TIPIN antibody

Principal name

TIPIN antibody

Alternative names for TIPIN antibody

TIMELESS-interacting protein

SwissProt ID

Q3ZCC4 (Bovin), Q4QR88 (Rat), Q5F416 (Chick), Q91WA1 (Mouse), Q9BVW5 (Human)

Gene ID

54962 (TIPIN)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), EPair for Elisa (EPair)

Background of TIPIN antibody

Timeless Interacting Protein (TIPIN) is a mammalian protein that interacts with Timeless (TMLS), which plays a role in DNA damage checkpoint responses. Mammalian TIM and its constitutive binding partner, TIPIN (ortholog of S.c. Csm3p, S.p. Swi3p), are replisome-associated proteins. Both proteins associate with components of the endogenous replication fork complex, and are present at BrdU-positive DNA replication sites. Further, the direct binding of the TIM-Tipin complex to the 34 kDa subunit of replication protein A provides a biochemical explanation for the potential coupling role of these proteins. Like TIM, TIPIN is also involved in the molecular mechanism of UV-dependent checkpoint activation and cell growth arrest. TIPIN additionally associates with peroxiredoxin2 and appears to be involved in checkpoint responses to H2O2, a role recently described for yeast versions of TIM and Tipin (1, 2).
TIPIN is a nuclear protein that associates with the replicative helicase and protects cells against genotoxic agents. Tipin is required for efficient cell cycle arrest in response to DNA damage, and a reduction in TIPIN makes cells sensitive to ionizing radiation as well as replication stress. Loss of TIPIN causes the cells to become spontaneous gamma-H2AX foci, a marker for DNA double-strand breaks. TIPIN and Timeless form a complex via interacting near the N-terminal motifs that maintains the level of both proteins in cells and that the loss of either one will lead to the loss of the interacting partner (2), suggesting that complex formation is required for stabilization and nuclear accumulation of both proteins. TIPIN is capable of regulating TMLS activity by disrupting the ability of TIPIN to form homo-multimeric complexes, TIMLS also forms a functional complex with TIPIN, and provide a starting point from which to link TIMLS to biochemical pathways controlling vital cellular functions. Mammalian TIPIN has 2 variants of 36kDa proteins, the mouse protein is slightly smaller (278aa) with significant homology to human TIPIN protein.

General readings

Dheekollu,J., et al. (2011) J. Virol. 85 (12), 5853-5863Kemp,M.G., et al. (2010) J. Biol. Chem. 285 (22), 16562-16571

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIPIN antibody

WB Suggested Anti-TIPIN Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysateTIPIN is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TIPIN antibody

WB Suggested Anti-TIPIN Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TIPIN antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPIN (RC201502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPIN. Mouse IgG2a OTI2E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TIPIN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802938) Mouse IgG1 OTI3A1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TIPIN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802939) Mouse IgG1 OTI2E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TIPIN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802940) Mouse IgG2b OTI2H6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TIPIN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802975) Mouse IgG1 OTI2C10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPIN (RC201502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPIN. Mouse IgG1 OTI3B11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TIPIN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802977) Mouse IgG1 OTI1H4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPIN (RC201502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPIN. Mouse IgG2b OTI1A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPIN (RC201502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPIN. Mouse IgG1 OTI1E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TIPIN control peptide

TIPIN
0.1 mg / €250.00
  Acris Antibodies GmbH

TIPIN (245-264) antibody

TIPIN Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TIPIN antibody

TIPIN Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

TIPIN antibody

Western Blot analysis of TIPIN expression in transfected 293T cell line (H00054962-T01) by TIPIN MaxPab polyclonal antibody.

Lane 1: TIPIN transfected lysate(33.11 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TIPIN antibody

TIPIN MaxPab rabbit polyclonal antibody. Western Blot analysis of TIPIN expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TIPIN antibody

Immunofluorescence of monoclonal antibody to TIPIN on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 1G11 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TIPIN antibody

Human Jurkat; WB Suggested Anti-TIPIN Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate. TIPIN is supported by BioGPS gene expression data to be expressed in Jurkat.; TIPIN antibody - N-terminal region (ARP50620_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

TIPIN antibody

Human Heart; WB Suggested Anti-TIPIN Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart; TIPIN antibody - middle region (ARP50621_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

TIPIN antibody

TIPIN Rabbit Serum Hu, Ms, Rt C, P, WB
0.2 ml / €400.00
  Novus Biologicals Inc.

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