TIMP4 antibody

Principal name

TIMP4 antibody

Alternative names for TIMP4 antibody

TIMP-4, Tissue inhibitor of metalloproteinases 4, Metalloproteinase inhibitor 4

SwissProt ID

O97563 (Bovin), O97591 (Rabit), P81556 (Rat), Q99727 (Human), Q9JHB3 (Mouse)

Gene ID

7079 (TIMP4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of TIMP4 antibody

The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tight binding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIMP4 antibody

Western blot analysis of extracts from NIH/3T3 cells, using TIMP4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP4 antibody

Immunohistochemistry of Mouse Anti-TIMP4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: a) isotype specific IgG2bk monoclonal; b) TIMP-4 antibody (clone 9:4-7); and c) TIMP-4 each at 10 µg/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal. Mouse 9:4-7 Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMP4 antibody

Predicted band size: 26 kDa . Positive control: Human brain tissue, PC3 and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%+8%SDS-PAGE Lane1: Human brain tissue lysate Lane2: PC3 cell lysate Lane3: Hela cell lysate Lysates: 50 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP4 antibody

Predicted band size: 26 kDa . Positive control: Human brain tissue, PC3 and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%+8%SDS-PAGE Lane1: Human brain tissue lysate Lane2: PC3 cell lysate Lane3: Hela cell lysate Lysates: 50 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP4 antibody

Western blot analysis of TIMP4 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIMP4 antibody

Western blot analysis on NIH-3T3 cell lysate using TIMP4 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIMP4 antibody

TIMP4 Mouse IgG2a SB30c HRP Hu C, ICC/IF, WB
1 ml / €470.00
  Acris Antibodies GmbH

TIMP4 antibody

Immunocytochemistry / Immunofluorescence: Human pancreatic carcinoma cells fixed with methanol and stained with AM08201PU-N at 5 µg/ml. Mouse IgG2a SB30c Purified Hu C, ICC/IF, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

TIMP4 antibody

Immunohistochemistry (IHC) analyzes of TIMP4 antibody (Cat.-No AP06464PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TIMP4 (C-term) antibody

Western blot analysis of rat kidney tissue lysis using TIMP4 antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TIMP4 (Center) antibody

TIMP4 Antibody (Center) (AP54258PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TIMP4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TIMP4 antibody

Western Blot analysis of TIMP4 expression in transfected 293T cell line (H00007079-T01) by TIMP4 MaxPab polyclonal antibody.

Lane 1: TIMP4 transfected lysate(24.75 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TIMP4 antibody

TIMP4 monoclonal antibody, clone SB30c ( Cat # MAB3333 ) was stained on cold methanol fixed human pancraetic carcinoma cells with DAPI and Goat Anti-Mouse Ig, human adsorbed-FITC. Mouse IgG2a SB30c Hu C, ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TIMP4 antibody

Western blot analysis of rat kidney tissue lysate. Using TIMP4 polyclonal antibody ( Cat # PAB8015 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

TIMP4 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TIMP4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

TIMP4 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TIMP4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

TIMP4 antibody

TIMP4 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TIMP4 antibody

TIMP4 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

TIMP4 antibody

Immunohistochemical analysis of TIMP4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TIMP4 antibody

Immunohistochemical analysis of TIMP4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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