TLK1 antibody

Principal name

TLK1 antibody

Alternative names for TLK1 antibody

Tousled-like kinase 1, KIAA0137, Serine/threonine-protein kinase tousled-like 1, PKU-beta

SwissProt ID

Q8C0V0 (Mouse), Q9UKI8 (Human)

Gene ID

9874 (TLK1)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Mky (Monkey), Can (Canine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of TLK1 antibody

Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage. Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent. Tlk-1 heterodimerizes with Tlk-2. Multiple isoforms of the protein have been detected. Mature Tlk-1 is reported to have an apparent molecular weight of ~86kDa, ~89kDa and ~64kDa corresponding to isoforms 1, 2 and 3 respectively.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TLK1 antibody

Western blot analysis of extracts from rat brain cells and NIH/3T3 cells, using TLK1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TLK1 pSer695 antibody

WB using Anti-Tlk-1 antibody shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). The primary antibody diluted to 1:250 for 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at room temperature. Rabbit Aff - Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

TLK1 antibody

WB Suggested Anti-TLK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: ACHN cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

TLK1 antibody

WB Suggested Anti-TLK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysateTLK1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

TLK1 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TLK1 antibody

TLK1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TLK1 antibody

Immunoperoxidase of monoclonal antibody to TLK1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml] Mouse IgG3 4B3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TLK1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with TLK1 polyclonal antibody ( Cat # PAB3318 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

TLK1 antibody

Western blot using TLK1 polyclonal antibody ( Cat # PAB10238 ) shows detection of aband ~ 64 kDa corresponding to isoform 3 of human TLK1 ( arrowhead ).
Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide ( data not shown ).
Approximately 30 µg of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 250 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature. Chimp, Chk, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

TLK1 antibody

Human 721_B; WB Suggested Anti-TLK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. TLK1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; TLK1 antibody - N-terminal region (ARP58340_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

TLK1 antibody

TLK1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TLK1 antibody

Western blot analysis of TLK1 expression in HeLa (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TLK1 antibody

Western blot analysis of TLK1 expression in HeLa (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TLK1 antibody

Western blot analysis of TLK1 expression in HeLa (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

TLK1 antibody

Western blot analysis of TLK1 expression in HeLa (A); A431 (B); NIH3T3 (C); rat thymus (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TLK1 antibody

Western blot analysis of TLK1 expression in HeLa (A); A431 (B); NIH3T3 (C); rat thymus (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TLK1 antibody

Western blot analysis of TLK1 expression in HeLa (A); A431 (B); NIH3T3 (C); rat thymus (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

TLK1 pSer743 antibody

Western blot analysis of TLK1 (pS743) expression in MCF7 (A); rat brain (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Rt WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

TLK1 pSer743 antibody

Western blot analysis of TLK1 (pS743) expression in MCF7 (A); rat brain (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Rt WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

TLK1 pSer743 antibody

Western blot analysis of TLK1 (pS743) expression in MCF7 (A); rat brain (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Rt WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

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