TOLLIP antibody

Principal name

TOLLIP antibody

Alternative names for TOLLIP antibody

Toll-interacting protein

SwissProt ID

A2RUW1 (Rat), Q9H0E2 (Human), Q9QZ06 (Mouse)

Gene ID

54472 (TOLLIP), 54473 (Tollip), 6454472 (accD)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), EPair for Elisa (EPair)

Background of TOLLIP antibody

Members of the Toll-like receptor (TLR) family, med for the closely related Toll receptor in Drosophila, play a pivotal role in inte immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-?B and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of I?B, which normally maintains NF-?B in an ictive state by sequestering it in the cytoplasm. Tollip (Toll interacting protein) is an adaptor protein discovered to be associated with the IRAK complex and recruited to IL1-R following IL-1 stimulation. Overexpression of Tollip results in impaired NF-?B sigling. Tollip also associates directly with TLR2 and TLR4 and inhibits TLR-mediated sigling through inhibition of IRAK. Studies of Tollip deficient mice suggest that it plays a role in the regulation of inflammatory cytokines in response to IL-1 and LPS.

General readings

1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
2. Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
3. McGettrick AF and O and O'Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor
signal transduction. Mol. Imm. 2004; 41:577-82.
4. Zhang G and Ghosh S. Negative regulation of Toll-like receptor-mediated signaling by Tollip. J. Biol. Chem. 2002; 277:7059-65.
5. UniProtKB/Swiss-Prot entry Q9H0E2. http://cn.expasy.org/uniprot/Q9H0E2, Accessed on February 28, 2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TOLLIP (C-term) antibody

Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TOLLIP (Center) antibody

Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 0.5, (B) 1 and (C) 2ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TOLLIP antibody

Western blot analysis of extracts from HuvEc/Jurkat/293 cells, using TOLLIP antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TOLLIP antibody

Western blot analysis of extracts of PC-3 cell lines, using TOLLIP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

TOLLIP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOLLIP (RC200227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOLLIP. Mouse IgG1 OTI1D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TOLLIP antibody

Anti-TOLLIP mouse monoclonal antibody (TA507015) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TOLLIP(RC200227). Mouse IgG1 OTI2E6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TOLLIP antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TOLLIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507039) Mouse IgG1 OTI2A4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TOLLIP antibody

Western Blot: Mouse anti-Human Tollip antibody AM08202PU-N at 2 µg on pancreatic cancer cell lysate with Goat Anti-Mouse IgG2a, human adsorbed to HRP at 1/4000 dilution. Mouse IgG2a SB40a Purified Hu E, F, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

TOLLIP antibody

Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tollip antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : HepG2 cell lysate Lane 3 : HeLa cell lysate. Mouse IgG2a k1B17 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

TOLLIP antibody

Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tollip antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : HepG2 cell lysate Lane 3 : HeLa cell lysate. Mouse IgG2a k1B17 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

TOLLIP (C-term) antibody

Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TOLLIP antibody

Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

TOLLIP antibody

Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TOLLIP antibody

Figure 1. Staining TOLLIP in Brain, Cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TOLLIP Control Peptide

TOLLIP
50 µg / €160.00
  Acris Antibodies GmbH

TOLLIP Control Peptide

TOLLIP
50 µg / €160.00
  Acris Antibodies GmbH

TOLLIP antibody

TOLLIP MaxPab polyclonal antibody. Western Blot analysis of TOLLIP expression in rat brain. Mouse Serum Hu, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TOLLIP antibody

TOLLIP MaxPab polyclonal antibody. Western Blot analysis of TOLLIP expression in rat brain. Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

TOLLIP antibody

TOLLIP MaxPab rabbit polyclonal antibody. Western Blot analysis of TOLLIP expression in HepG2. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

TOLLIP antibody

TOLLIP MaxPab rabbit polyclonal antibody. Western Blot analysis of TOLLIP expression in mouse brain. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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