TIGAR antibody

Principal name

TIGAR antibody

Alternative names for TIGAR antibody

C12orf5, Probable fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator

SwissProt ID

Q1JQA7 (Bovin), Q8BZA9 (Mouse), Q9NQ88 (Human)

Gene ID

57103 (C12orf5), 319801 (9630033F20Rik)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Enzyme Immunoassay (E)

Background of TIGAR antibody

TIGAR (TP53-induced glycolysis and apoptosis regulator), a member of phosphoglycerate mutase family, is a target gene of p53 and it regulates glucose metabolism in cancer cells by acting as a fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate (Fru-2,6-P2 ) and Fru-1,6-P2 leading to decreased Fru-2,6-P2 levels with subsequent reduction in PFK1 (phosphofructosekise-1) activity as well as negative regulation of glycolysis. TIGAR also reduces cellular ROS through its translocation to the mitochondria where it interacts with HK2 leading to enhanced HK2 activity, regulation of mitochondrial membrane potential, and decreased mitochondrial ROS which ultimately protects the cells from D damage-induced apoptosis. Moreover, TIGAR mediated negative regulation of glycolysis leads to redirection of glycolytic metabolic intermediates towards pentose phosphate pathway's oxidative branch that follows increased cellular DPH production, ROS scavenging by GSH and thus a lower sensitivity of cells to oxidative stress-associated apoptosis, including that induced by p53. Because of TIGAR's anti-apoptotic/protective role, it has been proposed to implicate in cell-cycle arrest and D repair effector response in cells that suffer from repairable dose of genotoxic/cellular insult.

General readings

Bensaad K., et al. (2009) EMBO J. 28(19):3015-26.
Hasegawa H., et al. (2009) Leukemia. 23(11):2090-101.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIGAR (Center) antibody

  Rabbit Polyclonal TIGAR Antibody  
Western blot analysis of TIGAR in EL4 cell lysate with TIGAR antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIGAR (Center) antibody

  Rabbit Polyclonal TIGAR Antibody  
Immunofluorescence of TIGAR in Human Brain cells with TIGAR antibody at 20 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIGAR antibody

  Anti-C12orf5 Rabbit Polyclonal Antibody  
Predicted cell location: Nucleus,Cytoplasm. Positive control: Human cervical and breast cancer tissue . Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using C12orf5 antibody at dilution 1/80, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIGAR antibody

  Rabbit Polyclonal TIGAR/C12orf5 Antibody  
Western Blot: TIGAR Antibody [TA336787] - Analysis of TIGAR in HeLa whole cell extracts. Rabbit Purified Hu, Ms C, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIGAR antibody

  Rabbit Polyclonal Anti-C12orf5 Antibody  
TIGAR Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIGAR antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TIGAR antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

9630033F20Rik - mouse gene knockout kit via CRISPR

9630033F20Rik - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

9630033F20Rik - mouse gene knockout kit via CRISPR

9630033F20Rik - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

9630033F20Rik - mouse gene knockout kit via CRISPR

9630033F20Rik - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

9630033F20Rik - mouse gene knockout kit via CRISPR

9630033F20Rik - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

C12orf5 - human gene knockout kit via CRISPR

C12orf5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

C12orf5 - human gene knockout kit via CRISPR

C12orf5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

C12orf5 - human gene knockout kit via CRISPR

C12orf5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

C12orf5 - human gene knockout kit via CRISPR

C12orf5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TIGAR

  Recombinant protein of human chromosome 12 open reading frame 5 (C12orf5)  
TIGAR Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

TIGAR

  Purified recombinant protein of Human chromosome 12 open reading frame 5 (C12orf5).  
TIGAR Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 25 µg / €199.00

Special Price: 25 µg / €139.00

  OriGene Technologies, Inc.

TIGAR

  Purified recombinant protein of Human chromosome 12 open reading frame 5 (C12orf5).  
TIGAR Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 25 µg / €199.00

Special Price: 25 µg / €139.00

  OriGene Technologies, Inc.

TIGAR (1-270, His-tag)

TIGAR Human Purified > 90 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

TIGAR (1-270, His-tag)

TIGAR Human Purified > 90 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

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