Thrombospondin-1 (THBS1) antibody

Principal name

Thrombospondin-1 (THBS1) antibody

Alternative names for Thrombospondin-1 (THBS1) antibody

TSP, TSP1

SwissProt ID

P07996 (Human), P35441 (Mouse), P35448 (Xenla), Q28178 (Bovin)

Gene ID

7057 (THBS1), 55901 (THSD1)

Available reactivities

Bov (Bovine), Can (Canine), Eq (Equine), Fe (Feline), Hu (Human), Mam (Mammalia), Ms (Mouse), Por (Porcine), Rt (Rat), Chk (Chicken), Sh (Sheep), Gt (Goat), Mky (Monkey), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Functional assay (FN), ELISA (detection) (E(detection)), Frozen Sections (C), Immunodiffusion (ID), Enzyme Immunoassay (E)

Background of Thrombospondin-1 (THBS1) antibody

Heparin efficiently inhibits the binding of Thrombospondin to TSP. Hyaluronic acid and chondroitin sulfate show no inhibition at low concentration and only partially inhibit over the concentration range at which heparin abolishes the binding. Immunoprecipitates a 25 kDa peptide (heparinbinding domain) from chymotryptic digests of TSP that is not disulfide bonded to any other region of the TSP molecule. It inhibits the hemagglutination of trypsinized, glutaraldehyde-fixed human erythrocytes by purified TSP. Also inhibits the agglutination of fixed, activated platelets by TSP. It does not inhibit either thrombin- or A23187-induced aggregation of washed, live platelets. It does not bind to reduced and alkylated TSP or TSP transferred to nitrocellulose membrane after SDS-PAGE. It
blocks the binding of TSP to sulfatides at low concentrations. It shows no cross-reaction with fibronectin, fibrinogen, and von Willebrand factor.

General readings

1. DeFreitas MF; Yoshida CK; Frazier WA; Mendrick DL; Kypta RM; Reichardt LF. Identification of integrin alpha 3 beta 1 as a neuronal thrombospondin receptor mediating neurite outgrowth. Neuron, 1995 Aug, 15(2):333-43.
2. RayChaudhury A; Frazier WA; D'Amore PA. Comparison of normal and tumorigenic endothelial cells: differences in thrombospondin production and responses to transforming growth factor-beta. Journal of Cell Science, 1994 Jan, 107 ( Pt 1):39-46.
3. Tolsma SS; Volpert OV; Good DJ; Frazier WA; Polverini PJ; Bouck N. Peptides derived from two separate domains of the matrix protein thrombospondin-1 have anti-angiogenic activity.
Journal of Cell Biology, 1993 Jul, 122(2):497-511.
4. Frazier WA. Thrombospondins. Current Opinion in Cell Biology, 1991 Oct, 3(5):792-9.
5. Prater CA; Plotkin J; Jaye D; Frazier WA. The properdin-like type I repeats of human thrombospondin contain a cell attachment site. Journal of Cell Biology, 1991 Mar, 112(5):1031-40.
6. Good DJ; Polverini PJ; Rastinejad F; Le Beau MM; Lemons RS; Frazier WA; Bouck NP. A tumor suppressor-dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin. Proceedings of the National Academy of Sciences of the United States of America, 1990 Sep, 87(17):6624-8.
7. Frazier WA. Thrombospondin: a modular adhesive glycoprotein of platelets and nucleated cells. Journal of Cell Biology, 1987 Aug, 105(2):625-32.
8. Santoro SA; Frazier WA. Isolation and characterization of thrombospondin. Methods in Enzymology, 1987, 144:438-46.
9.Prater CA, et. al. J Cell Biol, 1991, 112(5):1031-40.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Thrombospondin-1 (THBS1) antibody

Immunohistochemical staining of formalin-fixed paraffin-embedded tonsil showing membrane staining with anti-THBS1 antibody diluted at 1:100. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Thrombospondin-1 (THBS1) (N-term) antibody

Western blot analysis of THBS1 Antibody (N-term) (Cat. #TA325040) in MDA-MB231 cell line lysates (35ug/lane). THBS1 (arrow) was detected using the purified Pab. Rabbit Hu F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Thrombospondin-1 (THBS1) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human THBS1 with C-terminal MYC tag probed with TA326717 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with TA326717 (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombospondin-1 (THBS1) (N-term) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human THBS1 with C-terminal MYC tag probed with TA326718 (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with TA326718 (0.1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Thrombospondin-1 (THBS1) (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human Spleen stained with Thrombospondin-1 Antibody Cat.-No AP23121PU-N at 3.75 µg/ml after heat-induced antigen retrieval. Goat Purified Can, Eq, Gt, Hu, Mky, Ms, Por, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

Thrombospondin-1 (THBS1) antibody

Thrombospondin-1 (THBS1) Rabbit Serum Hu ID, IP
1 ml / €430.00
  Acris Antibodies GmbH

Thrombospondin-1 (THBS1) antibody

Formalin Fixed Paraffin Embedded Human placenta stained with Thrombospodin Antibody Cat.-No DM315 (Clone A6.1). Mouse IgG1 A6.1 Purified Bov, Can, Eq, Fe, Hu, Mam, Ms, Por, Rt ICC/IF, IP, P, WB
1 ml / €690.00
  Acris Antibodies GmbH

Thrombospondin-1 (THBS1) antibody

Formalin Fixed Paraffin Embedded Human placenta stained with Thrombospodin Antibody Cat.-No DM315 (Clone A6.1). Mouse IgG1 A6.1 Purified Mam ICC/IF, P, WB
0.5 ml / €450.00
  Acris Antibodies GmbH

Thrombospondin-1 (THBS1) antibody

Thrombospondin-1 (THBS1) Rabbit Aff - Purified Hu WB
0.1 ml / €220.00
  GeneTex Inc.

Thrombospondin-1 (THBS1) antibody

Immunofluorescence of purified MaxPab antibody to THBS1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Thrombospondin-1 (THBS1) antibody

Western Blot: Thrombospondin Antibody (A4.1) [NB600-554] - Western Blot of TSP from Human Cells in a Non-reducing Gel. Mouse IgM A4.1 Purified Bov, Chk, Hu, Ms, Rt F, ICC/IF, WB
0.5 ml / €400.00
  Novus Biologicals Inc.

Thrombospondin-1 (THBS1) antibody

Thrombospondin-1 (THBS1) Mouse IgG1 Bov, Hu, Ms, Por, Rt C, F, ICC/IF, IP, WB
50 µg / €440.00
  Novus Biologicals Inc.

Thrombospondin-1 (THBS1) antibody

Thrombospondin-1 (THBS1) Goat Aff - Purified Hu, Ms, Rt P
50 µg / €480.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1) Human Purified > 95 % pure by SDS-PAGE Platelets
25 µg / €280.00
  Acris Antibodies GmbH

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1)
10 µg / €470.00
  GeneTex Inc.

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1) Human
10 µg / €450.00
  Abnova Taiwan Corp.

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1) Human
25 µg / €380.00
  Abnova Taiwan Corp.

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1) Human
0.1 mg / €910.00
  Abnova Taiwan Corp.

Thrombospondin-1 (THBS1)

Thrombospondin-1 (THBS1) Human
0.1 mg / €910.00
  Abnova Taiwan Corp.

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