TIP47 / M6PRBP1 antibody

Principal name

TIP47 / M6PRBP1 antibody

Alternative names for TIP47 / M6PRBP1 antibody

Cargo selection protein TIP47, Placental protein 17, Mannose-6-phosphate receptor-binding protein 1, 47 kDa mannose 6-phosphate receptor-binding protein

SwissProt ID

O60664 (Human), Q5BLZ2 (Pig), Q9DBG5 (Mouse)

Gene ID

10226 (PLIN3), 66905 (Plin3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Por (Porcine), Prim (Primate)

Available hosts

Rabbit, Guinea Pig, Goat, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection))

Background of TIP47 / M6PRBP1 antibody

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin. It has been described as a placental protein, as well. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, this protein is probably significant as an oncodevelopmental marker.

General readings

1. Bulankina AV: TIP47 is recruited to lipid droplets and important for the organelle biogenesis and function. PhD Thesis University of Goettingen (2003)
2. Robenek H, Lorkowski S, Schnoor M, Troyer D. Spatial integration of TIP47 and adipophilin in macrophage lipid bodies. J Biol Chem. 2005 Feb 18;280(7):5789-94. Epub 2004 Nov 15. PubMed PMID: 15545278.
3. Robenek H, Robenek MJ, Buers I, Lorkowski S, Hofnagel O, Troyer D, et al. Lipid droplets gain PAT family proteins by interaction with specialized plasma membrane domains. J Biol Chem. 2005 Jul 15;280(28):26330-8. Epub 2005 May 16. PubMed PMID: 15897193.
4. Robenek H, Hofnagel O, Buers I, Lorkowski S, Schnoor M, Robenek MJ, et al. Butyrophilin controls milk fat globule secretion. Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10385-90. Epub 2006 Jun 26. PubMed PMID: 16801554.
5. Muthusamy K, Halbert G, Roberts F: Immunohistochemical staining for adipophilin, perilipin and TIP47 in ocular sebaceous gland carcinoma. J Clin Pathol (2006)
6. Straub BK, Stoeffel P, Heid H, Zimbelmann R, Schirmacher P: Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936-1946 (2008)
7. Bulankina AV, Deggerich A, Wenzel D, Mutenda K, Wittmann JG, Rudolph MG, Burger KNJ, Höning S: TIP47 functions in the biogenesis of lipid droplets. J Cell Biol 185, 641-655 (2009)
8. Straub BK, Herpel E, Singer S, Zimbelmann R, Breuhahn K, Macher-Goeppinger S, Warth A, Lehmann-Koch J, Longerich T, Heid H, Schirrmacher P: Lipid-droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Modern Pathology 23, 480-492 (2010)
9. Heid HW, Rickelt S, Zimbelmann R, Winter S, Schumacher H, Dörflinger Y (2013): Lipid droplets, perilipins and cytokeratins – unravelled liaisons in epithelial-derived cells (PLoS ONE 8(5): e63061. doi:10.1371/journal.pone.0063061)
10. Díaz E, Pfeffer SR. TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking. Cell. 1998 May 1;93(3):433-43. PubMed PMID: 9590177.
11 Than NG, Sumegi B, Than GN, Kispal G, Bohn H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. Tumour Biol. 1999 Jul-Aug;20(4):184-92. PubMed PMID: 10393528.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIP47 / M6PRBP1 (154-167) antibody


Staining of HeLa (lane 1) and Human Colon cancer (lane two) lysate using Perilipin 3 / TIP47 Antibody Cat.-No AP32072PU-N at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 antibody

Detection of TIP47 in 3T3 L1 lysate using anti-TIP47 Rabbit IgG Purified Hu, Por, Prim WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIP47 / M6PRBP1 antibody

TA303220 (0.1µg/ml) staining of Rat Skin lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIP47 / M6PRBP1 (C-term) antibody

Western blot analysis of TIP47 in Daudi cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIP47 / M6PRBP1 (N-term) antibody

Western blot analysis of TIP47 in EL4 cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIP47 / M6PRBP1 antibody

Western Blot: TIP47 Antibody -WB detection of TIP47 in 3T3 L1 lysate. Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TIP47 / M6PRBP1 (N-term) antibody

AP08870PU-N M6PRBP1 antibody staining of  Formalin-Fixed, Paraffin-Embedded (FFPE) Human Adrenal. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 (N-term) antibody

TIP47 / M6PRBP1 Guinea Pig Serum Ms, Rt C, P, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 antibody

IHC Image for AP22588PU-N
Mouse Spleen (formalin-fixed, paraffin-embedded) stained with M6PRBP1 antibody AP22588PU-N followed by biotinylated anti-goat IgG secondary antibody LS-D3, alkaline phosphatase-streptavidin and chromogen. Goat Purified Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 Control Peptide

TIP47 / M6PRBP1
50 µg / €160.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 Control Peptide

TIP47 / M6PRBP1
50 µg / €160.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 (C-term) antibody

TIP47 / M6PRBP1 Guinea Pig Serum Ms, Rt C, P, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 (N-term) antibody

TIP47 / M6PRBP1 Guinea Pig Serum Bov, Hu C, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 (C-term) antibody

TIP47 / M6PRBP1 Guinea Pig Serum Hu C, P, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

TIP47 / M6PRBP1 antibody

PLIN3 MaxPab polyclonal antibody. Western Blot analysis of PLIN3 expression in human pancreas. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TIP47 / M6PRBP1 antibody

PLIN3 MaxPab polyclonal antibody. Western Blot analysis of PLIN3 expression in human pancreas. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TIP47 / M6PRBP1 antibody

Immunoperoxidase of monoclonal antibody to M6PRBP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG1 2F7 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TIP47 / M6PRBP1 antibody

The M6prbp1 polyclonal antibody ( Cat # PAB7251 ) ( 0.1 µg/ml ) staining of rat skin lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TIP47 / M6PRBP1 antibody

Western blot analysis of Plin3 in 3T3-L1 lysate using Plin3 polyclonal antibody ( Cat # PAB12130 ). Rabbit Hu, Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

TIP47 / M6PRBP1 antibody

Western blot analysis of PLIN3 in 3T3-L1 lysate using PLIN3 polyclonal antibody ( Cat # PAB12391 ). Rabbit Hu, Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

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