TIF1-alpha / TRIM24 antibody

Principal name

TIF1-alpha / TRIM24 antibody

Alternative names for TIF1-alpha / TRIM24 antibody

TIF1 alpha, TIF1A, TIF1, RNF82, RING finger protein 82, Transcription intermediary factor 1-alpha, Tripartite motif-containing protein 24

SwissProt ID

O15164 (Human)

Gene ID

8805 (TRIM24), 828805 (ACO2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection))

Background of TIF1-alpha / TRIM24 antibody

**Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognize native protein in any application. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

General readings

Thenot S, Henriquet C, Rochefort H, Cavailles V.
Differential interaction of nuclear receptors with the putative human transcriptional coactivator hTIF1. J Biol Chem. 1997 May 2;272(18):12062-8. PMID: 9115274

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIF1-alpha / TRIM24 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRIM24 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802797) Mouse IgG1 OTI2D9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

Immunoperoxidase of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml] Mouse IgG3 2F2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TIF1-alpha / TRIM24 (C-term) antibody

(4ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Bov, Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-alpha / TRIM24 antibody

Western blot analysis of extracts from COLO cells, using TRIM24 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-alpha / TRIM24 antibody

Western blot analysis of TRIM24 in EL4 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-alpha / TRIM24 antibody

TRIM24 Antibody ( N-term) (Cat. #TA324630) western blot analysis in U-937,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the TRIM24 antibody detected the TRIM24 protein (arrow). Rabbit IgG Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TIF1-alpha / TRIM24 antibody

Western blot analysis on Jurkat and K562 cell lysate using TRIM24 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-alpha / TRIM24 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24. Mouse IgG1 OTI3A8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24. Mouse IgG1 OTI2H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24. Mouse IgG1 OTI1D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24. Mouse IgG1 OTI1D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24. Mouse IgG1 OTI1A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) to amino acids 706-1016 of human TRIM24 (NP_003843). Mouse IgG1 OTI1G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIF1-alpha / TRIM24 antibody

Immunohistochemistry (IHC) analyzes of TIF1alpha antibody (Cat.-No.: AP510PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TIF1-alpha / TRIM24 (C-term) antibody

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TIF1-alpha / TRIM24 antibody

Western Blot analysis of TRIM24 expression in transfected 293T cell line (H00008805-T02) by TRIM24 MaxPab polyclonal antibody.

Lane 1: TRIM24 transfected lysate(116.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TIF1-alpha / TRIM24 antibody

TIF1-alpha / TRIM24 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

TIF1-alpha / TRIM24 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRIM24 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

TIF1-alpha / TRIM24 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRIM24 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

TIF1-alpha / TRIM24 antibody

Human HeLa; WB Suggested Anti-TRIM24 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate. TRIM24 is supported by BioGPS gene expression data to be expressed in HeLa.; TRIM24 antibody - middle region (ARP34449_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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