TIF1-beta / TRIM28 antibody

Principal name

TIF1-beta / TRIM28 antibody

Alternative names for TIF1-beta / TRIM28 antibody

TIF1 beta, TIF1B, KAP1, KAP-1, KRIP1, KRIP-1, RNF96, RING finger protein 96, Transcription intermediary factor 1-beta, Tripartite motif-containing protein 28, KRAB-associated protein 1, Nuclear corepressor KAP-1, KRAB-interacting protein 1

SwissProt ID

O08629 (Rat), Q13263 (Human), Q62318 (Mouse)

Gene ID

10155 (TRIM28)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Broad, Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ChIP assay (ChIP), Flow Cytometry (F)

Background of TIF1-beta / TRIM28 antibody

Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1 beta is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
Structure: An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus. 
Distribution: Nucleus.
Function: TIF1 beta acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins. 
Interaction: TIF1 beta was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1 alpha or TIF1 gamma.

General readings

1. Frietze S, O'Geen H, Blahnik KR, Jin VX, Farnham PJ. ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes. PLoS One. 2010 Dec 8;5(12):e15082.
PMID: 21170338.
2. Torres-Padilla ME, Zernicka-Goetz M. Role of TIF1alpha as a modulator of embryonic transcription in the mouse zygote. J Cell Biol. 2006 Jul 31;174(3):329-38. PMID: 16880268

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIF1-beta / TRIM28 (C-term) antibody

TA302849 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms ChIP, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 pSer473 antibody

Hela cell extracts untreated (lane 1) and treated with 10 Gy radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β Phospho (Ser473) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 (C-term) antibody

TA302849 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms ChIP, E, ICC/IF, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 antibody

Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa. Mouse IgG1 4E1-D12-F8 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 4E6 Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 antibody

Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2b 4E6 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TIF1-beta / TRIM28 antibody

Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Mouse IgG2a 1D11 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TIF1-beta / TRIM28 antibody

Western blot analysis of TRIM28 in human testis tissue lysate with TRIM28 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

Predicted band size: 89 kDa . Positive control: Jurkat, T98G, COS7 and Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3,4: Jurkat, T98G, COS7 and Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

TRIM28 Antibody (N-term) (Cat. #TA324442) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow). Rabbit IgG Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

TA326731 (1µg/ml) staining HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 20A1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIF1-beta / TRIM28 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1D11 Purified Hu, Ms, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 antibody

HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 20A1 Purified Hu WB
25 µg / €210.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 (823-835) antibody

Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bt, Can, Eq, Hu, Mky, Ms, Prim, Rb, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TIF1-beta / TRIM28 antibody

TRIM28 polyclonal antibody ( Cat # PAB6320 ) staining ( 0.5 µg/ml ) of HepG2 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms C, E, ICC/IF, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TIF1-beta / TRIM28 antibody

Human MCF-7; WB Suggested Anti-TRIM28 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate. TRIM28 is supported by BioGPS gene expression data to be expressed in MCF7.; TRIM28 antibody - N-terminal region (ARP31460_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TIF1-beta / TRIM28 antibody

Human Lung; . Rabbit Anti-TRIM28 antibody. . Catalog Number:. ARP32677_P050 . Paraffin Embedded Tissue: Human Lung cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; TRIM28 antibody (ARP32677_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

TIF1-beta / TRIM28 antibody

Human Heart; . Rabbit Anti-TRIM28 antibody. . Catalog Number:. ARP38681_T100 . Paraffin Embedded Tissue: Human Heart cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; TRIM28 antibody (ARP38681_T100) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

TIF1-beta / TRIM28 antibody

TIF1-beta / TRIM28 Rabbit IgG Aff - Purified Hu WB
50 µg / €680.00
  Funakoshi Co., Ltd.

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