TOMM22 antibody

Principal name

TOMM22 antibody

Alternative names for TOMM22 antibody

TOM22, Mitochondrial import receptor subunit TOM22 homolog, Translocase of outer membrane 22 kDa subunit homolog

SwissProt ID

Q9CPQ3 (Mouse), Q9NS69 (Human)

Gene ID

56993 (TOMM22)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of TOMM22 antibody

Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100 kDa core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.

General readings

General / background references:Saeki K et al. Identification of mammalian TOM22 as a subunit of the preprotein translocase of the mitochondrial outer membrane. J Biol Chem 275:31996-2002 (2000). PubMed PMID: 10900208
Suzuki H et al. Characterization of rat TOM40, a central component of the preprotein translocase of the mitochondrial outer membrane. J Biol Chem 275:37930-6 (2000). PubMed PMID: 10980201
Yano M et al. Identification and functional analysis of human Tom22 for protein import into mitochondria. Mol Cell Biol 20:7205-13 (2000). PubMed PMID: 10982837

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TOMM22 antibody

TOMM22 polyclonal antibody (A01), Lot # 051206JC01. Western Blot analysis of TOMM22 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TOMM22 antibody

Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2a 4G4 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOMM22 antibody

Anti-TOMM22 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG2a 4G4 Purified Hu, Ms, Rt WB
50 µg / €345.00
  OriGene Technologies, Inc.

TOMM22 antibody

TOMM22 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TOMM22 antibody

TOMM22 Mouse IgG1 1C9-2 Purified Hu, Mky ICC/IF, WB
50 µl / €340.00
  Novus Biologicals Inc.

TOMM22 antibody

Immunohistochemistry-Paraffin: TOMM22 Antibody [NBP1-80671] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TOMM22

TOMM22 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TOMM22

TOMM22 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

TOMM22

TOMM22 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TOMM22

TOMM22 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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