TLR5 antibody

Principal name

TLR5 antibody

Alternative names for TLR5 antibody

Toll-like receptor 5, TIL3, Toll/interleukin-1 receptor-like protein 3

SwissProt ID

O60602 (Human), Q9JLF7 (Mouse)

Gene ID

7100 (TLR5), 53791 (Tlr5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Fe (Feline), Por (Porcine)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Dot blot (Dot), Frozen Sections (C)

Background of TLR5 antibody

The mammalian host defense system is essentially regulated by highly conserved Toll-like receptor (TLR) family of proteins. At least 13 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens and activation of innate immunity. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM) and caused production of inflammatory cytokines such as IL-1, IL-6, IL-8 , TNFa, and IL-12, chemokines and co-stimulatory molecules such as CD40, CD80 and CD86. In the presence of inflammatory cytokines and binding of adaptor molecule, MyD88 that binds FADD and triggers apoptosis through the caspase cascade. TLR induced apoptosis pathway appears to be a repertoire of defense mechanism utilized by innate defense mechanism. The constitutive expression of many human TLRs (1, 2, 3) have been shown on the surface of myeloid lineage cells by RT-PCR and use of specific monoclonal antibodies. Upon activation of these receptors by their respective chemokines and ligands have been shown in literature on various cell lines including endothelial, epithelial and other cells. The expression of TLR 3, 7, 8 and 9 are mainly found on endosomal lysosomal compartments. Human TLR3 is expressed in human fibroblasts cells and TLR 9 in in-vitro derived DC cells. There is significant evidence of TLR involvement is many systemic disorders following bacterial infection including sepsis, peridontitis, cardiac ischemia, cerebral palsy and others, understanding the TLRs involvement in these conditions will allow therapeutic interventions at the receptor level for treatment of these disorders.
The TLR are highly conserved protein and share structural and functional domains across species. These receptors recognize pathogen associated molecular patters (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines needed for production of immediate immunity. TLR2 and TLR5 mRNA was detected in the CD8(+) T cells from cord blood. The expression of TLR4 and TLR5 are reduced in asthmatic patients which may be partly responsible for immunopathological mechanism of asthma by reducing the release of Th1 anti-inflammatory cytokines (4). The first human toll like receptor to be identified, TLR4, senses lipopolysaccharide (LPS) while TLR2 on the other hand senses diacylated or triacylated lipopeptides after heterodimerizing with either TLR6 or TLR1, respectively. The conserved cytoplasmic TIR (Toll/IL-1 receptor) domains of the IL-1 and Toll-like receptors are the critical focal point for the generation of ligand-induced cytoplasmic signaling cascades. For signaling all the TLRs utilize one or more of the four known TIR-containing adaptor molecules: MyD88, TIRAP/MAL, TRIF, and TRAM. Human TLR2 is a 839 amino acid (105 kDa) type I trans-membrane receptor protein characterized by extracellular domains with leucine-rich repeats and a cytoplasmic domain with type I IL-1 receptor. The TLR 2 and 4 are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and is involved in innate immunity. TLR4 is expressed on peripheral blood and together with MD-2 and CD14 is responsible for LPS signaling recognition. Many specific adapter molecules (TICAM, MyD88, ICAM2, TRAM, TIRAP, TRIF etc) are also involved in signaling of several other TLRs.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TLR5 antibody

Western Blot: TLR5 Antibody (19D759.2) [TA337067] - BEAS-2B cells whole cell lysate. Image from verified customer review. Mouse IgG2a 19D759.2 Purified Can, Hu, Ms C, F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TLR5 antibody

Immunohistochemical analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control ( top ) and TLR5 monoclonal antibody, clone 19D759.2 ( Cat # MAB0109 ) ( bottom ) at 5 µg/ml. Mouse IgG2a 19D759.2 Hu, Ms F, P, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

TLR5 (Center) antibody

Western blot analysis of TLR5 in rat brain cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TLR5 (C-term) antibody

Western blot analysis of TLR5 in THP-1 cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TLR5 antibody

Anti-TLR5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit IgG Purified Hu, Ms, Rt
50 µg / €345.00
  OriGene Technologies, Inc.

TLR5 antibody

Western Blot: TLR5 Antibody (85B152.5) [TA336873] - Normal human bronchial epithelial total cell lysate. Image from verified customer review. Mouse IgG2a 85B152.5 Purified Can, Hu, Ms F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TLR5 antibody

Western Blot: TLR5 Antibody [TA337069] - THP-1 cell lysate. Image from verified customer review. Rabbit IgG Purified Hu, Ms, Por, Rt F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TLR5 (C-term) antibody

Western blot analysis of TLR5 in THP-1 cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TLR5 antibody

Western blot analysis of TLR5 in rat brain cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TLR5 (C-term) antibody

Lung: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TLR5 antibody

Figure 2. Staining TLR5 in Lung, Alveolar Macrophages by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TLR5 Control Peptide

TLR5
0.1 mg / €250.00
  Acris Antibodies GmbH

TLR5 (200-219) antibody

TLR5 Rabbit IgG Aff - Purified Fe, Hu, Ms, Por Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TLR5 (N-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TLR5 (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TLR5 Control Peptide

TLR5
50 µg / €160.00
  Acris Antibodies GmbH

TLR5 Control Peptide

TLR5
50 µg / €160.00
  Acris Antibodies GmbH

TLR5 antibody

TLR5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TLR5 antibody

Detection limit for recombinant GST tagged TLR5 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2b 4F7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TLR5 antibody

Detection limit for recombinant GST tagged TLR5 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 3E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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