TIMD3 / HAVCR2 antibody

Principal name

TIMD3 / HAVCR2 antibody

Alternative names for TIMD3 / HAVCR2 antibody

TIMD-3, TIM3, TIM-3, T-cell membrane protein 3, HAVCR-2, Hepatitis A virus cellular receptor 2

SwissProt ID

P0C0K5 (Rat), Q8TDQ0 (Human), Q8VIM0 (Mouse)

Gene ID

84868 (HAVCR2), 171285 (Havcr2)

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine)

Available hosts

Rat, Goat, Rabbit, Mouse

Available applications

Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P)

Background of TIMD3 / HAVCR2 antibody

The 8B.2C12 monoclonal antibody reacts with mouse Tim-3, a Th1-specific cell surface protein. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. Tim-3 is expressed selectively by differentiated CD4+Th1 and CD8+Tc1 cells, but is absent on CD4+Th2 and CD8+Tc2 cells. Other hematopoietic cell types, including nave T cells, B cells, macrophages and dendritic cells, do not express Tim-3, at least at the protein level. Tim-3 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, Tim-3 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed Tim-3 after immunization. In this model, in vivo administration of 8B.2C12 resulted in a hyperacute and atypical disease phenotype. It is postulated that the engagement of Tim-3 during T cell activation results in the expansion and activation of macrophages and increased severity of an autoimmune disease. The Tim gene family may have an important role in the regulation of autoimmunity and allergies. The 8B.2C12 antibody binds to the Tim-3 BALB/c allele. Reactivity to the C57/Bl6 is significantly weaker than BALB/c.

General readings

Meyers JH, Sabatos CA, Chakravarti S, Kuchroo VK. The TIM gene family regulates autoimmune and allergic diseases.Trends Mol Med. 2005 Aug;11(8):362-9. Review. PMID: 16002337

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIMD3 / HAVCR2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HAVCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807034)(1:150) Mouse IgG1 OTI2E2 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIMD3 / HAVCR2 antibody

TA302624 (0.3µg/ml) staining of human tonsil lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

Western blot analysis of Tim-3 expression in Jurkat cell lysate. Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

Staining of non-transfected (left) and mouse TIM3-transfected (right) CHO cells with 0.125 ug of Rat IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Mouse TIM3 Purified (filled histogram) followed by Anti-Rat IgG FITC .  Total cells were used for analysis. Rat 8B.2C12 Purified Ms F, IP
50 µg / €120.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat RMT3-23 Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

Western blot analysis of extracts of various cell lines, using HAVCR2 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

Western Blot: TIM-3 Antibody [TA336355] - Analysis of TIM-3 in A) mouse and B) rat kidney cell lysate using this antibody at 2 ug/ml. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

WB Suggested Anti-HAVCR2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat IgG2a 25F.1D6 Biotin Ms F
0.1 mg / €290.00
  Acris Antibodies GmbH

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat IgG2a 25F.1D6 FITC Ms C, F
0.1 mg / €290.00
  Acris Antibodies GmbH

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat IgG2a 25F.1D6 Purified Ms C, F
0.25 mg / €350.00
  Acris Antibodies GmbH

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat IgG2a 25F.1D6 PE Ms F, WB
50 µg / €320.00
  Acris Antibodies GmbH

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat IgG2 6F46 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

TIMD3 / HAVCR2 antibody

HEK293 overexpressing HAVCR2 and probed with HAVCR2 polyclonal antibody ( Cat # PAB6962 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TIMD3 / HAVCR2 antibody

Western blot analysis of HAVCR2 expression in Jurkat cell lysate. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TIMD3 / HAVCR2 antibody

Human OVCAR-3; WB Suggested Anti-HAVCR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: OVCAR-3 cell lysate; HAVCR2 antibody - N-terminal region (ARP45550_P050) in Human OVCAR-3 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TIMD3 / HAVCR2 (C-term) antibody

TIMD3 / HAVCR2 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TIMD3 / HAVCR2 antibody

Mouse Tim-3 transfected cells stained with purified RMT3-23, followed by anti-rat IgG FITC Rat IgG2a RMT3-23 Aff - Purified Ms F
50 µg / €320.00
  Novus Biologicals Inc.

TIMD3 / HAVCR2 antibody

Western Blot: HAVCR2 Antibody [NB600-821] - NB600-821 (0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

TIMD3 / HAVCR2 antibody

TIMD3 / HAVCR2 Rat IgG2 RM0135-6F46 Purified Ms WB
0.1 mg / €450.00
  Novus Biologicals Inc.

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