TNFAIP3 antibody

Principal name

TNFAIP3 antibody

Alternative names for TNFAIP3 antibody

OTUD7C, Zinc finger protein A20, Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20

SwissProt ID

P21580 (Human), Q60769 (Mouse)

Gene ID

7128 (TNFAIP3)

Available reactivities

Hu (Human), Ms (Mouse), Bt (Bat), Bov (Bovine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of TNFAIP3 antibody

A20, also referred to as TNF-a-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-?B. It was first identified as a TNF-a inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs. Constitutive expression of A20 is observed in lymphoid tissues, but it is transiently expressed in a variety of cell types in response to inflammatory sigls such as TNF-a, IL-1, phorbol esters, and LPS . Expression of A20 can confer resistance to apoptosis and NF-?B activation triggered by these sigls, probably through interference with TRAF (TNF receptor associated factor) family members , and interaction with the NF-?B inhibiting protein ABIN. Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions. The amino-terminus of A20 contains de-ubiquititing (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TNFAIP3 (C-term) antibody

Western blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 antibody at 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TNFAIP3 (Center) antibody

Western blot analysis of TNFAIP3 in rat lung tissue lysate with TNFAIP3 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TNFAIP3 antibody

Western blot analysis of extracts from RAW264.7 cells, using TNAP3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TNFAIP3 antibody

Western blot analysis of extracts of various mouse tissues, using TNFAIP3 antibody Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TNFAIP3 antibody

Western Blot: TNFAIP3 Antibody (59A426) [TA336834] - analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 cleavage band* not seen in the untreated control © THP1 cells. A20 Mouse IgG1 59A426 Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TNFAIP3 antibody

Host: Rabbit; Target Name: TNFAIP3; Sample Tissue: ACHN whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

TNFAIP3 antibody

Small intestine, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Bt, Hst, Hu, Mky, Ms, Por, Prim, Rb P
50 µg / €470.00
  Acris Antibodies GmbH

TNFAIP3 (C-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TNFAIP3 antibody

TNFAIP3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TNFAIP3 antibody

Detection of TNFAIP3 by Western blot in HT1080 cells, wild type. Or transfected with TNFAIP3 were activated with 0.1 µg/mL of rhs TNF-a for 4 hours. TNFAIP3 monoclonal antibody, clone 8E8.38 ( Cat # MAB3716 ) at 1 : 1,000 dilution. Mouse IgG1 8E8.38 Hu WB
50 µg / €730.00
  Abnova Taiwan Corp.

TNFAIP3 antibody

Immunofluorescence analysis of HepG2 cells, using TNAP3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

TNFAIP3 antibody

Immunofluorescence analysis of HepG2 cells, using TNAP3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

TNFAIP3 antibody

Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TNFAIP3 antibody

Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TNFAIP3 antibody

Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TNFAIP3

TNFAIP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

TNFAIP3

TNFAIP3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

TNFAIP3

TNFAIP3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

TNFAIP3

TNFAIP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TNFAIP3

TNFAIP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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