TIF1-beta / TRIM28 antibody

Principal name

TIF1-beta / TRIM28 antibody

Alternative names for TIF1-beta / TRIM28 antibody

TIF1 beta, TIF1B, KAP1, KAP-1, KRIP1, KRIP-1, RNF96, RING finger protein 96, Transcription intermediary factor 1-beta, Tripartite motif-containing protein 28, KRAB-associated protein 1, Nuclear corepressor KAP-1, KRAB-interacting protein 1

SwissProt ID

O08629 (Rat), Q13263 (Human), Q62318 (Mouse)

Gene ID

10155 (TRIM28), 21849 (Trim28), 116698

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Bt (Bat), Eq (Equine), Mky (Monkey), Prim (Primate), Rb (Rabbit), Bear

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ChIP assay (ChIP)

Background of TIF1-beta / TRIM28 antibody

Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1 beta is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
Structure: An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus. 
Distribution: Nucleus.
Function: TIF1 beta acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins. 
Interaction: TIF1 beta was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1 alpha or TIF1 gamma.

General readings

1. Frietze S, O'Geen H, Blahnik KR, Jin VX, Farnham PJ. ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes. PLoS One. 2010 Dec 8;5(12):e15082.
PMID: 21170338.
2. Torres-Padilla ME, Zernicka-Goetz M. Role of TIF1alpha as a modulator of embryonic transcription in the mouse zygote. J Cell Biol. 2006 Jul 31;174(3):329-38. PMID: 16880268

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIF1-beta / TRIM28 (C-term) antibody

  Goat Polyclonal Antibody against TRIM28  
TA302849 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms ChIP, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 pSer473 antibody

  Rabbit Polyclonal anti-TIF1ß Phospho (Ser473) Antibody  
Hela cell extracts untreated (lane 1) and treated with 10 Gy radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β Phospho (Ser473) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Mouse Monoclonal KAP1 / TIF1 beta Antibody  
Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa. Mouse IgG1 4E1-D12-F8 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 4E6 Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 antibody

  Rabbit Polyclonal TRIM28 Antibody  
Western blot analysis of TRIM28 in human testis tissue lysate with TRIM28 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Anti-TRIM28 Rabbit Polyclonal Antibody  
Predicted band size: 89 kDa . Positive control: Jurkat, T98G, COS7 and Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3,4: Jurkat, T98G, COS7 and Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Rabbit polyclonal TRIM28 Antibody (N-term)  
TRIM28 Antibody (N-term) (Cat. #TA324442) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow). Rabbit IgG Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Goat Anti-KAP1 Antibody  
TA326731 (1µg/ml) staining HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Mouse Monoclonal anti-TIF1ß Antibody  
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 20A1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Mouse Monoclonal anti-TIF1ß (KAP-1, TRIM28) Phospho (Ser473) Antibody  
Hela cell extracts untreated (lane 1) and treated with UV radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1ß phosphorylated (Ser473) antibody (clone 11G10SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse 11G10SC Purified Hu ICC/IF, WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

  Mouse Monoclonal anti-TIF1ß (KAP-1, TRIM28) Antibody  
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1szlig; antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse 20A1 Purified Hu ChIP, ICC/IF, WB
25 µg / €248.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1D11 Purified Hu, Ms, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 (823-835) antibody

Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bt, Can, Eq, Hu, Mky, Ms, Prim, Rb, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Trim28 - mouse gene knockout kit via CRISPR

Trim28 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Trim28 - mouse gene knockout kit via CRISPR

Trim28 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Trim28 - mouse gene knockout kit via CRISPR

Trim28 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Trim28 - mouse gene knockout kit via CRISPR

Trim28 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TIF1-beta / TRIM28

  Recombinant protein of human tripartite motif-containing 28 (TRIM28)  
TIF1-beta / TRIM28 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 (366-802, His-tag)

TIF1-beta / TRIM28 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 (366-802, His-tag)

TIF1-beta / TRIM28 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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