TICAM2 / TRAM antibody

Principal name

TICAM2 / TRAM antibody

Alternative names for TICAM2 / TRAM antibody

TICAM-2, TIRAP3, TIRP, TIR domain-containing adapter molecule 2, TRIF-related adapter molecule, Toll/interleukin-1 receptor domain-containing protein, Toll-like receptor adaptor protein 3,Putative NF-kappa-B-activating protein 502

SwissProt ID

Q2LGB7 (Bovin), Q86XR7 (Human), Q8BJQ4 (Mouse)

Gene ID

100302736 (TMED7-TICAM2), 353376 (TICAM2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Dot blot (Dot), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TICAM2 / TRAM antibody

The mammalian host defense system is essentially regulated by Toll-like receptor (TLR) family. At least 10 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM). Most of the TLRs are dependent on the expression of adapter molecules MyD88 for all their function, the TL3 and TLR 4 are unique in their ability to mediate both MyD88-dependent and independent reposes (1). The MyD88 independent pathway involves induction of a DC maturation and induction of type 1 interferon (IFN-B). A new TIRF-related adapter molecule (TRAM) also known as TICAM 2, which like TRIF, activates IRF3 and IRF7. TRAM appears to be restricted to LPS activation (TLR4) pathway, compared to TRIF which play a role in both TLR3 and TL R4 pathways leading to IRF gene expression. The TRAM may involve TLR4-specfic signaling resulting in gene activation profile not shared by TLR3 pathways (2).
The TRAM/ICAM 2 protein is located on chromosome 5q22. The TRAM/ICAM 2 is a 32-34 kDa protein (235 aa) comprise of a large Toll-interleukin 1 resistance (TIR) domain with a short (70 aa) N-terminal Ser/Thr rich domain (TIR motif) and a 20 amino acid C-terminal end. The unique N-terminal end of TRAM/ICAM 2 did not resemble those of ICAM 1 however, the TIR and the C-terminal end are highly conserved in human and mouse TRAM/ICAM 2. There are subtle differences in the TIR domain of the TRAM/TICAM 2 and other known adapters like TIRAP, MyD88, however, the N-terminal domain of TRAM/TICAM 2 shared tandemly arranged basic amino acids and serine with that of TIRAP (3).

General readings

1. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003;24:286 - 9.
2 ) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003;3:466 - 77.
3 ) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003;21:335 - 76.
4 ) Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002;20:197 - 216.
5. UniProtKB/TrEMBL entry Q86XR7, http://expasy.org/uniprot/Q86XR7, Accessed January 30, 2007

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TICAM2 / TRAM (C-term) antibody

Western blot analysis of TIRP in PC-3 cell lysate with TIRP antibody at 1 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM antibody

Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM antibody

Host: Rabbit; Target Name: TIRAP3; Sample Tissue: RPMI-8226 whole cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

TICAM2 / TRAM (C-term) antibody

Figure 1. Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 µg/ml. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TICAM2 / TRAM antibody

Western blot analysis of TIRP in PC-3 cell lysate with TIRP antibody at 1 µg/ml. Rabbit IgG Purified Hu C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TICAM2 / TRAM antibody

Figure 1. Staining TIRP in Brain, cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TICAM2 / TRAM control peptide

TICAM2 / TRAM
0.1 mg / €270.00
  Acris Antibodies GmbH

TICAM2 / TRAM pSer16 antibody

TICAM2 / TRAM Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

TICAM2 / TRAM Control Peptide

TICAM2 / TRAM
0.1 mg / €250.00
  Acris Antibodies GmbH

TICAM2 / TRAM antibody

Western Blot analysis using  TRAM amtibody (AP10356PU-N at 1/500 dilution). Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TICAM2 / TRAM Control Peptide

TICAM2 / TRAM
50 µg / €160.00
  Acris Antibodies GmbH

TICAM2 / TRAM Control Peptide

TICAM2 / TRAM
50 µg / €160.00
  Acris Antibodies GmbH

TICAM2 / TRAM antibody

TICAM2 / TRAM Rabbit IgG Aff - Purified Fe, Hu, Mky, Por Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TICAM2 / TRAM antibody

Western Blot analysis of TICAM2 expression in transfected 293T cell line (H00353376-T02) by TICAM2 MaxPab polyclonal antibody.

Lane 1: TICAM2 transfected lysate(26.90 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TICAM2 / TRAM antibody

TICAM2 / TRAM Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TICAM2 / TRAM antibody

Western Blot analysis of TICAM2 expression in transfected 293T cell line (H00353376-T02) by TICAM2 MaxPab polyclonal antibody.

Lane 1: TICAM2 transfected lysate(26.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

TICAM2 / TRAM antibody

Western Blot analysis of TICAM2 expression in transfected 293T cell line (H00353376-T02) by TICAM2 MaxPab polyclonal antibody.

Lane 1: TICAM2 transfected lysate(26.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TICAM2 / TRAM antibody

Western blot analysis of TICAM2 expression in PC-3 cell lysate with TICAM2 polyclonal antibody ( Cat # PAB9740 ) at 1 µg/ml. Rabbit Hu C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

TICAM2 / TRAM antibody

Detection of TICAM2 ( human ) in HEK 293T transfected cells by TICAM2 polyclonal antibody ( Cat # PAB13794 ). 10 µg per lane of total cell extracts from mock transfected HEK 293T cells ( lane 1 ) or HEK 293T cells transfected with an expression vector encoding VSV-tagged human TICAM2 ( lane 2 ). Rabbit Hu WB
50 µg / €470.00
  Abnova Taiwan Corp.

TICAM2 / TRAM antibody

Immunohistochemistry-Paraffin: TICAM2 Antibody [NBP1-76616] - TIRP in Raji cells with TIRP antibody at 10 ug/mL._x000d_ Rabbit Purified Hu E, ICC/IF, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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