Timeless (Tim) antibody

Principal name

Timeless (Tim) antibody

Alternative names for Timeless (Tim) antibody

Timeless1, Tim1, Protein timeless homolog

SwissProt ID

O17482 (Drovi), O44431 (Drohy), P49021 (Drome), Q9R1X4 (Mouse), Q9UNS1 (Human), Q9Z2Y1 (Rat)

Gene ID

8914 (TIMELESS), 21853 (Timeless)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Dros (Drosophila), African clawed frog, Eq (Equine), Por (Porcine), Sh (Sheep), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Timeless (Tim) antibody

The circadian rythms of behavior and hormones are controlled at the gene expression level. Both higher mammals and Drosophila essentially use homologues genes as molecular gears to control the circadian oscillations. In D. melanogaster the period and timeless genes are the 2 major player of its clock function and its auto-regulatory feed back loop for circadian rythms. In vertebrates the homologies of these genes are also known and are expressed in superchiasmatic nucleus in CNS. The mammalian homologues of fruit fly Timeless (TIMLS) gene is highly expressed in brain with weak circadian expression in superchiasmatic nucleus. In retina, the Timeless is expressed with robust circadian rhythm with light and dark cycle. In addition, TIMLS interacts with Per1, another member of the circadian-related gene in retina, the two together control the autoregulatory feedback mechanism of circadian oscillation (1).
In vertebrates, the homologues of D. melanogaster genes TIMLS and period (Per), are responsible for a variety of functions beside circadian rythms. Mammalian TIMLS exhibit 5 homologous regions with drosophila’s TIMLS gene and is critical to vertebrate embryonic development. The mouse TIMLS is expressed in all organs, the gene is significantly up-regulated in lung morphogenesis during early phases of development. In lung it is expressed in non-ciliated columnar epithelial cells. The conditional knockdown of TIMLS in superchiasmatic nucleus disrupts neuronal activity rythams and altered the known core clock elements (2). In concert with mouse period homolog the TIMLS functions as a negative feed back arm of the mammalian molecular clock (2). It is important to note that TIMLS levels do not oscillate in the SCN nor they are acutely altered by day light cycle. The yeast two hybrid assays revealed no interaction with period and TIMLS protein suggesting that in mouse circadian rythms, per-per interactions have replaced the per-TIMLS dimmers in the molecular functioning of mammalian clock (3).
TIMLS is a 1197 amino acid protein, was mapped on chromosome 12q12-13 in human and 10D3 in mouse. The C-terminal end of TIMLS protein also contained a SANT domain in this region. The mouse and human TIMLS share a 83% homology with fruit fly TILMS protein.

General readings

Sangoram,A.M., (2005) Mol. Cell. Biol. 25 (8), 3109-3116

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Timeless (Tim) antibody

Immunohistochemistry-Paraffin: Timeless Antibody [NB100-40853] - FFPE section of mouse teratoma.  Affinity purified rabbit anti-Timeless used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Timeless (Tim) antibody

WB Suggested Anti-TIMELESS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Timeless (Tim) antibody

WB Suggested Anti-TIMELESS Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Timeless (Tim) antibody

WB Suggested Anti-TIMELESS Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Sh, Ye WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Timeless (Tim) control peptide

Timeless (Tim)
0.1 mg / €250.00
  Acris Antibodies GmbH

Timeless (Tim) (C-term) antibody

Western blot of Timeless antibody (AP10336PU-N at 1/500 dilution). Rabbit IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Timeless (Tim) antibody

Timeless (Tim) Rabbit Serum Hu E, P, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Timeless (Tim) antibody

Timeless (Tim) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Timeless (Tim) antibody

Immunofluorescence of purified MaxPab antibody to TIMELESS on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Timeless (Tim) antibody

Timeless (Tim) Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Timeless (Tim) antibody

TIMELESS MaxPab rabbit polyclonal antibody. Western Blot analysis of TIMELESS expression in human colon. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Timeless (Tim) antibody

Human kidney; TIMELESS antibody - N-terminal region (ARP34186_P050) in Human kidney cells using Immunohistochemistry Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Timeless (Tim) antibody

Transfected 293T; WB Suggested Anti-TIMELESS Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; TIMELESS antibody - N-terminal region (ARP38403_T100) in Transfected 293T cells using Western Blot Rabbit IgG Purified African clawed frog, Bov, Can, Dros, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Timeless (Tim) antibody

Transfected 293T; WB Suggested Anti-TIMELESS Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; TIMELESS antibody - C-terminal region (ARP38404_T100) in Transfected 293T cells using Western Blot Rabbit IgG Purified Can, Dros, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Timeless (Tim) antibody

Immunofluorescence: Timeless Antibody [NBP1-32404]- analysis of HeLa, using TIMELESS antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Timeless (Tim) antibody

Western Blot: Timeless Antibody (EPR5274(2)) [NBP1-95967] - 12, and (F) NIH/3T3 cell lysates using anti-Timeless. Rabbit EPR5274(2) Purified Hu, Ms, Rt F, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Timeless (Tim)

Timeless (Tim) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Antibody Pairs

Catalog No.    

TIMELESS (Human) IP-WB Antibody Pair

TIMELESS (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

TIMELESS 293T Cell Transient Overexpression Lysate(Denatured)

TIMELESS 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

TIMELESS overexpression lysate

TIMELESS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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