-8 and Fishley & Alexander (2004) Growth Factors. 22(3):151-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Thrombopoietin antibody

Anti-Thrombopoietin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

Western blot analysis using recombinant human TPO. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

Predicted band size: 38 kDa . Positive control: 293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

To detect hTPO by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (TA328460) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Thrombopoietin antibody

Western blot analysis using THPO antibody Cat.-No AM06758PU-N against human THPO (AA: 250-303) recombinant protein (Expected MW is 30 kDa) Mouse IgG2a 1B11 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Thrombopoietin (C-term) antibody

Western blot analysis of anti-THPO(C-term) Pab in K562 cell line lysates (35ug/lane). THPO(arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Thrombopoietin Goat Aff - Purified Hu E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Thrombopoietin antibody

Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.

Lane 1: THPO transfected lysate(38.39 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.

Lane 1: THPO transfected lysate(37.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Western blot analysis of THPO polyclonal antibody ( Cat # PAB2956 ) in K-562 cell line lysates ( 35 µg/lane ) . THPO ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Thpo polyclonal antibody ( Cat # PAB13838 ) ( 1 µg/ml ).
Secondary antibody : ABC Alkaline Phosphatase Kit.
Lane 1 : 100 ng mouse Thpo ( non-reduced ).
Lane 2 : 100 ng mouse Thpo ( reduced ). Sheep Ms E, WB
1 mg / €640.00
  Abnova Taiwan Corp.

Thrombopoietin antibody

Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58734_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Thrombopoietin antibody

Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58735_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Thrombopoietin antibody

Western blot analysis of Thrombopoietin expression in HepG2 (A); mouse liver (B); rat liver (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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