TIMM50 antibody

Principal name

TIMM50 antibody

Alternative names for TIMM50 antibody

TIM50, PRO1512, Mitochondrial import inner membrane translocase subunit TIM50

SwissProt ID

Q3ZCQ8 (Human)

Gene ID

92609 (TIMM50)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of TIMM50 antibody

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.

General readings

Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES. Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. J Biol Chem. 2004 Jun 4;279(23):24813-25. Epub 2004 Mar 25.

2 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIMM50 antibody

  Goat Polyclonal Antibody against TIMM50  
TA302964 staining (0.5µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMM50 antibody

  Rabbit Polyclonal Anti-TIMM50 Antibody  
Host: Rabbit; Target Name: TIMM50; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

2 Item(s)

per page
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