TOP1 / SCL-70 antibody

Principal name

TOP1 / SCL-70 antibody

Alternative names for TOP1 / SCL-70 antibody

Top-1, DNA topoisomerase 1, DNA topoisomerase I

SwissProt ID

P11387 (Human), P41512 (Xenla), Q04750 (Mouse), Q9WUL0 (Rat)

Gene ID

7150 (TOP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Human, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunodiffusion (ID), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TOP1 / SCL-70 antibody

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single-stranded nick which is then re-ligated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. In normal eukaryotic cells, DNA topoisomerase I does not show relevant fluctuations across the cell cycle, unlike DNA topoisomerase II alpha. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti-tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by 32P transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability. Renal tumors were found not to show any differential levels compared with normal kidney. Topo I may be useful for the evaluation of DNA topoisomerase I expression in normal tissues, solid tumors and in further studies of ovarian, colorectal, cervical and prostatic tumors.

General readings

General / background references:Champoux JJ DNA topoisomerases: structure, function, and mechanism. Annu Rev Biochem 70:369-413 (2001). PubMed PMID: 11395412
Takahashi H et al. Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis. Hybridoma 19:331-4 (2000). PubMed PMID: 11001406
Froelich-Ammon SJ & Osheroff N Topoisomerase poisons: harnessing the dark side of enzyme mechanism. J Biol Chem 270:21429-32 (1995). PubMed PMID: 7665550

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TOP1 / SCL-70 antibody

Human breast carcinoma stained with anti-topoisomerase I antibody Rabbit IgG Aff - Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

TOP1 / SCL-70 antibody

Human breast carcinoma stained with anti-topoisomerase I antibody Rabbit IgG Aff - Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

TOP1 / SCL-70 antibody

Human breast carcinoma stained with anti-topoisomerase I antibody Rabbit IgG Aff - Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

TOP1 / SCL-70 antibody

Western blot analysis of extracts from Jurkat/293 cells, using TOP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TOP1 / SCL-70 antibody

Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term) (Cat. #TA328117). TA328117 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Mouse IgG1 1291CT875.142.166 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

TOP1 / SCL-70 antibody

Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. 
Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 µg/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: HeLa. 
Lane 2: HepG2. 
Lane 3: HEK-293. 
Lane 4: SH-SY5Y. 
Lane 5: MDCK. 
Lane 6: PC12. 
Lane 7: CMT-93. 
Lane 8: Neuro 2A. 
Lane 9: 3T3. Mouse IgG1 23B11 Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

TOP1 / SCL-70 antibody

TOP1 / SCL-70 Human Purified Hu E, ID, WB
2 ml / €300.00
  Acris Antibodies GmbH

TOP1 / SCL-70 antibody

TOP1 / SCL-70 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

TOP1 / SCL-70 antibody

Immunoperoxidase of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. [antibody concentration 3 ug/ml] Mouse IgG1 1A1 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOP1 / SCL-70 (N-term) antibody

Human Placenta; WB Suggested Anti-TOP1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Placenta; TOP1 antibody - N-terminal region (ARP61035_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

TOP1 / SCL-70 antibody

TOP1 / SCL-70 Mouse IgG1 Mab1 Purified Hu WB
50 µl / €350.00
  Novus Biologicals Inc.

TOP1 / SCL-70 antibody

Western Blot and Immunoprecipitation: Topoisomerase I Antibody [NBP1-30481]-Whole cell lysate from HeLa and 293T cells. TOP1 was  less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482. Rabbit Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

TOP1 / SCL-70 antibody

Western Blot: Topoisomerase I Antibody [NBP1-30482]- Whole cell lysate from HeLa (5, 15 and 50 mcg), 293T  (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies:  Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for  WB at 0.04 mcg/ml. Rabbit Hu, Ms WB
0.1 ml / €440.00
  Novus Biologicals Inc.

TOP1 / SCL-70 antibody

Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TOP1 / SCL-70

TOP1 / SCL-70 Rabbit Aff - Purified Purified using immobilized mouse monoclonal anti Scl-70. Homogeneity is verified by SDS-PAGE. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with ELISA assays.
1 kU / €1,060.00
  Acris Antibodies GmbH

15 Item(s)

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