TICAM1 / TRIF antibody

Principal name

TICAM1 / TRIF antibody

Alternative names for TICAM1 / TRIF antibody

TICAM-1, PRVTIRB, TIR domain-containing adapter molecule 1, Proline-rich vinculin and TIR domain-containing protein B, Toll-interleukin 1 receptor domain-containing adapter protein inducing interferon-beta, Putative NF-kappa-B-activating protein 502H

SwissProt ID

Q4JF29 (Bovin), Q80UF7 (Mouse), Q8IUC6 (Human)

Gene ID

148022 (TICAM1), 106759 (Ticam1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TICAM1 / TRIF antibody

During the development of immunity, Toll like receptors (TLRs) are activated by adjuvants and reveal molecular mechanisms by which different ligands are recognized by the same receptor in different shapes. Among various ligands that activated TLR are deoxycytidylate-phosphoate-deoxy guanylate (CpGp)DNA, which activate TLR and monophopshoryl lipid A, derived from bacterial endotoxin, to activate TLR 4. The recognition of lipidA by TLR 4 requires MD-2 which binds to extracellular domain on TLR 4. This TLR4-MD2 complex then reorganizes the TIR domain of the TLR4 enabling the recruitment of 4 adapter molecules, MyD88, TRIF (also known as TICAM1) and TRAM (also known as TICAM2). Interaction of these adapter molecules initiate the signal transduction cascade that led the production and secretion of molecules that regulate immune response as well as enhance the cell surface expression of molecules on specialized immune cells (dentritic cells) that enhance binding of T cells. The mechanism of immune response diversification though not fully understood, is partly explained by the way TLR-MD-2 complexes alter the TIR domain via the adapter molecules. The variations in the signaling pathways differ either TRAM or TRIF are activated and or Mal and MyD88 pathways are activated. The TRIF and TRAM activation results in INF-b induced activation of T cells that modulate the immune response and Mal and MyD88 activation resulted in NFkb and cytokine release that led to inflammation and harm to the host (1).
The transcript for mouse TRIF are clustered into 33,409 “transcriptional units” of these transcriptional units which exhibit high degree of alternate splicing about 4000 are new protein-coding and 11665 are new non coding regions. TRIF gene is one of the many new transcriptional unit new protein. TRIF is a highly conserved protein in various species ranging in 209 -279 amino acids. TRIF contains a RING domain of 40-60 amino acids that bind 2 atoms of zinc by cross brace motif is involved in protein-protein interactions that is necessary for signal transduction, development and replication (2).

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TICAM1 / TRIF antibody

Western blot analysis of TRIF in human lung cell lysates with TRIF antibody at (A) 2 and (B) 4 ug/ml. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM1 / TRIF antibody

TICAM1 Antibody (N-term) (Cat. #TA325013) western blot analysis in Ramos cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the TICAM1 antibody detected the TICAM1 protein (arrow). Rabbit IgG Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TICAM1 / TRIF antibody

Western Blot: TRIF Antibody [TA336579] - analysis of TRIF in mouse A) intestine, B) spleen and C) RAW lysate using TRIF antibody at 1:1000 dilution. Rabbit Purified Hu, Ms, Rt ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

TICAM1 / TRIF antibody

Western Blot: TRIF Antibody - Analysis in human lung cell lysates at (A) 2 and (B) 4 ug/ml. Rabbit Purified Hu C, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

TICAM1 / TRIF antibody

TA348961 (2 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM1 / TRIF antibody

Total cell lysates from Raji cells (lane 1) and mouse spleen cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TICAM-1 (clone 1H4B01) antibodies. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse 1H4B01 Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM1 / TRIF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TICAM1 (RC217522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TICAM1. Mouse IgG1 OTI1G7 Purified Hu, Mky WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

TICAM1 / TRIF antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TICAM1 / TRIF Control Peptide

TICAM1 / TRIF
0.1 mg / €250.00
  Acris Antibodies GmbH

TICAM1 / TRIF antibody

TICAM1 / TRIF Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TICAM1 / TRIF Control Peptide

TICAM1 / TRIF
50 µg / €160.00
  Acris Antibodies GmbH

TICAM1 / TRIF antibody

Western blot analysis of Ticam1 in mouse spleen lysate. Using Ticam1 polyclonal antibody ( Cat # PAB0317 ) at 1 : 1000 dilution. Rabbit Hu, Ms WB
0.2 ml / €470.00
  Abnova Taiwan Corp.

TICAM1 / TRIF antibody

Western blot analysis of TICAM1 expression in human lung tissue with TICAM1 polyclonal antibody at 4 µg/ml. Rabbit Hu C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

TICAM1 / TRIF antibody

Immunohistochemistry of TICAM1 in human lung tissue with TICAM1 polyclonal antibody ( Cat # PAB12905 ) at 10 µg/ml. Rabbit Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TICAM1 / TRIF antibody

TICAM1 / TRIF Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

TICAM1 / TRIF antibody

TICAM1 / TRIF Rabbit IgG Aff - Purified Ms WB
0.1 mg / €290.00
  Boster Immunoleader

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TICAM1 / TRIF

TICAM1 / TRIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TICAM1 / TRIF

TICAM1 / TRIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

TICAM1 overexpression lysate

TICAM1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

19 Item(s)

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