TPCN1 antibody

Principal name

TPCN1 antibody

Alternative names for TPCN1 antibody

Two pore calcium channel protein 1, TPC1, KIAA1169, Voltage-dependent calcium channel protein TPC1

SwissProt ID

Q9ULQ1 (Human)

Gene ID

53373 (TPCN1), 246215 (Tpcn1)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TPCN1 antibody

Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated + (v) and Ca2+ (Cav) channels. These twelve transmembrane structures are further thought to form functiol dimers. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptiolly high in spleen, lung, liver, and kidney. Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (ADP) are responsible for the intracellular changes in Ca2+ ion concentration. In contrast to the other Ca2+-mobilizing agents, ADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes). Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the ADP-induced release of Ca2+ . Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon ADP stimulation. Filly, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of ADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ . As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TPCN1 antibody

Western blot analysis of rat heart lysate (lanes 1 and 3) and rat liver membrane (lanes 2 and 4): 1, 2. Anti-Two Pore Calcium Channel Protein 1 (extracellular) antibody, (1:200). 3, 4. Anti-Two Pore Calcium Channel Protein 1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

TPCN1 antibody

WB Suggested Anti-TPCN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

TPCN1 antibody

Human HT1080; Host: Rabbit. Target Name: TPCN1. Sample Tissue: HT1080. Antibody Dilution: 1.0ug/ml.; TPCN1 antibody - N-terminal region (ARP35276_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

TPCN1 antibody

TPCN1 Rabbit Serum Hu P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

TPCN1 antibody

TPCN1 Goat Purified Hu, Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

TPCN1 antibody

Immunohistochemistry-Paraffin: TPCN1 Antibody [NBP1-83642] - Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in renal tubules. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

6 Item(s)

per page
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