TICAM2 / TRAM antibody

Principal name

TICAM2 / TRAM antibody

Alternative names for TICAM2 / TRAM antibody

TICAM-2, TIRAP3, TIRP, TIR domain-containing adapter molecule 2, TRIF-related adapter molecule, Toll/interleukin-1 receptor domain-containing protein, Toll-like receptor adaptor protein 3,Putative NF-kappa-B-activating protein 502

SwissProt ID

Q2LGB7 (Bovin), Q86XR7 (Human), Q8BJQ4 (Mouse)

Gene ID

100302736 (TMED7-TICAM2), 353376 (TICAM2), 225471 (Ticam2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Dot blot (Dot), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TICAM2 / TRAM antibody

The mammalian host defense system is essentially regulated by Toll-like receptor (TLR) family. At least 10 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM). Most of the TLRs are dependent on the expression of adapter molecules MyD88 for all their function, the TL3 and TLR 4 are unique in their ability to mediate both MyD88-dependent and independent reposes (1). The MyD88 independent pathway involves induction of a DC maturation and induction of type 1 interferon (IFN-B). A new TIRF-related adapter molecule (TRAM) also known as TICAM 2, which like TRIF, activates IRF3 and IRF7. TRAM appears to be restricted to LPS activation (TLR4) pathway, compared to TRIF which play a role in both TLR3 and TL R4 pathways leading to IRF gene expression. The TRAM may involve TLR4-specfic signaling resulting in gene activation profile not shared by TLR3 pathways (2).
The TRAM/ICAM 2 protein is located on chromosome 5q22. The TRAM/ICAM 2 is a 32-34 kDa protein (235 aa) comprise of a large Toll-interleukin 1 resistance (TIR) domain with a short (70 aa) N-terminal Ser/Thr rich domain (TIR motif) and a 20 amino acid C-terminal end. The unique N-terminal end of TRAM/ICAM 2 did not resemble those of ICAM 1 however, the TIR and the C-terminal end are highly conserved in human and mouse TRAM/ICAM 2. There are subtle differences in the TIR domain of the TRAM/TICAM 2 and other known adapters like TIRAP, MyD88, however, the N-terminal domain of TRAM/TICAM 2 shared tandemly arranged basic amino acids and serine with that of TIRAP (3).

General readings

1. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003;24:286 - 9.
2 ) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003;3:466 - 77.
3 ) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003;21:335 - 76.
4 ) Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002;20:197 - 216.
5. UniProtKB/TrEMBL entry Q86XR7, http://expasy.org/uniprot/Q86XR7, Accessed January 30, 2007

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TICAM2 / TRAM (C-term) antibody

  Rabbit Polyclonal TIRP Antibody  
Western blot analysis of TIRP in PC-3 cell lysate with TIRP antibody at 1 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM antibody

  Rabbit Polyclonal TIRP Antibody  
Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM antibody

  Rabbit Polyclonal Anti-TMED7-TICAM2 Antibody  
Host: Rabbit; Target Name: TIRAP3; Sample Tissue: RPMI-8226 whole cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM antibody

  Mouse Monoclonal anti-TICAM-2 (TRAM) Antibody  
Western blot analysis of THP-1 (lane 1) and HEK293 (lane 2) cells using anti-TICAM-2 (TRAM) antibody (9G1B30). Mouse 9G1B30 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM (C-term) antibody

Figure 1. Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 µg/ml. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

TICAM2 / TRAM antibody

Western blot analysis of TIRP in PC-3 cell lysate with TIRP antibody at 1 µg/ml. Rabbit IgG Purified Hu C, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

TICAM2 / TRAM (C-term) antibody

Figure 1. Staining TIRP in Brain, cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TICAM2 - human gene knockout kit via CRISPR

TICAM2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TICAM2 - human gene knockout kit via CRISPR

TICAM2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TICAM2 - human gene knockout kit via CRISPR

TICAM2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TICAM2 - human gene knockout kit via CRISPR

TICAM2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ticam2 - mouse gene knockout kit via CRISPR

Ticam2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ticam2 - mouse gene knockout kit via CRISPR

Ticam2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ticam2 - mouse gene knockout kit via CRISPR

Ticam2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ticam2 - mouse gene knockout kit via CRISPR

Ticam2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TICAM2 / TRAM

  Recombinant protein of human toll-like receptor adaptor molecule 2 (TICAM2)  
TICAM2 / TRAM Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

TICAM2 / TRAM (1-235, His-tag)

TICAM2 / TRAM Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

TICAM2 / TRAM (1-235, His-tag)

TICAM2 / TRAM Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TICAM2 (mGFP-tagged) - Human toll-like receptor adaptor molecule 2 (TICAM2), 200 uL, >10^7 TU/mL

Lenti ORF particles, TICAM2 (mGFP-tagged) - Human toll-like receptor adaptor molecule 2 (TICAM2), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, TICAM2 (Myc-DDK tagged) - Human toll-like receptor adaptor molecule 2 (TICAM2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, TICAM2 (Myc-DDK tagged) - Human toll-like receptor adaptor molecule 2 (TICAM2) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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