TNF-beta / Lymphotoxin-alpha antibody

Principal name

TNF-beta / Lymphotoxin-alpha antibody

Alternative names for TNF-beta / Lymphotoxin-alpha antibody

TNFB, TNFSF1, LTA, LT-alpha, Tumor necrosis factor ligand superfamily member 1

SwissProt ID

P01374 (Human), P09225 (Mouse), P10154 (Rabit), P26445 (Pig), Q06332 (Rat), Q06600 (Bovin), Q5WR07 (Canfa)

Gene ID

4049 (LTA), 16992 (Lta)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse, Sheep, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Neutralisation (NEUT), Frozen Sections (C)

Background of TNF-beta / Lymphotoxin-alpha antibody

Tumor Necrosis Factor (TNF) was originally discovered as a serum protein with necrotizing effects on certain transplantable mouse tumors in vivo and cytotoxic effects against some transformed cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin. Soluble TNF-beta is a T-cell derived glycoprotein of 25 kD mainly produced by activated lymphocytes. TNF-beta is 28 % structurally homologous with the macrophage produced non-glycoprotein TNF-alpha. TNF-beta is induced in an antigen-specific MHC restricted fashion from class I and class II restricted T cells. Viral infection is also associated with TNF-beta production by lymphoid cells. TNF-beta has several effects on target cells including killing, growth stimulation, induction of adhesion molecule (ICAM-1) expression, and induction of differentiation. The mechanisms of TNF-beta effects involve receptor binding and internalization and several sequelae including changes in prostaglandins and chromosome integrity. TNF-beta participates in tumor immunity, and it has been reported to inhibit carcinogenesis as well as growth of some tumors in vivo. The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF-beta without TNF-alpha cross reactivity.

General readings

General / background references:Granger GA & Kolb WP Lymphocyte in vitro cytotoxicity: mechanisms of immune and non-immune small lymphocyte mediated target L cell destruction. J Immunol 101:111-20 (1968). PubMed PMID: 5690881
Williams TW & Granger GA Lymphocyte in vitro cytotoxicity: lymphotoxins of several mammalian species. Nature 219:1076-7 (1968). PubMed PMID: 5673378
Ruddle NH & Waksman BH Cytotoxic effect of lymphocyte-antigen interaction in delayed hypersensitivity. Science 157:1060-2 (1967). PubMed PMID: 6036235

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TNF-beta / Lymphotoxin-alpha antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using LTA antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TNF-beta / Lymphotoxin-alpha antibody

IHC of human breast malignant carcinoma. The recommended concentration is 0.50 ug/mL with an overnight incubation at 4.C. An HRP-labeled polymer detection system was  used with a DAB chromogen. Heat induced  antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may  vary.    Tissue samples were provided  by the Cooperative Human Tissue Network, which is funded by the National Cancer  Institute. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TNF-beta / Lymphotoxin-alpha antibody

Immunofluorescence of human HeLa cells stained with monoclonal anti-human LTA antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue). Mouse IgG2b AT15A3 Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

Immunofluorescence of human HeLa cells stained with monoclonal anti-human LTA antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue). Mouse IgG2b AT15A3 Purified Hu, Ms E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 #1 Azide Free NEUT
0.1 mg / €360.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

Western blot (WB) analysis of LT-? antibody (Cat.-No.: AP06717PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha (Center) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

Western blot analysis of TNF-? antibody (Cat.-No.: AP20614PU-N) in extracts from COS-7cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Rabbit Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Rabbit Aff - Purified Hu E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TNF-beta / Lymphotoxin-alpha antibody

Western Blot analysis of LTA expression in transfected 293T cell line (H00004049-T01) by LTA MaxPab polyclonal antibody.

Lane 1: LTA transfected lysate(22.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 2B6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 4C7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 4C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 1G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 4G12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 3E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TNF-beta / Lymphotoxin-alpha antibody

TNF-beta / Lymphotoxin-alpha Mouse IgG1 1B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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