TIMP3 antibody

Principal name

TIMP3 antibody

Alternative names for TIMP3 antibody

Protein MIG-5, TIMP-3, Metalloproteinase inhibitor 3, Tissue inhibitor of metalloproteinases 3

SwissProt ID

O73746 (Xenla), O97590 (Rabit), P26652 (Chick), P35625 (Human), P39876 (Mouse), P48032 (Rat), P79121 (Bovin), Q9TUL9 (Horse)

Gene ID

7078 (TIMP3), 373596 (timp3), 1027078 (yhcP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Bab (Baboon), Frog, Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TIMP3 antibody

The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightly bound inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot like structures. The N terminal region is necessary for inhibitory activity. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved to produce the mature protein. The C terminal regions are divergent and enhance the inhibition selectivity and binding efficiency. Although the TIMP proteins share high homology, following secretion they are localized extracellularly either in soluble form (TIMP1, TIMP2, and TIMP4) or bound to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinase 3 (TIMP3) was first purified from chicken embryo fibroblasts and identified as ChIMP3. The human homologue of TIMP3, was originally detected as an inducible serum protein in WI-38 fibroblasts. The TIMP3 localization differs from that of the other three TIMPs, and is thought to be primarily deposited into the extracellular matrix (ECM). TIMP3 is insoluble, binds to the ECM associated with a variety of cell types, and is widely distributed throughout the body. TIMP3 shows 30% amino acid homology with TIMP1 and 38% homology with TIMP2. TIMP3 has been shown to promote the detachment of transformed cells from ECM and to accelerate morphological changes associated with cell transformation. Furthermore, up regulation of TIMP 3 has been associated with a block in the G1 phase of the cell cycle during differentiation of HL60 leukemia cells. The human TIMP3 gene has the chromosomal location of 22q12-22q13. TIMP3 mRNA is highly expressed in placenta but is also found in the heart, kidney, lung, pancreas, uterus and skeletal muscle with low levels in the brain. In endometrium, TIMP3 is reported to be expressed in luminal epithelium, glands, stroma, endothelial cells and vascular smooth muscle cells. TIMP3 is also reported to be expressed by fibroblast like cells in ulcerated intestinal wall.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIMP3 (C-term) antibody

Human placenta stained with Anti-TIMP-3 antibody Rabbit Purified Hu, Rb P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

TIMP3 (C-term) antibody

Human placenta stained with Anti-TIMP-3 antibody Rabbit Purified Hu, Rb P, WB
1 ml / €440.00
  Acris Antibodies GmbH

TIMP3 (C-term) antibody

Human placenta stained with Anti-TIMP-3 antibody Rabbit Purified Hu, Rb P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

TIMP3 (175-211) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta stained with TIMP3 Antibody Cat.-No AP32837PU. Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

TIMP3 (175-211) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta stained with TIMP3 Antibody Cat.-No AP32837PU. Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

TIMP3 (175-211) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta stained with TIMP3 Antibody Cat.-No AP32837PU. Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rt F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

TIMP3 antibody

Western blot analysis of extracts from HUVEC cells, using TIMP3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP3 antibody

Predicted band size: 24 kDa . Positive control: Huvec cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP3 antibody

Predicted band size: 24 kDa . Positive control: Huvec cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP3 antibody

Western blot analysis on HuvEc cell lysate using TIMP3 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMP3 antibody

Western blot (WB) analysis of TIMP-3 antibody (Cat.-No. AP20507PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TIMP3 (C-term) antibody

Western blot analysis of rat kidney tissue lysis using TIMP3 antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TIMP3 (Center) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TIMP3 antibody

TIMP3 Mouse IgG2a 1D8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

TIMP3 antibody

TIMP3 Rabbit Frog, Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TIMP3 antibody

Western blot analysis of rat kidney tissue lysate. Using TIMP3 polyclonal antibody ( Cat # PAB8014 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TIMP3 antibody

Immunohistochemical staining of TIMP3 on formalin fixed, paraffin embedded human placenta with TIMP3 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

TIMP3 antibody

Western blot analysis of extracts from HuvEc cells, using TIMP3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

TIMP3 antibody

Western blot analysis of extracts from HuvEc cells, using TIMP3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

TIMP3 antibody

TIMP3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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