TOP3B antibody

Principal name

TOP3B antibody

Alternative names for TOP3B antibody

TOP3B1, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1

SwissProt ID

O95985 (Human), Q9Z321 (Mouse)

Gene ID

8940 (TOP3B)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of TOP3B antibody

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I and DNA topoisomerase II relax both positive and negative supercoils, DNA topoisomerase III relaxes only negative supercoils. DNA topoisomerase III alpha exists as a long and a short isoform, which are produced by alternative splicing. DNA topoisomerase III alpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. DNA topoisomerase III beta exists as three isoforms, namely beta1, beta2 and beta3, also produced by alternative splicing. DNA topoisomerase III beta1 is expressed in testes, heart and skeletal muscle, whereas beta2 is expressed in thymus, kidney and pancreas.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TOP3B antibody

Western blot analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2b 4F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOP3B antibody

Western blot analysis of extracts from HepG2 cells, COLO205 cells and HUVEC cells, using TOP3B antibodyThe lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TOP3B antibody

Western blot (WB) analysis of Topo IIIβ-1 antibody (Cat.-No.: AP06654PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

TOP3B (C-term) antibody

TOP3B Antibody (C-term) (AP54334PU-N) western blot analysis in mouse bladder tissue lysates (35 µg/lane). This demonstrates the TOP3B antibody detected the TOP3B protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

TOP3B antibody

Western Blot analysis of TOP3B expression in transfected 293T cell line (H00008940-T01) by TOP3B MaxPab polyclonal antibody.

Lane 1: TOP3B transfected lysate(94.82 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

TOP3B antibody

Western Blot analysis of TOP3B expression in transfected 293T cell line (H00008940-T02) by TOP3B MaxPab polyclonal antibody.

Lane 1: TOP3B transfected lysate(96.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

TOP3B antibody

Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M03), clone 1H8.

Lane 1: TOP3B transfected lysate(96.662 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOP3B antibody

Immunofluorescence of monoclonal antibody to TOP3B on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG2a 1H4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOP3B antibody

Immunofluorescence of monoclonal antibody to TOP3B on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG2a 4H7 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOP3B antibody

Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M06), clone 2G2.

Lane 1: TOP3B transfected lysate(96.662 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

TOP3B antibody

Western blot analysis of extracts from HepG2/COLO205/HuvEc cells, using TOP3B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

TOP3B antibody

Western blot analysis of extracts from HepG2/COLO205/HuvEc cells, using TOP3B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

TOP3B antibody

Immunohistochemical analysis of Topoisomerase 3 beta1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

TOP3B antibody

Immunohistochemical analysis of Topoisomerase 3 beta1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

TOP3B antibody

Immunohistochemical analysis of Topoisomerase 3 beta1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

TOP3B antibody

Western Blot: Topo IIIbeta-1 Antibody [NB100-92303] - Western blot (WB) analysis of Topo IIIâ-1 (Q84)  antibody in extracts from HepG2 cells. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

TOP3B antibody

TOP3B Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

TOP3B antibody

TOP3B Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TOP3B

TOP3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TOP3B

TOP3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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