TP53 / p53 antibody

Principal name

TP53 / p53 antibody

Alternative names for TP53 / p53 antibody

Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

SwissProt ID

P02340 (Mouse), P04637 (Human), P07193 (Xenla), P10360 (Chick), P10361 (Rat), P41685 (Felca), P51664 (Sheep), P67939 (Bovin), P79892 (Horse), Q29537 (Canfa), Q95330 (Rabit), Q9TUB2 (Pig), Q9WUR6 (Cavpo)

Gene ID

7157 (TP53), 24842 (Tp53), 22059 (Trp53), 397926 (tp53)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Mky (Monkey), Bov (Bovine), Can (Canine), Hst (Hamster), Por (Porcine), Rb (Rabbit), Prim (Primate), Av (Birds), Eq (Equine), Fe (Feline), Frog, Sh (Sheep), Ye (Yeast), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Sheep, Rat, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), ChIP assay (ChIP), Radioimmunoassay (R), PLA/Proximity ligation assay (PLA), Dot blot (Dot)

Background of TP53 / p53 antibody

The 393 amino acid tumor suppressor phosphoprotein p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. The level of p53 protein is low in normal cells but increases following DNA damage or cellular stress. Mutations in the p53 gene are found in more than 50% of human cancers. p53 acts to arrest the cell cycle or induce apoptosis by regulating the transcription of specific genes. p53 can also initiate apoptosis through a direct signaling pathway. The p53 tumor suppressor gene encodes a transcription factor that contributes to several cellular activities that include apoptosis, transient growth arrest, and sustained growth arrest or senescence. Mutations within the p53 gene are found in about half of all human cancers. In cells that are functioning normally the MDM2 protein binds to p53 and maintains p53 at low levels by increasing its susceptibility to proteolysis by the 26S proteosome. A cell that undergoes stress loses the ability of MDM2 to bind to p53 and as a result p53 levels increase which then leads to cell cycle arrest or apoptosis. p53 induced cell cycle arrest or apoptosis can be achieved through transcriptional regulation of several genes including the cell cycle inhibitor p21, DNA repair gene GADD45, and the apoptotic inducer Bax. Besides MDM2 inactivation, p53 can also be functionally inactivated by mutation or binding to DNA tumor virus encoded proteins, such as SV40 large T antigen, Adenovirus E1B and papilloma virus E6 proteins.

General readings

Kohn KW Molecular interaction map of the mammalian cell cycle control and DNA repair systems. Mol Biol Cell 10:2703-34 (1999). PubMed: 10436023
Lu H et al. The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro. Mol Cell Biol 17:5923-34 (1997). PubMed: 9315650
Hao M et al. Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem 271:29380-5 (1996). PubMed: 8910602
Lohrum M & Scheidtmann KH Differential effects of phosphorylation of rat p53 on transactivation of promoters derived from different p53 responsive genes. Oncogene 13:2527-39 (1996). PubMed: 9000127

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502870) Mouse IgG1 OTI5E2 Purified Hu, Mky, Ms F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TP53 / p53 pSer392 antibody

Figure 1. Immunohistochemistry staining of Wild-type p53 expressed in Human Trophoblast (Paraffin-Embedded Sections): 1A: anti-p53 antibody (Total), 1B: anti-p53 antibody (Phospho Ser392). 
Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. Mouse IgG1 FP3.2 Aff - Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

TP53 / p53 pSer6 antibody

Western blot analysis of extracts from HT29 and Hela cells untreated or treated with UV using p53(Phospho-Ser6) Antibody Cat.-No AP08001PU Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

TP53 / p53 (acetyl K382) antibody

MCF-7 cells were treated with 50 µM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with Rabbit polyclonal Antibody against Acetylated p53 (Lys382). Lane 1: Untreated, control MCF-7 cells; Lane 2: Treated MCF-7 cells. Proteins were visualized using a Donkey anti-Rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu WB
50 µl / €260.00
  Acris Antibodies GmbH

TP53 / p53 (16-25) antibody

Confocal Microscopy of Human HeLa cells using BM4079F p53 antibody (BP53-12, FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC antibody. Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic. Mouse IgG2a BP53-12 FITC Hu, Prim ICC/IF
0.1 mg / €290.00
  Acris Antibodies GmbH

TP53 / p53 (16-25) antibody

Confocal Microscopy of Human HeLa cells using BM4079F p53 antibody (BP53-12, FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC antibody. Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic. Mouse IgG2a BP53-12 Purified Hu, Prim C, E, ICC/IF, IP, P, WB
0.1 mg / €220.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) antibody

Formalin-Paraffin Human colon stained with p53 Antibody Cat.-No SM2139P (Clone DO-7). Mouse IgG2b DO-7 Purified Bov, Hu, Mky C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) antibody

Formalin-Paraffin Human colon stained with p53 Antibody Cat.-No SM2139P (Clone DO-7). Mouse IgG2b DO-7 Purified Bov, Hu, Mky C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) antibody

Formalin-Paraffin Human colon stained with p53 Antibody Cat.-No SM2139P (Clone DO-7). Mouse IgG2b DO-7 Purified Bov, Hu, Mky C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) antibody

Formalin-Fixed, Paraffin-Embedded colon Ca stained with p53 Antibody Cat.-No SM2139S (Clone DO-7). Mouse IgG2b DO-7 Supernatant Bov, Hu, Mky C, F, ICC/IF, IP, P, WB
1.0 ml / €360.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) antibody

Formalin-Fixed, Paraffin-Embedded colon Ca stained with p53 Antibody Cat.-No SM2139S (Clone DO-7). Mouse IgG2b DO-7 Supernatant Bov, Hu, Mky C, F, ICC/IF, IP, P, WB
0.1 ml / €150.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) antibody

Formalin-Fixed, Paraffin-Embedded colon Ca stained with p53 Antibody Cat.-No SM2139S (Clone DO-7). Mouse IgG2b DO-7 Supernatant Bov, Hu, Mky C, F, ICC/IF, IP, P, WB
0.5 ml / €280.00
  Acris Antibodies GmbH

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Human normal lung tissue and lung adenocarcinoma tissue using anti-TP53 mouse monoclonal antibody. (UM500049; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB55 Purified Hu ICC/IF, P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Human normal colon tissue and colorectal cancer tissue using anti-TP53 mouse monoclonal antibody. (UM500053; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB62 Purified Hu ICC/IF, P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 Mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800098)(1:200) Mouse IgG1 UMAB206 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 Mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800098)(1:200) Mouse IgG1 UMAB206 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502780) Mouse IgG1 OTI3D5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502781) Mouse IgG1 OTI2C4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TP53 / p53 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502782) Mouse IgG1 OTI1C6 Purified Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

TP53 / p53 antibody

Anti-TP53 mouse monoclonal antibody (TA502783) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53(RC200003). Mouse IgG1 OTI2E4 Purified Hu, Mky, Ms F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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