TOB1 antibody

Principal name

TOB1 antibody

Alternative names for TOB1 antibody

TOB, TROB1, Transducer of erbB-2 1

SwissProt ID

P50616 (Human), Q61471 (Mouse), Q8R5K6 (Rat)

Gene ID

22057, 10140 (TOB1), 1014012 (apt)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C)

Background of TOB1 antibody

The Tob/Btg family of proteins consists of a large number of members among them Pc3/Tis21/Btg2, Btg1, Ana/Btg3, Pc3k, Tob2 and Tob. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types (Tirone). The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob (Matsuda et al). However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins (Yoshida et al).

General readings

Zhong,Z., et al., (2004) Oncogene 23 (39), 6630-6638

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TOB1 antibody

Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using TOB1 (Ab-164) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TOB1 antibody

WB Suggested Anti-TOB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

TOB1 antibody

WB Suggested Anti-TOB1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TOB1 antibody

TOB1 Rabbit IgG Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

TOB1 (N-term) antibody

Formalin-Fixed, Paraffin-Eembedded Human hepatocarcinoma reacted with TOB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TOB1 pSer164 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody AP55833PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

TOB1 pSer164 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody AP55833PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €270.00
  Acris Antibodies GmbH

TOB1 antibody

Immunoperoxidase of purified MaxPab antibody to TOB1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

TOB1 antibody

TOB1 Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

TOB1 pSer164 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using TOB1 (Phospho-Ser164) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

TOB1 pSer164 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using TOB1 (Phospho-Ser164) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

TOB1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TOB1 (Ab-164) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

TOB1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TOB1 (Ab-164) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

TOB1 antibody

Mouse NIH-3T3; WB Suggested Anti-TOB1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: NIH/3T3 cell lysate; TOB1 antibody - middle region (ARP36996_T100) in Mouse NIH-3T3 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

TOB1 antibody

TOB1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TOB1 antibody

TOB1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

TOB1 antibody

TOB1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

TOB1 antibody

TOB1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TOB1

TOB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

TOB1

TOB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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