TNK2 / ACK1 antibody

Principal name

TNK2 / ACK1 antibody

Alternative names for TNK2 / ACK1 antibody

Activated CDC42 kinase 1

SwissProt ID

O54967 (Mouse), Q07912 (Human), Q17R13 (Bovin), Q5U2X5 (Rat)

Gene ID

10188 (TNK2), 51789 (Tnk2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Goat, Mouse, Rabbit, Chicken

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of TNK2 / ACK1 antibody

ACK1 (activated Cdc42-associated kinase 1) is a nonreceptor tyrosine kinase and the only tyrosine kinase known to interact with Cdc42. ACK1 phosphorylates and activates the guanine nucleotide exchange factor Dbl, which in turn directs the Rho family GTP-binding proteins (1). ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) via its C-terminal proline-rich domain. The ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) by its C-terminal praline rich domain. ACK1 also acts as a regulator of Dbl, which in turn activates Rho family proteins. There are at least 2 ACK1 associated proteins (Cdc42 and Grb) that are required for ACK1-dependent EGF signaling in the cell. The central motif in ACK1 has a clatherin adaptor that co-localizes ACK1 with in the clatherin containing vesicles that are involved in the trafficking of receptor mediated endocytosis (2).
The ACK family of non-receptor tyrosine kinase (ACK1 and ACK2) specifically associate with Cdc42 to modulate its activity in several signaling pathways. ACK1 acts as a mediator of EGF signal to Rho-family GTP-binding proteins (3). The activation of ACK1 involves auto-phoshorylation at Tyrosine 284, upon phosphorylation, the ACK1 activity is significantly enhanced. Tyrsine substitution at 284 by Phenylalnine (Y284F) and expression in COS-7 cells exhibit dramatically reduced levels of tyrosine phosphorylation (1). ACK2 mediates cell adhesion signals initiated by integrins b1 in a Cdc-dependent manner (4). There are three structural domins assocated with ACK1 protein, a N-terminal tyrosine kinase domain, SH3 domain, followed by a Cdc42/Rac interactive binding (CRIB) domain and a praline rich region near C-terminal end. ACK2 has been shown to be activated by Cdc42 binding to the CRIB region (4).
The ACK1 protein is a approximately 118-120kDa (1036 amino acids) protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TNK2 / ACK1 antibody

Western blot analysis of extracts of various cell lines, using TNK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TNK2 / ACK1 antibody

Western blot analysis of extracts from A549 cells, using ACK1 (Ab-284) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TNK2 / ACK1 antibody

Predicted band size: 119 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/1000-5000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TNK2 / ACK1 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using TNK2 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TNK2 / ACK1 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus and gastric cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using TNK2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TNK2 / ACK1 Control Peptide

TNK2 / ACK1
0.25 mg / €250.00
  Acris Antibodies GmbH

TNK2 / ACK1 antibody

IP with ACK1 antibody (AP09880PU-N at 1/250) using 250 µl of solubilized COS1 cells expressing active form of ACK1. Rabbit Aff - Purified Hu, Mky, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TNK2 / ACK1 control peptide

TNK2 / ACK1
0.1 mg / €270.00
  Acris Antibodies GmbH

TNK2 / ACK1 (phosphospecific) antibody

TNK2 / ACK1 Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

TNK2 / ACK1 pTyr518 antibody

Dot blot analysis of anti-Phospho-ACK1 pTyr518 Antibody (Cat.#AP12997PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

TNK2 / ACK1 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TNK2 / ACK1 (Center) antibody

Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (Center) (AP17079PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein). Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TNK2 / ACK1 (N-term) antibody

Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term) (AP17080PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein). Rabbit Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TNK2 / ACK1 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TNK2 / ACK1 pTyr284 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody AP55705PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

TNK2 / ACK1 pTyr284 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody AP55705PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €270.00
  Acris Antibodies GmbH

TNK2 / ACK1 antibody

TNK2 polyclonal antibody (A01), Lot # 051109JC01 Western Blot analysis of TNK2 expression in Y-79 ( Cat # L042V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TNK2 / ACK1 antibody

TNK2 / ACK1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TNK2 / ACK1 antibody

Immunoprecipitation of TNK2 transfected lysate using anti-TNK2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TNK2 MaxPab mouse polyclonal antibody (B01) (H00010188-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

TNK2 / ACK1 antibody

Western Blot analysis of TNK2 expression in transfected 293T cell line (H00010188-T02) by TNK2 MaxPab polyclonal antibody.

Lane 1: TNK2 transfected lysate(39.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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