TNK2 / ACK1 antibody

Principal name

TNK2 / ACK1 antibody

Alternative names for TNK2 / ACK1 antibody

Activated CDC42 kinase 1

SwissProt ID

O54967 (Mouse), Q07912 (Human), Q17R13 (Bovin), Q5U2X5 (Rat)

Gene ID

10188 (TNK2), 51789 (Tnk2), 303882

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TNK2 / ACK1 antibody

ACK1 (activated Cdc42-associated kinase 1) is a nonreceptor tyrosine kinase and the only tyrosine kinase known to interact with Cdc42. ACK1 phosphorylates and activates the guanine nucleotide exchange factor Dbl, which in turn directs the Rho family GTP-binding proteins (1). ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) via its C-terminal proline-rich domain. The ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) by its C-terminal praline rich domain. ACK1 also acts as a regulator of Dbl, which in turn activates Rho family proteins. There are at least 2 ACK1 associated proteins (Cdc42 and Grb) that are required for ACK1-dependent EGF signaling in the cell. The central motif in ACK1 has a clatherin adaptor that co-localizes ACK1 with in the clatherin containing vesicles that are involved in the trafficking of receptor mediated endocytosis (2).
The ACK family of non-receptor tyrosine kinase (ACK1 and ACK2) specifically associate with Cdc42 to modulate its activity in several signaling pathways. ACK1 acts as a mediator of EGF signal to Rho-family GTP-binding proteins (3). The activation of ACK1 involves auto-phoshorylation at Tyrosine 284, upon phosphorylation, the ACK1 activity is significantly enhanced. Tyrsine substitution at 284 by Phenylalnine (Y284F) and expression in COS-7 cells exhibit dramatically reduced levels of tyrosine phosphorylation (1). ACK2 mediates cell adhesion signals initiated by integrins b1 in a Cdc-dependent manner (4). There are three structural domins assocated with ACK1 protein, a N-terminal tyrosine kinase domain, SH3 domain, followed by a Cdc42/Rac interactive binding (CRIB) domain and a praline rich region near C-terminal end. ACK2 has been shown to be activated by Cdc42 binding to the CRIB region (4).
The ACK1 protein is a approximately 118-120kDa (1036 amino acids) protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TNK2 / ACK1 (N-term) antibody

Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term) (AP17080PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein). Rabbit Hu P, WB
0.4 ml / €370.00
  OriGene Technologies GmbH

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