TOP2A antibody
Principal name
TOP2A antibody
Alternative names for TOP2A antibody
TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme,
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)
Background of TOP2A antibody
DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIalpha and Topo IIbeta. The 180 kDa isoform, Topo IIbeta, and the 170 kDa isoform, Topo IIalpha, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.
General readings
Yoshida,K., et al., (2006) Mol. Cell. Biol. 26 (9), 3414-3431
Immunoperoxidase of monoclonal antibody to TOP2A on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to TOP2A on HeLa cell. [antibody concentration 10 ug/ml]
TOP2A monoclonal antibody (M01), clone 1E2 Western Blot analysis of TOP2A expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged TOP2A is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-TOP2A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. TOP2A is supported by BioGPS gene expression data to be expressed in HepG2.; TOP2A antibody - C-terminal region (AVARP04007_T100) in Human HepG2 cells using Western Blot
Western blot analysis of HepG2 cell lysate using Topoisomerase II alpha antibody (GTX77787)
Immunohistochemistry: Topo II Alpha Antibody [NB110-40636] - Detection of Human Topo II Alpha by Immunohistochemistry. Sample: FFPE section of human larynx squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II Alpha (NB110-40636) used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
Immunohistochemistry: Topoisomerase II alpha (Topo IIa) Antibody (EP1102Y) [NB110-57623] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Topo II alpha(Cat # 110-57623).
Immunohistochemistry-Frozen: Topo II Alpha Antibody (EP1102Y) NB110-57623 (1:50) staining murine cortex sections.
Western Blot: Topoisomerase II alpha (Topo IIa) Antibody (EP1102Y) [NB110-57623] - Western blot analysis using anti- Topo IIa Rabbit Monoclonal on HeLa. (NB110-57623), dilution 1:10,000.
Immunohistochemistry (IHC) analyzes of Topo IIalpha antibody (Cat.-No.: AP01227PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Topo IIalpha antibody (Cat.-No.: AP01227PU-N) in extracts from LOVO cells.
Immunohistochemistry (IHC) analyzes of Topo IIα antibody (Cat.-No.: AP01370PU-N) in paraffin-embedded human liver carcinoma tissue
Western blot (WB) analysis of Topo IIα antibody (Cat.-No.: AP01370PU-N) in extracts from HT-29.
Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using Topoisomerase II alpha (GTX100689) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE GTX100689 diluted at 1:3000
Immunoprecipitation ( Native & denatured ) with TOP2A polyclonal antibody ( Cat # PAB13698 ). Use Protein A. Use 10 µg/mg Raji cell lysate.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to TOP2A on HeLa cell (antibody concentration 10 µg/ml).
TOP2A monoclonal antibody clone 1E2 Western Blot analysis of TOP2A expression in HeLa NE.
Detection limit for recombinant GST tagged TOP2A is approximately 0.3 ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (AP54331PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of TOP2A Antibody (C-term) (AP54331PU-N) in A375 cell line lysates (35 µg/lane). TOP2A (arrow) was detected using the purified Pab.
TOP2A/Topo? Antibody (C-term) (AP54332PU-N) western blot analysis in K562 cell line lysates (35 µg/lane). This demonstrates the TOP2A/Topo? antibody detected the TOP2A/Topo? protein (arrow).
Formalin-Fixed, Paraffin-Embedded Human lymph node stained with TOP2A antibody Cat.-No. AM10144SU-N using peroxidase conjugate and AEC chromogen. Note nuclear staining of germinal center B-cells.
Immunohistochemistry-Paraffin: Topo II Alpha Antibody [NBP1-86927] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunofluorescence: Topo II Alpha Antibody [NBP1-86927] Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
Immunohistochemistry-Paraffin: Topo II Alpha Antibody [NBP1-86928] - Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells
Immunofluorescence: Topo II Alpha Antibody [NBP1-86928] - 431 shows positivity in nucleus.
Western Blot: Topo II Alpha Antibody [NBP1-86928] Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemical detection of Topo IIα on paraffin embedded section of a human stomach cancer with AM26449SU-N.
Western blot analysis of Topo IIα expression in MRC5 at various times following serum stimulation of arrested cells using AM26449SU-N and anti-Cyclin D1.
Western blot analysis of Topo IIα expression in Jurkat (1), HL-60 (2) and IC2Tr (3) using AM26449SU-N.
Western blot analysis of phospho-DNA expression in Jurkat cells using AM26582AF-N
Immunohistochemical detection on DNA Topoisomerase II alpha on paraffin embedded section of human stomach with AM26595AF-N
Immunocytochemical detection of DNA Topoisomerase II alpha on acetone fixed HEp-II with AM26595AF-N.
Western blot analysis of DNA Topoisomerase II alpha expression in Jurkat (1), Raji (2) and WR10L (3) using AM26595AF-N.
Flow cytometric analysis of DNA Topoisomerase II alpha expression in Jurkat. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26595AF-N to the cells.
Western blot analysis of Topo IIα, β expression in Jurkat (1), (3) and Raji (2), (4) using AM26690AF-N ((1),(2); 5 μg/ml and (3),(4); 10 μg/ml).
Immunohistochemistry of paraffin-embedded human tonsillitis slide using 24641-1-AP( TOP2A Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis slide using 24641-1-AP( TOP2A Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using 24641-1-AP( TOP2A Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis slide using 24641-1-AP( TOP2A Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human tonsillitis slide using 24641-1-AP( TOP2A Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using 24641-1-AP( TOP2A Antibody) at dilution of 1:50
Immunofluorescent analysis of A431 cells using 24641-1-AP( TOP2A Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP( TOP2A Antibody) at dilution of 1:1000
HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP( TOP2A Antibody) at dilution of 1:3000
Mouse testis tissue were subjected to SDS PAGE followed by western blot with 24641-1-AP( TOP2A Antibody) at dilution of 1:3000
IP Result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.
Western blot analysis of extracts from HepG2 cells treated with Ca2+ using TOP2A (Phospho-Thr1343) Antibody AP55782PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (TA802514) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802517)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
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- Highly Validated (17)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA802384 | TOP2A antibody |
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Mouse | IgG2b | OTI2A9 | Purified | Hu | P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +13 additional images |
Top Selling product! Highly validated antibody!
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| UM800042 | TOP2A antibody |
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Mouse | IgG1 | UMAB145 | Purified | Hu | P |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
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| UM800043 | TOP2A antibody |
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Mouse | IgG1 | UMAB146 | Purified | Hu | P, WB |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
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| UM870042 | TOP2A antibody |
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Mouse | IgG1 | UMAB145 | Purified | Hu | P |
30 µl /
€140.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
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| UM870043 | TOP2A antibody |
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Mouse | IgG1 | UMAB146 | Purified | Hu | P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA802385 | TOP2A antibody |
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Mouse | IgG1 | OTI2F8 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
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| TA802450 | TOP2A antibody |
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Mouse | IgG1 | OTI1D5 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA802499 | TOP2A antibody |
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Mouse | IgG1 | OTIE1 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
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| TA802505 | TOP2A antibody |
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Mouse | IgG1 | OTIB4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA802515 | TOP2A antibody |
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Mouse | IgG1 | OTI5E6 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| TA802516 | TOP2A antibody |
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Mouse | IgG1 | OTI2D12 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| TA802536 | TOP2A antibody |
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Mouse | IgG1 | OTI19D4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA802537 | TOP2A antibody |
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 |
Mouse | IgG1 | OTIE11 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA802684 | TOP2A antibody |
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 |
Mouse | IgG1 | OTI1D2 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| AM20953PU-N | TOP2A antibody |
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Mouse | IgG1 | 1E2 | Hu | E, ICC/IF, P, WB |
50 µg /
€530.00
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| Acris Antibodies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
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| AM26595AF-N | TOP2A (C-term) antibody |
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Mouse | IgG2a | 1C5 | Azide Free | Hu | F, ICC/IF, IP, P, WB |
0.1 mg /
€380.00
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| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| H00007153-M01 | TOP2A antibody |
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![Immunoperoxidase of monoclonal antibody to TOP2A on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00007153-M01-3-12-1-L.jpg) |
Mouse | IgG1 | 1E2 | Purified | Hu | E, ICC/IF, P, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA313198 | TOP2A antibody |
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 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA313199 | TOP2A antibody |
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA313200 | TOP2A antibody |
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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