TOP2A antibody
Principal name
TOP2A antibody
Alternative names for TOP2A antibody
TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme,
Gene ID
7153 (TOP2A)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)
Background of TOP2A antibody
DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIalpha and Topo IIbeta. The 180 kDa isoform, Topo IIbeta, and the 170 kDa isoform, Topo IIalpha, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.
General readings
Yoshida,K., et al., (2006) Mol. Cell. Biol. 26 (9), 3414-3431
Immunoperoxidase of monoclonal antibody to TOP2A on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to TOP2A on HeLa cell. [antibody concentration 10 ug/ml]
TOP2A monoclonal antibody (M01), clone 1E2 Western Blot analysis of TOP2A expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged TOP2A is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-TOP2A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. TOP2A is supported by BioGPS gene expression data to be expressed in HepG2.; TOP2A antibody - C-terminal region (AVARP04007_T100) in Human HepG2 cells using Western Blot
Immunohistochemistry (IHC) analyzes of Topo IIalpha antibody (Cat.-No.: AP01227PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Topo IIalpha antibody (Cat.-No.: AP01227PU-N) in extracts from LOVO cells.
Immunohistochemistry (IHC) analyzes of Topo IIα antibody (Cat.-No.: AP01370PU-N) in paraffin-embedded human liver carcinoma tissue
Western blot (WB) analysis of Topo IIα antibody (Cat.-No.: AP01370PU-N) in extracts from HT-29.
Immunoprecipitation ( Native & denatured ) with TOP2A polyclonal antibody ( Cat # PAB13698 ). Use Protein A. Use 10 µg/mg Raji cell lysate.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to TOP2A on HeLa cell (antibody concentration 10 µg/ml).
TOP2A monoclonal antibody clone 1E2 Western Blot analysis of TOP2A expression in HeLa NE.
Detection limit for recombinant GST tagged TOP2A is approximately 0.3 ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (AP54331PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of TOP2A Antibody (C-term) (AP54331PU-N) in A375 cell line lysates (35 µg/lane). TOP2A (arrow) was detected using the purified Pab.
TOP2A/Topo? Antibody (C-term) (AP54332PU-N) western blot analysis in K562 cell line lysates (35 µg/lane). This demonstrates the TOP2A/Topo? antibody detected the TOP2A/Topo? protein (arrow).
Formalin-Fixed, Paraffin-Embedded Human lymph node stained with TOP2A antibody Cat.-No. AM10144SU-N using peroxidase conjugate and AEC chromogen. Note nuclear staining of germinal center B-cells.
Immunohistochemical detection of Topo IIα on paraffin embedded section of a human stomach cancer with AM26449SU-N.
Western blot analysis of Topo IIα expression in MRC5 at various times following serum stimulation of arrested cells using AM26449SU-N and anti-Cyclin D1.
Western blot analysis of Topo IIα expression in Jurkat (1), HL-60 (2) and IC2Tr (3) using AM26449SU-N.
Western blot analysis of phospho-DNA expression in Jurkat cells using AM26582AF-N
Immunohistochemical detection on DNA Topoisomerase II alpha on paraffin embedded section of human stomach with AM26595AF-N
Immunocytochemical detection of DNA Topoisomerase II alpha on acetone fixed HEp-II with AM26595AF-N.
Western blot analysis of DNA Topoisomerase II alpha expression in Jurkat (1), Raji (2) and WR10L (3) using AM26595AF-N.
Flow cytometric analysis of DNA Topoisomerase II alpha expression in Jurkat. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26595AF-N to the cells.
Western blot analysis of Topo IIα, β expression in Jurkat (1), (3) and Raji (2), (4) using AM26690AF-N ((1),(2); 5 μg/ml and (3),(4); 10 μg/ml).
Western blot analysis of extracts from HepG2 cells treated with Ca2+ using TOP2A (Phospho-Thr1343) Antibody AP55782PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802384)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802385)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802450)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802499) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802505) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (TA802514) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802515)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802516)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802517)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802536)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (TA802537) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802684)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
Western blot analysis of extracts from HepG2 cells, using TOP2A (Ab-1343) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from K562 cells (Lane 2), using TOP2A (Ab-1343) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from HT-29 cells, using TOP2A antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using TOP2A antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using TOP2A antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from LOVO cells, using TOP2A (Ab-1106) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TOP2A (Ab-1106) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from A549 cells, Jurkat cells, HeLa cells, HepG2 cells, COLO205 cells and HuvEc cells, using LIPB1 antibody.The lane on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human ovarian and colon cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using TOP2A antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human ovarian and colon cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using TOP2A antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis on Jurkat cell lysate using TOP2A Antibody
Western blot analysis of extracts from HepG2 cells, treated with Ca2+ (40nM, 30mins), using TOP2A (Phospho-Thr1343) antibody.The lane on the right is treated with the synthesized peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA802384 | TOP2A antibody |
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| TOP2A mouse monoclonal antibody, clone OTI2A9 (formerly 2A9) | |||||||
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Mouse | IgG2b | OTI2A9 | Purified | Hu | P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +13 additional images |
Top Selling product! Highly validated antibody!
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| UM800042 | TOP2A antibody |
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| TOP2A mouse monoclonal antibody,clone UMAB145 | |||||||
 |
Mouse | IgG1 | UMAB145 | Purified | Hu | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
|
||||||
| UM800043 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody,clone UMAB146 | |||||||
 |
Mouse | IgG1 | UMAB146 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| UM870042 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody,clone UMAB145 | |||||||
|
Mouse | IgG1 | UMAB145 | Purified | Hu | P |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
|
||||||
| UM870043 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody,clone UMAB146 | |||||||
|
Mouse | IgG1 | UMAB146 | Purified | Hu | P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA802385 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | |||||||
 |
Mouse | IgG1 | OTI2F8 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
||||||
| TA802450 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTI1D5 (formerly 1D5) | |||||||
 |
Mouse | IgG1 | OTI1D5 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
||||||
| TA802499 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTIE1 (formerly E1) | |||||||
 |
Mouse | IgG1 | OTIE1 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA802505 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTIB4 (formerly B4) | |||||||
 |
Mouse | IgG1 | OTIB4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA802515 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTI5E6 (formerly 5E6) | |||||||
 |
Mouse | IgG1 | OTI5E6 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
||||||
| TA802516 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTI2D12 (formerly 2D12) | |||||||
 |
Mouse | IgG1 | OTI2D12 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
||||||
| TA802536 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTI19D4 (formerly 19D4) | |||||||
 |
Mouse | IgG1 | OTI19D4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA802537 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTIE11 (formerly E11) | |||||||
 |
Mouse | IgG1 | OTIE11 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA802684 | TOP2A antibody |
||||||
| TOP2A mouse monoclonal antibody, clone OTI1D2 (formerly 1D2) | |||||||
 |
Mouse | IgG1 | OTI1D2 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
||||||
| AM20953PU-N | TOP2A antibody |
||||||
 |
Mouse | IgG1 | 1E2 | Hu | E, ICC/IF, P, WB |
50 µg /
€530.00
|
|
| Acris Antibodies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
||||||
| AM26595AF-N | TOP2A (C-term) antibody |
||||||
 |
Mouse | IgG2a | 1C5 | Azide Free | Hu | F, ICC/IF, IP, P, WB |
0.1 mg /
€390.00
|
| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA313198 | TOP2A antibody |
||||||
| Rabbit polyclonal TOP2A (Ab-1343) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA313200 | TOP2A antibody |
||||||
| Rabbit polyclonal anti-TOP2A antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA313202 | TOP2A antibody |
||||||
| Rabbit polyclonal TOP2A (Thr1343) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313203 | TOP2A antibody |
||||||
| Rabbit polyclonal TOP2A (Ab-1106) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
