TPCN2 antibody

Principal name

TPCN2 antibody

Alternative names for TPCN2 antibody

Two pore calcium channel protein 2, TPC2, Voltage-dependent calcium channel protein TPC2

SwissProt ID

Q8NHX9 (Human)

Gene ID

219931 (TPCN2), 233979 (Tpcn2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Sheep, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E)

Background of TPCN2 antibody

Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated + (v) and Ca2+ (Cav) channels. These twelve transmembrane structures are further thought to form functiol dimers. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptiolly high in spleen, lung, liver, and kidney. Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (ADP) are responsible for the intracellular changes in Ca2+ ion concentration. In contrast to the other Ca2+-mobilizing agents, ADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes). Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the ADP-induced release of Ca2+ . Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon ADP stimulation. Filly, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of ADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ . As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TPCN2 antibody

Western blot analysis of MCF-7 cell lysates with anti-Tpcn2 antibody at 1:500 dilution. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

TPCN2 antibody

Western blot analysis of mouse kidney lysate (lanes 1 and 4), rat lung membrane (lanes 2 and 5) and human embryonic Kidney 293 cell lysate (lanes 3 and 6): 1-3. Anti-Two Pore Calcium Channel Protein 2 (extracellular) antibody, (1:200). 4-6. Anti-Two Pore Calcium Channel Protein 2 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

TPCN2 (735-747) antibody

Pancreas, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hu, Mky, Prim P
50 µg / €470.00
  Acris Antibodies GmbH

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